Comparaison des premières étapes de l’embryogenèse somatique chez Baillonella toxisperma et Vitellaria paradoxa (Sapotacées)
Comparison of first stages of somatic embryogenesis in Baillonella toxisperma and Vitellaria paradoxa. In order to initiate the in vitro propagation of Baillonella toxisperma and Vitellaria paradoxa, a comparative study of somatic embryogenesis was studied on these two Sapotaceae using leaf fragment...
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| Format: | Article |
| Language: | English |
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Presses Agronomiques de Gembloux
2008-01-01
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| Series: | Biotechnologie, Agronomie, Société et Environnement |
| Subjects: | |
| Online Access: | http://www.pressesagro.be/base/text/v12n2/131.pdf |
| _version_ | 1819068463514124288 |
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| author | Ndoumou DO. Tchinda ND. Fotso Sanonne |
| author_facet | Ndoumou DO. Tchinda ND. Fotso Sanonne |
| author_sort | Ndoumou DO. |
| collection | DOAJ |
| description | Comparison of first stages of somatic embryogenesis in Baillonella toxisperma and Vitellaria paradoxa. In order to initiate the in vitro propagation of Baillonella toxisperma and Vitellaria paradoxa, a comparative study of somatic embryogenesis was studied on these two Sapotaceae using leaf fragment from plants under production. A culture medium of Murashige and Skoog half strength (MS/2) supplemented with 0.6% agar and 4.5% sucrose was used as the basic medium. 0.5 mg.l-1 of 6-benzylaminopurine (BAP) and 0.5 to 5 mg.l-1 of 2.4-dichlorophenoxyacetic acid (2.4-D) were added to the basic medium to study the process of callogenesis and the formation of the somatic embryo on the callus. The BAP/2.4-D combination significantly affected callogenesis in both species. During 28 days of culture, the most reactive combinations are 0.5/0.5 in B. toxisperma which allowed 75.2% of the explants to form callus, and 0.5/3 in V. paradoxa which permitted to obtain up to 87.3% callogenesis. At 3 mg.l-1, 2.4-D permitted the differentiation of somatic embryos in 92.7% of callus in B. toxisperma with an average of 38 embryos per callus. On the contrary in V. paradoxa, the most reaction is observed with the combination of 0.5/2 BAP/2.4-D which permitted to obtain 62.1% of embryonic callus in 97 days of culture with an average of 27 embryos per callus. The histological study of embryonic callus showed that the development of the differentiated somatic embryos occurs through the same stages in both species; notably the nodular stage, the division of nodular cells stage and the bipolar embryos stage, thus the respective percentages obtained are 77.2% and 66.8% in B. toxisperma and V. paradoxa. Though this work opens probably new way of propagation of these two species, the transformation of the bipolar embryos into viable plants remains important phase to study. |
| first_indexed | 2024-12-21T16:34:33Z |
| format | Article |
| id | doaj.art-931f20d309744617aaf810f12fcd1519 |
| institution | Directory Open Access Journal |
| issn | 1370-6233 1780-4507 |
| language | English |
| last_indexed | 2024-12-21T16:34:33Z |
| publishDate | 2008-01-01 |
| publisher | Presses Agronomiques de Gembloux |
| record_format | Article |
| series | Biotechnologie, Agronomie, Société et Environnement |
| spelling | doaj.art-931f20d309744617aaf810f12fcd15192022-12-21T18:57:15ZengPresses Agronomiques de GemblouxBiotechnologie, Agronomie, Société et Environnement1370-62331780-45072008-01-01122131138Comparaison des premières étapes de l’embryogenèse somatique chez Baillonella toxisperma et Vitellaria paradoxa (Sapotacées)Ndoumou DO.Tchinda ND.FotsoSanonneComparison of first stages of somatic embryogenesis in Baillonella toxisperma and Vitellaria paradoxa. In order to initiate the in vitro propagation of Baillonella toxisperma and Vitellaria paradoxa, a comparative study of somatic embryogenesis was studied on these two Sapotaceae using leaf fragment from plants under production. A culture medium of Murashige and Skoog half strength (MS/2) supplemented with 0.6% agar and 4.5% sucrose was used as the basic medium. 0.5 mg.l-1 of 6-benzylaminopurine (BAP) and 0.5 to 5 mg.l-1 of 2.4-dichlorophenoxyacetic acid (2.4-D) were added to the basic medium to study the process of callogenesis and the formation of the somatic embryo on the callus. The BAP/2.4-D combination significantly affected callogenesis in both species. During 28 days of culture, the most reactive combinations are 0.5/0.5 in B. toxisperma which allowed 75.2% of the explants to form callus, and 0.5/3 in V. paradoxa which permitted to obtain up to 87.3% callogenesis. At 3 mg.l-1, 2.4-D permitted the differentiation of somatic embryos in 92.7% of callus in B. toxisperma with an average of 38 embryos per callus. On the contrary in V. paradoxa, the most reaction is observed with the combination of 0.5/2 BAP/2.4-D which permitted to obtain 62.1% of embryonic callus in 97 days of culture with an average of 27 embryos per callus. The histological study of embryonic callus showed that the development of the differentiated somatic embryos occurs through the same stages in both species; notably the nodular stage, the division of nodular cells stage and the bipolar embryos stage, thus the respective percentages obtained are 77.2% and 66.8% in B. toxisperma and V. paradoxa. Though this work opens probably new way of propagation of these two species, the transformation of the bipolar embryos into viable plants remains important phase to study.http://www.pressesagro.be/base/text/v12n2/131.pdf2.4-dichlorophenoxyacetic acid6-benzylaminopurineBaillonella toxispermabipolar somatic embryoscallogenesisVitellaria paradoxa |
| spellingShingle | Ndoumou DO. Tchinda ND. Fotso Sanonne Comparaison des premières étapes de l’embryogenèse somatique chez Baillonella toxisperma et Vitellaria paradoxa (Sapotacées) Biotechnologie, Agronomie, Société et Environnement 2.4-dichlorophenoxyacetic acid 6-benzylaminopurine Baillonella toxisperma bipolar somatic embryos callogenesis Vitellaria paradoxa |
| title | Comparaison des premières étapes de l’embryogenèse somatique chez Baillonella toxisperma et Vitellaria paradoxa (Sapotacées) |
| title_full | Comparaison des premières étapes de l’embryogenèse somatique chez Baillonella toxisperma et Vitellaria paradoxa (Sapotacées) |
| title_fullStr | Comparaison des premières étapes de l’embryogenèse somatique chez Baillonella toxisperma et Vitellaria paradoxa (Sapotacées) |
| title_full_unstemmed | Comparaison des premières étapes de l’embryogenèse somatique chez Baillonella toxisperma et Vitellaria paradoxa (Sapotacées) |
| title_short | Comparaison des premières étapes de l’embryogenèse somatique chez Baillonella toxisperma et Vitellaria paradoxa (Sapotacées) |
| title_sort | comparaison des premieres etapes de l embryogenese somatique chez baillonella toxisperma et vitellaria paradoxa sapotacees |
| topic | 2.4-dichlorophenoxyacetic acid 6-benzylaminopurine Baillonella toxisperma bipolar somatic embryos callogenesis Vitellaria paradoxa |
| url | http://www.pressesagro.be/base/text/v12n2/131.pdf |
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