Orchid seeds viability identification by tetrazolium test
The knowledge of germinal potential optimizes the practice of in vitro germination of Orchidaceae. This work evaluated of the type and concentrations of tetrazolium solutions on reaction time and on the identification of viable orchid seeds. Were used seeds of the species Brassavola tuberculata Rchb...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2014-10-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | http://www.uel.br/revistas/uel/index.php/semagrarias/editor/submission/11627 |
| _version_ | 1811312785266573312 |
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| author | Jackeline Schultz Soares Yara Brito Chaim Jardim Rosa Mariana Bento Tatara José Carlos Sorgato Camila Soares Rosa Lemes |
| author_facet | Jackeline Schultz Soares Yara Brito Chaim Jardim Rosa Mariana Bento Tatara José Carlos Sorgato Camila Soares Rosa Lemes |
| author_sort | Jackeline Schultz Soares |
| collection | DOAJ |
| description | The knowledge of germinal potential optimizes the practice of in vitro germination of Orchidaceae. This work evaluated of the type and concentrations of tetrazolium solutions on reaction time and on the identification of viable orchid seeds. Were used seeds of the species Brassavola tuberculata Rchb f., Cattleya labiata Lindley var Amoema, Cattleya intermedia and Dendrobium antenatum and of two primary hybrids of Cattleya labiata autumnalis x Cattleya labiata Lindley var. Alba. The seeds (0.01g) of each species or hybrid were soaked in 3 mL of aqueous and neutral solutions of 2, 3, 5 triphenyltetrazolium chloride at 0.0625%, 0.125%, 0.25%, 0.5% ou 1,0% concentrations and stored in a light-free environment at 25±2 °C, being evaluated every 30 minutes for identification of the time needed for embryos coloring and later counting of the number of viable seeds by milligram of seed. A completely randomized design was used, in a factorial scheme 6 x 2 x 5 (six genotypes of orchids, two types of tetrazolium solutions and five concentrations) with 3 replications. The genotypes responded differently response both for viable seeds by milligram of seed and for the time required for embryos coloring and C. intermedia not respond to the test. Irrespective of solution type, concentrations only interfered in reaction time for the hybrids. It is recommended, therefore, the use of neutral tetrazolium solution at 0.5% for indentification of viable seeds of hybrids of Cattleya labiata autumnalis x Cattleya labiata var. Alba and use of aqueous solution at 0.125% for Brassavola tuberculata, Cattleya labiata var Amoema e Dendrobium antenatum. |
| first_indexed | 2024-04-13T10:41:31Z |
| format | Article |
| id | doaj.art-931f298c3c2440cdbee707eceaabb8dd |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-04-13T10:41:31Z |
| publishDate | 2014-10-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-931f298c3c2440cdbee707eceaabb8dd2022-12-22T02:49:54ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592014-10-013552275228410.5433/1679-0359.2014v35n5p2275Orchid seeds viability identification by tetrazolium testJackeline Schultz Soares0Yara Brito Chaim Jardim Rosa1Mariana Bento Tatara2José Carlos Sorgato3Camila Soares Rosa Lemes 4Universidade Federal da Grande DouradosUniversidade Federal da Grande DouradosFaculdade de Ciências da SaúdeUniversidade Federal da Grande DouradosUniversidade Federal da Grande DouradosThe knowledge of germinal potential optimizes the practice of in vitro germination of Orchidaceae. This work evaluated of the type and concentrations of tetrazolium solutions on reaction time and on the identification of viable orchid seeds. Were used seeds of the species Brassavola tuberculata Rchb f., Cattleya labiata Lindley var Amoema, Cattleya intermedia and Dendrobium antenatum and of two primary hybrids of Cattleya labiata autumnalis x Cattleya labiata Lindley var. Alba. The seeds (0.01g) of each species or hybrid were soaked in 3 mL of aqueous and neutral solutions of 2, 3, 5 triphenyltetrazolium chloride at 0.0625%, 0.125%, 0.25%, 0.5% ou 1,0% concentrations and stored in a light-free environment at 25±2 °C, being evaluated every 30 minutes for identification of the time needed for embryos coloring and later counting of the number of viable seeds by milligram of seed. A completely randomized design was used, in a factorial scheme 6 x 2 x 5 (six genotypes of orchids, two types of tetrazolium solutions and five concentrations) with 3 replications. The genotypes responded differently response both for viable seeds by milligram of seed and for the time required for embryos coloring and C. intermedia not respond to the test. Irrespective of solution type, concentrations only interfered in reaction time for the hybrids. It is recommended, therefore, the use of neutral tetrazolium solution at 0.5% for indentification of viable seeds of hybrids of Cattleya labiata autumnalis x Cattleya labiata var. Alba and use of aqueous solution at 0.125% for Brassavola tuberculata, Cattleya labiata var Amoema e Dendrobium antenatum.http://www.uel.br/revistas/uel/index.php/semagrarias/editor/submission/11627Flowering; Orchidaceae; Seed viability test |
| spellingShingle | Jackeline Schultz Soares Yara Brito Chaim Jardim Rosa Mariana Bento Tatara José Carlos Sorgato Camila Soares Rosa Lemes Orchid seeds viability identification by tetrazolium test Semina: Ciências Agrárias Flowering; Orchidaceae; Seed viability test |
| title | Orchid seeds viability identification by tetrazolium test |
| title_full | Orchid seeds viability identification by tetrazolium test |
| title_fullStr | Orchid seeds viability identification by tetrazolium test |
| title_full_unstemmed | Orchid seeds viability identification by tetrazolium test |
| title_short | Orchid seeds viability identification by tetrazolium test |
| title_sort | orchid seeds viability identification by tetrazolium test |
| topic | Flowering; Orchidaceae; Seed viability test |
| url | http://www.uel.br/revistas/uel/index.php/semagrarias/editor/submission/11627 |
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