Untargeted Microbial Exometabolomics and Metabolomics Analysis of <i>Helicobacter pylori</i> J99 and <i>jhp0106</i> Mutant
Untargeted metabolomic profiling provides the opportunity to comprehensively explore metabolites of interest. Herein, we investigated the metabolic pathways associated with Jhp0106, a glycosyltransferase enzyme in <i>Helicobacter pylori</i>. Through untargeted exometabolomic and metabolo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/12/808 |
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| author | Cheng-Yen Kao Pei-Yun Kuo Hsiao-Wei Liao |
| author_facet | Cheng-Yen Kao Pei-Yun Kuo Hsiao-Wei Liao |
| author_sort | Cheng-Yen Kao |
| collection | DOAJ |
| description | Untargeted metabolomic profiling provides the opportunity to comprehensively explore metabolites of interest. Herein, we investigated the metabolic pathways associated with Jhp0106, a glycosyltransferase enzyme in <i>Helicobacter pylori</i>. Through untargeted exometabolomic and metabolomic profiling, we identified 9 and 10 features with significant differences in the culture media and pellets of the wild-type (WT) J99 and <i>jhp0106</i> mutant (Δ<i>jhp0106</i>). After tentative identification, several phosphatidylethanolamines (PEs) were identified in the culture medium, the levels of which were significantly higher in WT J99 than in Δ<i>jhp0106</i>. Moreover, the reduced lysophosphatidic acid absorption from the culture medium and the reduced intrinsic diacylglycerol levels observed in Δ<i>jhp0106</i> indicate the possibility of reduced PE synthesis in Δ<i>jhp0106</i>. The results suggest an association of the PE synthesis pathway with flagellar formation in <i>H. pylori</i>. Further investigations should be conducted to confirm this finding and the roles of the PE synthesis pathway in flagellar formation. This study successfully demonstrates the feasibility of the proposed extraction procedure and untargeted exometabolomic and metabolomic profiling strategies for microbial metabolomics. They may also extend our understanding of metabolic pathways associated with flagellar formation in <i>H. pylori</i>. |
| first_indexed | 2024-03-10T03:35:24Z |
| format | Article |
| id | doaj.art-93225f0ebf2946b08c219cc7689bee4f |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T03:35:24Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-93225f0ebf2946b08c219cc7689bee4f2023-11-23T09:31:10ZengMDPI AGMetabolites2218-19892021-11-01111280810.3390/metabo11120808Untargeted Microbial Exometabolomics and Metabolomics Analysis of <i>Helicobacter pylori</i> J99 and <i>jhp0106</i> MutantCheng-Yen Kao0Pei-Yun Kuo1Hsiao-Wei Liao2Institute of Microbiology and Immunology, School of Life Science, National Yang Ming Chiao Tung University, Taipei 122, TaiwanInstitute of Microbiology and Immunology, School of Life Science, National Yang Ming Chiao Tung University, Taipei 122, TaiwanDepartment of Pharmacy, National Yang Ming Chiao Tung University, Taipei 122, TaiwanUntargeted metabolomic profiling provides the opportunity to comprehensively explore metabolites of interest. Herein, we investigated the metabolic pathways associated with Jhp0106, a glycosyltransferase enzyme in <i>Helicobacter pylori</i>. Through untargeted exometabolomic and metabolomic profiling, we identified 9 and 10 features with significant differences in the culture media and pellets of the wild-type (WT) J99 and <i>jhp0106</i> mutant (Δ<i>jhp0106</i>). After tentative identification, several phosphatidylethanolamines (PEs) were identified in the culture medium, the levels of which were significantly higher in WT J99 than in Δ<i>jhp0106</i>. Moreover, the reduced lysophosphatidic acid absorption from the culture medium and the reduced intrinsic diacylglycerol levels observed in Δ<i>jhp0106</i> indicate the possibility of reduced PE synthesis in Δ<i>jhp0106</i>. The results suggest an association of the PE synthesis pathway with flagellar formation in <i>H. pylori</i>. Further investigations should be conducted to confirm this finding and the roles of the PE synthesis pathway in flagellar formation. This study successfully demonstrates the feasibility of the proposed extraction procedure and untargeted exometabolomic and metabolomic profiling strategies for microbial metabolomics. They may also extend our understanding of metabolic pathways associated with flagellar formation in <i>H. pylori</i>.https://www.mdpi.com/2218-1989/11/12/808microbial metabolomicsexometabolomicsliquid chromatography-mass spectrometry<i>Helicobacter pylori</i>flagellum<i>jhp0106</i> mutant |
| spellingShingle | Cheng-Yen Kao Pei-Yun Kuo Hsiao-Wei Liao Untargeted Microbial Exometabolomics and Metabolomics Analysis of <i>Helicobacter pylori</i> J99 and <i>jhp0106</i> Mutant Metabolites microbial metabolomics exometabolomics liquid chromatography-mass spectrometry <i>Helicobacter pylori</i> flagellum <i>jhp0106</i> mutant |
| title | Untargeted Microbial Exometabolomics and Metabolomics Analysis of <i>Helicobacter pylori</i> J99 and <i>jhp0106</i> Mutant |
| title_full | Untargeted Microbial Exometabolomics and Metabolomics Analysis of <i>Helicobacter pylori</i> J99 and <i>jhp0106</i> Mutant |
| title_fullStr | Untargeted Microbial Exometabolomics and Metabolomics Analysis of <i>Helicobacter pylori</i> J99 and <i>jhp0106</i> Mutant |
| title_full_unstemmed | Untargeted Microbial Exometabolomics and Metabolomics Analysis of <i>Helicobacter pylori</i> J99 and <i>jhp0106</i> Mutant |
| title_short | Untargeted Microbial Exometabolomics and Metabolomics Analysis of <i>Helicobacter pylori</i> J99 and <i>jhp0106</i> Mutant |
| title_sort | untargeted microbial exometabolomics and metabolomics analysis of i helicobacter pylori i j99 and i jhp0106 i mutant |
| topic | microbial metabolomics exometabolomics liquid chromatography-mass spectrometry <i>Helicobacter pylori</i> flagellum <i>jhp0106</i> mutant |
| url | https://www.mdpi.com/2218-1989/11/12/808 |
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