Diversity and Effectivity of Indigenous <i>Mesorhizobium</i> Strains for Chickpea (<i>Cicer arietinum</i> L.) in Myanmar
Chickpea (<i>Cicer arietinum</i> L.) is one of the world’s main leguminous crops that provide chief source of food for humans. In the present study, we characterized thirty isolates of indigenous chickpea rhizobia from Myanmar based on the sequence analysis of the bacterial 16S...
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MDPI AG
2020-02-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/10/2/287 |
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| author | Khin Myat Soe Aung Zaw Htwe Kyi Moe Abiko Tomomi Takeo Yamakawa |
| author_facet | Khin Myat Soe Aung Zaw Htwe Kyi Moe Abiko Tomomi Takeo Yamakawa |
| author_sort | Khin Myat Soe |
| collection | DOAJ |
| description | Chickpea (<i>Cicer arietinum</i> L.) is one of the world’s main leguminous crops that provide chief source of food for humans. In the present study, we characterized thirty isolates of indigenous chickpea rhizobia from Myanmar based on the sequence analysis of the bacterial 16S rRNA gene. The sequence analysis confirmed that all isolates were categorized and identified as the genus <i>Mesorhizobium</i> and they were conspecific with <i>M. plurifarium</i>, <i>M. muliense</i>, <i>M. tianshanense</i>, and <i>M</i>. sp. This is the first report describing <i>M. muliense, M. tianshanense,</i> and <i>M. plurifurium</i> from different geographical distribution of indigenous mesorhizobia of chickpea in Myanmar. In order to substitute the use of chemical fertilizers in legume production, there is a need for the production of Biofertilizers with rhizobial inoculants. The effectiveness of Myanmar <i>Mesorhizobim</i> strains isolated from soil samples of major chickpea growing areas of Myanmar for plant growth and nitrogen fixation were studied in pot experiments. The nodule dry weight and acetylene reduction activity of the plant inoculated with <i>Mesorhizobium tianshanense</i> SalCP19 was significantly higher than the other tested isolates in Yezin-4 chickpea variety. But, <i>Mesorhizobium</i> sp. SalCP17 was showed high level of acetylene reduction activity per plant in Yezin-6 chickpea variety. |
| first_indexed | 2024-12-21T16:46:07Z |
| format | Article |
| id | doaj.art-9322cbb28aed41909d619b1c994e7c5e |
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| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-12-21T16:46:07Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-9322cbb28aed41909d619b1c994e7c5e2022-12-21T18:56:59ZengMDPI AGAgronomy2073-43952020-02-0110228710.3390/agronomy10020287agronomy10020287Diversity and Effectivity of Indigenous <i>Mesorhizobium</i> Strains for Chickpea (<i>Cicer arietinum</i> L.) in MyanmarKhin Myat Soe0Aung Zaw Htwe1Kyi Moe2Abiko Tomomi3Takeo Yamakawa4Laboratory of Plant Nutrition, Department of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, JapanDepartment of Agronomy, Yezin Agricultural University, Yezin 15013, MyanmarDepartment of Agronomy, Yezin Agricultural University, Yezin 15013, MyanmarLaboratory of Agroecology, Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University, 111 Harumachi, Kasuya-machi, Kasuya-gun, Fukuoka 811-2307, JapanLaboratory of Plant Nutrition, Department of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, JapanChickpea (<i>Cicer arietinum</i> L.) is one of the world’s main leguminous crops that provide chief source of food for humans. In the present study, we characterized thirty isolates of indigenous chickpea rhizobia from Myanmar based on the sequence analysis of the bacterial 16S rRNA gene. The sequence analysis confirmed that all isolates were categorized and identified as the genus <i>Mesorhizobium</i> and they were conspecific with <i>M. plurifarium</i>, <i>M. muliense</i>, <i>M. tianshanense</i>, and <i>M</i>. sp. This is the first report describing <i>M. muliense, M. tianshanense,</i> and <i>M. plurifurium</i> from different geographical distribution of indigenous mesorhizobia of chickpea in Myanmar. In order to substitute the use of chemical fertilizers in legume production, there is a need for the production of Biofertilizers with rhizobial inoculants. The effectiveness of Myanmar <i>Mesorhizobim</i> strains isolated from soil samples of major chickpea growing areas of Myanmar for plant growth and nitrogen fixation were studied in pot experiments. The nodule dry weight and acetylene reduction activity of the plant inoculated with <i>Mesorhizobium tianshanense</i> SalCP19 was significantly higher than the other tested isolates in Yezin-4 chickpea variety. But, <i>Mesorhizobium</i> sp. SalCP17 was showed high level of acetylene reduction activity per plant in Yezin-6 chickpea variety.https://www.mdpi.com/2073-4395/10/2/287chickpeamyanmar16s rrna gene<i>mesorhizobium</i>nitrogen fixation |
| spellingShingle | Khin Myat Soe Aung Zaw Htwe Kyi Moe Abiko Tomomi Takeo Yamakawa Diversity and Effectivity of Indigenous <i>Mesorhizobium</i> Strains for Chickpea (<i>Cicer arietinum</i> L.) in Myanmar Agronomy chickpea myanmar 16s rrna gene <i>mesorhizobium</i> nitrogen fixation |
| title | Diversity and Effectivity of Indigenous <i>Mesorhizobium</i> Strains for Chickpea (<i>Cicer arietinum</i> L.) in Myanmar |
| title_full | Diversity and Effectivity of Indigenous <i>Mesorhizobium</i> Strains for Chickpea (<i>Cicer arietinum</i> L.) in Myanmar |
| title_fullStr | Diversity and Effectivity of Indigenous <i>Mesorhizobium</i> Strains for Chickpea (<i>Cicer arietinum</i> L.) in Myanmar |
| title_full_unstemmed | Diversity and Effectivity of Indigenous <i>Mesorhizobium</i> Strains for Chickpea (<i>Cicer arietinum</i> L.) in Myanmar |
| title_short | Diversity and Effectivity of Indigenous <i>Mesorhizobium</i> Strains for Chickpea (<i>Cicer arietinum</i> L.) in Myanmar |
| title_sort | diversity and effectivity of indigenous i mesorhizobium i strains for chickpea i cicer arietinum i l in myanmar |
| topic | chickpea myanmar 16s rrna gene <i>mesorhizobium</i> nitrogen fixation |
| url | https://www.mdpi.com/2073-4395/10/2/287 |
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