Development and Molecular Characterization of 55 Novel Polymorphic cDNA-SSR Markers in Faba Bean (Vicia faba L.) Using 454 Pyrosequencing
Faba bean (Vicia faba L.) is a major food source and fodder legume, popularly known for its high content of seed-protein. Its role is critical in crop rotation, and for fixing nitrogen effectively. Polymorphic simple sequence repeat markers from transcript sequences (cDNA; simple sequence repeat [SS...
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-01-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/18/2/1844 |
| _version_ | 1818922654758862848 |
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| author | Jong-Wook Chung Hyung-Jin Baek Sok-Young Lee Ho-Sun Lee Gyu-Taek Cho Jong-Hyun Park Sundan Suresh |
| author_facet | Jong-Wook Chung Hyung-Jin Baek Sok-Young Lee Ho-Sun Lee Gyu-Taek Cho Jong-Hyun Park Sundan Suresh |
| author_sort | Jong-Wook Chung |
| collection | DOAJ |
| description | Faba bean (Vicia faba L.) is a major food source and fodder legume, popularly known for its high content of seed-protein. Its role is critical in crop rotation, and for fixing nitrogen effectively. Polymorphic simple sequence repeat markers from transcript sequences (cDNA; simple sequence repeat [SSR]) were developed for faba bean (Vicia faba). We found that 1,729 SSR loci from 81,333 individual sequence reads and 240 primer pairs were designed and synthesized. In total, 55 primer pairs were found to be polymorphic and scorable consistently when screened in 32 accessions. The number of alleles ranged from 2 to 15, frequency of major alleles per locus varied from 0.17 to 0.91, the genotypes number ranged from 2 to 17, observed and expected heterozycosity values ranged from 0.00 to 0.44 and 0.17 to 0.89 and overall PIC values ranged from 0.16 to 0.88 respectively. These markers will be a useful tool for assessing the genetic diversity, understanding the population structure, and breeding patterns of faba bean. |
| first_indexed | 2024-12-20T01:56:59Z |
| format | Article |
| id | doaj.art-93251156d7b7456b879810b6fd295bdf |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-20T01:56:59Z |
| publishDate | 2013-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-93251156d7b7456b879810b6fd295bdf2022-12-21T19:57:27ZengMDPI AGMolecules1420-30492013-01-011821844185610.3390/molecules18021844Development and Molecular Characterization of 55 Novel Polymorphic cDNA-SSR Markers in Faba Bean (Vicia faba L.) Using 454 PyrosequencingJong-Wook ChungHyung-Jin BaekSok-Young LeeHo-Sun LeeGyu-Taek ChoJong-Hyun ParkSundan SureshFaba bean (Vicia faba L.) is a major food source and fodder legume, popularly known for its high content of seed-protein. Its role is critical in crop rotation, and for fixing nitrogen effectively. Polymorphic simple sequence repeat markers from transcript sequences (cDNA; simple sequence repeat [SSR]) were developed for faba bean (Vicia faba). We found that 1,729 SSR loci from 81,333 individual sequence reads and 240 primer pairs were designed and synthesized. In total, 55 primer pairs were found to be polymorphic and scorable consistently when screened in 32 accessions. The number of alleles ranged from 2 to 15, frequency of major alleles per locus varied from 0.17 to 0.91, the genotypes number ranged from 2 to 17, observed and expected heterozycosity values ranged from 0.00 to 0.44 and 0.17 to 0.89 and overall PIC values ranged from 0.16 to 0.88 respectively. These markers will be a useful tool for assessing the genetic diversity, understanding the population structure, and breeding patterns of faba bean.http://www.mdpi.com/1420-3049/18/2/1844cDNA-SSRgenetic diversity454 sequencingVicia faba |
| spellingShingle | Jong-Wook Chung Hyung-Jin Baek Sok-Young Lee Ho-Sun Lee Gyu-Taek Cho Jong-Hyun Park Sundan Suresh Development and Molecular Characterization of 55 Novel Polymorphic cDNA-SSR Markers in Faba Bean (Vicia faba L.) Using 454 Pyrosequencing Molecules cDNA-SSR genetic diversity 454 sequencing Vicia faba |
| title | Development and Molecular Characterization of 55 Novel Polymorphic cDNA-SSR Markers in Faba Bean (Vicia faba L.) Using 454 Pyrosequencing |
| title_full | Development and Molecular Characterization of 55 Novel Polymorphic cDNA-SSR Markers in Faba Bean (Vicia faba L.) Using 454 Pyrosequencing |
| title_fullStr | Development and Molecular Characterization of 55 Novel Polymorphic cDNA-SSR Markers in Faba Bean (Vicia faba L.) Using 454 Pyrosequencing |
| title_full_unstemmed | Development and Molecular Characterization of 55 Novel Polymorphic cDNA-SSR Markers in Faba Bean (Vicia faba L.) Using 454 Pyrosequencing |
| title_short | Development and Molecular Characterization of 55 Novel Polymorphic cDNA-SSR Markers in Faba Bean (Vicia faba L.) Using 454 Pyrosequencing |
| title_sort | development and molecular characterization of 55 novel polymorphic cdna ssr markers in faba bean vicia faba l using 454 pyrosequencing |
| topic | cDNA-SSR genetic diversity 454 sequencing Vicia faba |
| url | http://www.mdpi.com/1420-3049/18/2/1844 |
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