Current approaches to development of real-time qPCR test-kits
Currently fluorescent quantitative real-time polymerase chain reaction, which is a cutting-edge technology in genetic diagnosis, is used in different areas of molecularbiology. Practicaladvantageofsimplicityaswellascombination of high speed, sensitivityandspecificitymadeitpossibletousethisanalysisfo...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Da Vinci Media
2023-09-01
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| Series: | Ветеринария сегодня |
| Subjects: | |
| Online Access: | https://veterinary.arriah.ru/jour/article/view/739 |
| Summary: | Currently fluorescent quantitative real-time polymerase chain reaction, which is a cutting-edge technology in genetic diagnosis, is used in different areas of molecularbiology. Practicaladvantageofsimplicityaswellascombination of high speed, sensitivityandspecificitymadeitpossibletousethisanalysisfor nucleic acidquantitation. Thepaperpresentsgeneral information andrecommendedrules for thedevelopmentof real-timeqPCR. Thepublication isaimedtoacquaint theresearchersandreviewerswith necessaryrequirementstobefollowedin order toensure high accuracy, reliabilityandtransparencyof theexperiments, correct interpretation andrepeatabilityof thetest results. Currentapproachesaredescribedthatallow obtainingreliableandconsistent resultsbydifferentoperators, at differenttimesandin differentlaboratories. Basicrequirementsforreagentsused, nucleotidesequencesandvalidationmethodsaregiven. In general, thepublication givestheinformation neededtoachievethreeultimategoals: toprovidetheauthorswith abroadrangeoftoolsandrequirementsforthedevelopmentof real-time qPCR based-techniques; togivethepossibilitytothereviewersandeditorsofassessingthequalityofarticlesandguidelines/instructions in accordance with the requiredcriteria; toobtain consistentandreliableresultsof testsperformedusingthis method. |
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| ISSN: | 2304-196X 2658-6959 |