Current approaches to development of real-time qPCR test-kits
Currently fluorescent quantitative real-time polymerase chain reaction, which is a cutting-edge technology in genetic diagnosis, is used in different areas of molecularbiology. Practicaladvantageofsimplicityaswellascombination of high speed, sensitivityandspecificitymadeitpossibletousethisanalysisfo...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Da Vinci Media
2023-09-01
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| Series: | Ветеринария сегодня |
| Subjects: | |
| Online Access: | https://veterinary.arriah.ru/jour/article/view/739 |
| _version_ | 1797224819300761600 |
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| author | M. I. Doronin D. V. Mikhalishin A. V. Sprygin A. Mazloum T. V. Zhbanova K. N. Gruzdev E. V. Chernyshova |
| author_facet | M. I. Doronin D. V. Mikhalishin A. V. Sprygin A. Mazloum T. V. Zhbanova K. N. Gruzdev E. V. Chernyshova |
| author_sort | M. I. Doronin |
| collection | DOAJ |
| description | Currently fluorescent quantitative real-time polymerase chain reaction, which is a cutting-edge technology in genetic diagnosis, is used in different areas of molecularbiology. Practicaladvantageofsimplicityaswellascombination of high speed, sensitivityandspecificitymadeitpossibletousethisanalysisfor nucleic acidquantitation. Thepaperpresentsgeneral information andrecommendedrules for thedevelopmentof real-timeqPCR. Thepublication isaimedtoacquaint theresearchersandreviewerswith necessaryrequirementstobefollowedin order toensure high accuracy, reliabilityandtransparencyof theexperiments, correct interpretation andrepeatabilityof thetest results. Currentapproachesaredescribedthatallow obtainingreliableandconsistent resultsbydifferentoperators, at differenttimesandin differentlaboratories. Basicrequirementsforreagentsused, nucleotidesequencesandvalidationmethodsaregiven. In general, thepublication givestheinformation neededtoachievethreeultimategoals: toprovidetheauthorswith abroadrangeoftoolsandrequirementsforthedevelopmentof real-time qPCR based-techniques; togivethepossibilitytothereviewersandeditorsofassessingthequalityofarticlesandguidelines/instructions in accordance with the requiredcriteria; toobtain consistentandreliableresultsof testsperformedusingthis method. |
| first_indexed | 2024-03-09T02:48:24Z |
| format | Article |
| id | doaj.art-9325348fbbdf4d968e0b414c79dc5642 |
| institution | Directory Open Access Journal |
| issn | 2304-196X 2658-6959 |
| language | English |
| last_indexed | 2024-04-24T13:59:10Z |
| publishDate | 2023-09-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj.art-9325348fbbdf4d968e0b414c79dc56422024-04-03T12:48:51ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592023-09-0112319720710.29326/2304-196X-2023-12-3-197-207622Current approaches to development of real-time qPCR test-kitsM. I. Doronin0D. V. Mikhalishin1A. V. Sprygin2A. Mazloum3T. V. Zhbanova4K. N. Gruzdev5E. V. Chernyshova6FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)Currently fluorescent quantitative real-time polymerase chain reaction, which is a cutting-edge technology in genetic diagnosis, is used in different areas of molecularbiology. Practicaladvantageofsimplicityaswellascombination of high speed, sensitivityandspecificitymadeitpossibletousethisanalysisfor nucleic acidquantitation. Thepaperpresentsgeneral information andrecommendedrules for thedevelopmentof real-timeqPCR. Thepublication isaimedtoacquaint theresearchersandreviewerswith necessaryrequirementstobefollowedin order toensure high accuracy, reliabilityandtransparencyof theexperiments, correct interpretation andrepeatabilityof thetest results. Currentapproachesaredescribedthatallow obtainingreliableandconsistent resultsbydifferentoperators, at differenttimesandin differentlaboratories. Basicrequirementsforreagentsused, nucleotidesequencesandvalidationmethodsaregiven. In general, thepublication givestheinformation neededtoachievethreeultimategoals: toprovidetheauthorswith abroadrangeoftoolsandrequirementsforthedevelopmentof real-time qPCR based-techniques; togivethepossibilitytothereviewersandeditorsofassessingthequalityofarticlesandguidelines/instructions in accordance with the requiredcriteria; toobtain consistentandreliableresultsof testsperformedusingthis method.https://veterinary.arriah.ru/jour/article/view/739reviewreal-time polymerase chain reactionreverse transcriptionrequirements for test-kitsvalidationoligonucleotide primers and probesperformance of amplification reaction |
| spellingShingle | M. I. Doronin D. V. Mikhalishin A. V. Sprygin A. Mazloum T. V. Zhbanova K. N. Gruzdev E. V. Chernyshova Current approaches to development of real-time qPCR test-kits Ветеринария сегодня review real-time polymerase chain reaction reverse transcription requirements for test-kits validation oligonucleotide primers and probes performance of amplification reaction |
| title | Current approaches to development of real-time qPCR test-kits |
| title_full | Current approaches to development of real-time qPCR test-kits |
| title_fullStr | Current approaches to development of real-time qPCR test-kits |
| title_full_unstemmed | Current approaches to development of real-time qPCR test-kits |
| title_short | Current approaches to development of real-time qPCR test-kits |
| title_sort | current approaches to development of real time qpcr test kits |
| topic | review real-time polymerase chain reaction reverse transcription requirements for test-kits validation oligonucleotide primers and probes performance of amplification reaction |
| url | https://veterinary.arriah.ru/jour/article/view/739 |
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