Diversity of Acinetobacter baumannii in four French military hospitals, as assessed by multiple locus variable number of tandem repeats analysis.
<h4>Background</h4>Infections by A. calcoaceticus-A. baumannii (ACB) complex isolates represent a serious threat for wounded and burn patients. Three international multidrug-resistant (MDR) clones (EU clone I-III) are responsible for a large proportion of nosocomial infections with A. ba...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22984530/pdf/?tool=EBI |
| _version_ | 1818337520251830272 |
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| author | Yolande Hauck Charles Soler Patrick Jault Audrey Mérens Patrick Gérome Christine Mac Nab François Trueba Laurent Bargues Hoang Vu Thien Gilles Vergnaud Christine Pourcel |
| author_facet | Yolande Hauck Charles Soler Patrick Jault Audrey Mérens Patrick Gérome Christine Mac Nab François Trueba Laurent Bargues Hoang Vu Thien Gilles Vergnaud Christine Pourcel |
| author_sort | Yolande Hauck |
| collection | DOAJ |
| description | <h4>Background</h4>Infections by A. calcoaceticus-A. baumannii (ACB) complex isolates represent a serious threat for wounded and burn patients. Three international multidrug-resistant (MDR) clones (EU clone I-III) are responsible for a large proportion of nosocomial infections with A. baumannii but other emerging strains with high epidemic potential also occur.<h4>Methodology/principal findings</h4>We automatized a Multiple locus variable number of tandem repeats (VNTR) analysis (MLVA) protocol and used it to investigate the genetic diversity of 136 ACB isolates from four military hospitals and one childrens hospital. Acinetobacter sp other than baumannii isolates represented 22.6% (31/137) with a majority being A. pittii. The genotyping protocol designed for A.baumannii was also efficient to cluster A. pittii isolates. Fifty-five percent of A. baumannii isolates belonged to the two international clones I and II, and we identified new clones which members were found in the different hospitals. Analysis of two CRISPR-cas systems helped define two clonal complexes and provided phylogenetic information to help trace back their emergence.<h4>Conclusions/significance</h4>The increasing occurrence of A. baumannii infections in the hospital calls for measures to rapidly characterize the isolates and identify emerging clones. The automatized MLVA protocol can be the instrument for such surveys. In addition, the investigation of CRISPR/cas systems may give important keys to understand the evolution of some highly successful clonal complexes. |
| first_indexed | 2024-12-13T14:56:31Z |
| format | Article |
| id | doaj.art-9327f94584464b02acb43f7944054e96 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T14:56:31Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9327f94584464b02acb43f7944054e962022-12-21T23:41:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4459710.1371/journal.pone.0044597Diversity of Acinetobacter baumannii in four French military hospitals, as assessed by multiple locus variable number of tandem repeats analysis.Yolande HauckCharles SolerPatrick JaultAudrey MérensPatrick GéromeChristine Mac NabFrançois TruebaLaurent BarguesHoang Vu ThienGilles VergnaudChristine Pourcel<h4>Background</h4>Infections by A. calcoaceticus-A. baumannii (ACB) complex isolates represent a serious threat for wounded and burn patients. Three international multidrug-resistant (MDR) clones (EU clone I-III) are responsible for a large proportion of nosocomial infections with A. baumannii but other emerging strains with high epidemic potential also occur.<h4>Methodology/principal findings</h4>We automatized a Multiple locus variable number of tandem repeats (VNTR) analysis (MLVA) protocol and used it to investigate the genetic diversity of 136 ACB isolates from four military hospitals and one childrens hospital. Acinetobacter sp other than baumannii isolates represented 22.6% (31/137) with a majority being A. pittii. The genotyping protocol designed for A.baumannii was also efficient to cluster A. pittii isolates. Fifty-five percent of A. baumannii isolates belonged to the two international clones I and II, and we identified new clones which members were found in the different hospitals. Analysis of two CRISPR-cas systems helped define two clonal complexes and provided phylogenetic information to help trace back their emergence.<h4>Conclusions/significance</h4>The increasing occurrence of A. baumannii infections in the hospital calls for measures to rapidly characterize the isolates and identify emerging clones. The automatized MLVA protocol can be the instrument for such surveys. In addition, the investigation of CRISPR/cas systems may give important keys to understand the evolution of some highly successful clonal complexes.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22984530/pdf/?tool=EBI |
| spellingShingle | Yolande Hauck Charles Soler Patrick Jault Audrey Mérens Patrick Gérome Christine Mac Nab François Trueba Laurent Bargues Hoang Vu Thien Gilles Vergnaud Christine Pourcel Diversity of Acinetobacter baumannii in four French military hospitals, as assessed by multiple locus variable number of tandem repeats analysis. PLoS ONE |
| title | Diversity of Acinetobacter baumannii in four French military hospitals, as assessed by multiple locus variable number of tandem repeats analysis. |
| title_full | Diversity of Acinetobacter baumannii in four French military hospitals, as assessed by multiple locus variable number of tandem repeats analysis. |
| title_fullStr | Diversity of Acinetobacter baumannii in four French military hospitals, as assessed by multiple locus variable number of tandem repeats analysis. |
| title_full_unstemmed | Diversity of Acinetobacter baumannii in four French military hospitals, as assessed by multiple locus variable number of tandem repeats analysis. |
| title_short | Diversity of Acinetobacter baumannii in four French military hospitals, as assessed by multiple locus variable number of tandem repeats analysis. |
| title_sort | diversity of acinetobacter baumannii in four french military hospitals as assessed by multiple locus variable number of tandem repeats analysis |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22984530/pdf/?tool=EBI |
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