oqxAB-Positive IncHI2 Plasmid pHXY0908 Increase Salmonella enterica Serotype Typhimurium Strains Tolerance to Ciprofloxacin
Salmonella enterica serotype Typhimurium is a major global food-borne pathogen and causes life-threatening infections. Although the resistance mechanisms to fluoroquinolones in S. Typhimurium had been well-defined, tolerance to fluoroquinolones and the associated mechanism for this are obscure. In t...
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| Format: | Article |
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Frontiers Media S.A.
2019-07-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fcimb.2019.00242/full |
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| author | Xinlei Lian Xinlei Lian Xinlei Lian Xiran Wang Xiran Wang Xiran Wang Xiao Liu Xiao Liu Xiao Liu Jing Xia Jing Xia Jing Xia Liangxing Fang Liangxing Fang Liangxing Fang Jian Sun Jian Sun Jian Sun Xiaoping Liao Xiaoping Liao Xiaoping Liao Yahong Liu Yahong Liu Yahong Liu |
| author_facet | Xinlei Lian Xinlei Lian Xinlei Lian Xiran Wang Xiran Wang Xiran Wang Xiao Liu Xiao Liu Xiao Liu Jing Xia Jing Xia Jing Xia Liangxing Fang Liangxing Fang Liangxing Fang Jian Sun Jian Sun Jian Sun Xiaoping Liao Xiaoping Liao Xiaoping Liao Yahong Liu Yahong Liu Yahong Liu |
| author_sort | Xinlei Lian |
| collection | DOAJ |
| description | Salmonella enterica serotype Typhimurium is a major global food-borne pathogen and causes life-threatening infections. Although the resistance mechanisms to fluoroquinolones in S. Typhimurium had been well-defined, tolerance to fluoroquinolones and the associated mechanism for this are obscure. In the current work, we investigated an oqxAB-positive plasmid pHXY0908 and analyzed its role in S. Typhimurium tolerance to ciprofloxacin using time-kill, transcriptome sequencing and real-time PCR. S. Typhimurium ATCC14028 could survive under lethal concentrations of ciprofloxacin after acquiring plasmid pHXY0908. Transcriptome sequence analysis showed the chromosomal genes were systematically regulated after acquiring this plasmid suggesting an interaction between chromosome and plasmid. Additionally, the chromosomal efflux pump genes acrB, acrA, tolC, and yceE were up-regulated after acquiring plasmid pHXY0908 suggesting that these efflux pumps may contribute to the survival of ATCC14028 exposed to the lethal concentrations of ciprofloxacin. In conclusion, this is the first known report demonstrating that an IncHI2 type plasmid harboring oqxAB could assist S. Typhimurium survival under lethal concentrations of ciprofloxacin. |
| first_indexed | 2024-12-13T08:20:20Z |
| format | Article |
| id | doaj.art-9328e74d02004f40af826ec73502bb82 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-13T08:20:20Z |
| publishDate | 2019-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-9328e74d02004f40af826ec73502bb822022-12-21T23:54:02ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882019-07-01910.3389/fcimb.2019.00242462092oqxAB-Positive IncHI2 Plasmid pHXY0908 Increase Salmonella enterica Serotype Typhimurium Strains Tolerance to CiprofloxacinXinlei Lian0Xinlei Lian1Xinlei Lian2Xiran Wang3Xiran Wang4Xiran Wang5Xiao Liu6Xiao Liu7Xiao Liu8Jing Xia9Jing Xia10Jing Xia11Liangxing Fang12Liangxing Fang13Liangxing Fang14Jian Sun15Jian Sun16Jian Sun17Xiaoping Liao18Xiaoping Liao19Xiaoping Liao20Yahong Liu21Yahong Liu22Yahong Liu23National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, ChinaLaboratory of Veterinary Pharmacology, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, ChinaNational Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, ChinaLaboratory of Veterinary Pharmacology, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, ChinaNational Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, ChinaLaboratory of Veterinary Pharmacology, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, ChinaNational Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, ChinaLaboratory of Veterinary Pharmacology, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, ChinaNational Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, ChinaLaboratory of Veterinary Pharmacology, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, ChinaNational Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, ChinaLaboratory of Veterinary Pharmacology, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, ChinaNational Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, ChinaLaboratory of Veterinary Pharmacology, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, ChinaNational Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, ChinaLaboratory of Veterinary Pharmacology, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, ChinaSalmonella enterica serotype Typhimurium is a major global food-borne pathogen and causes life-threatening infections. Although the resistance mechanisms to fluoroquinolones in S. Typhimurium had been well-defined, tolerance to fluoroquinolones and the associated mechanism for this are obscure. In the current work, we investigated an oqxAB-positive plasmid pHXY0908 and analyzed its role in S. Typhimurium tolerance to ciprofloxacin using time-kill, transcriptome sequencing and real-time PCR. S. Typhimurium ATCC14028 could survive under lethal concentrations of ciprofloxacin after acquiring plasmid pHXY0908. Transcriptome sequence analysis showed the chromosomal genes were systematically regulated after acquiring this plasmid suggesting an interaction between chromosome and plasmid. Additionally, the chromosomal efflux pump genes acrB, acrA, tolC, and yceE were up-regulated after acquiring plasmid pHXY0908 suggesting that these efflux pumps may contribute to the survival of ATCC14028 exposed to the lethal concentrations of ciprofloxacin. In conclusion, this is the first known report demonstrating that an IncHI2 type plasmid harboring oqxAB could assist S. Typhimurium survival under lethal concentrations of ciprofloxacin.https://www.frontiersin.org/article/10.3389/fcimb.2019.00242/fulloqxABIncHI2plasmidsSalmonellatolerance |
| spellingShingle | Xinlei Lian Xinlei Lian Xinlei Lian Xiran Wang Xiran Wang Xiran Wang Xiao Liu Xiao Liu Xiao Liu Jing Xia Jing Xia Jing Xia Liangxing Fang Liangxing Fang Liangxing Fang Jian Sun Jian Sun Jian Sun Xiaoping Liao Xiaoping Liao Xiaoping Liao Yahong Liu Yahong Liu Yahong Liu oqxAB-Positive IncHI2 Plasmid pHXY0908 Increase Salmonella enterica Serotype Typhimurium Strains Tolerance to Ciprofloxacin Frontiers in Cellular and Infection Microbiology oqxAB IncHI2 plasmids Salmonella tolerance |
| title | oqxAB-Positive IncHI2 Plasmid pHXY0908 Increase Salmonella enterica Serotype Typhimurium Strains Tolerance to Ciprofloxacin |
| title_full | oqxAB-Positive IncHI2 Plasmid pHXY0908 Increase Salmonella enterica Serotype Typhimurium Strains Tolerance to Ciprofloxacin |
| title_fullStr | oqxAB-Positive IncHI2 Plasmid pHXY0908 Increase Salmonella enterica Serotype Typhimurium Strains Tolerance to Ciprofloxacin |
| title_full_unstemmed | oqxAB-Positive IncHI2 Plasmid pHXY0908 Increase Salmonella enterica Serotype Typhimurium Strains Tolerance to Ciprofloxacin |
| title_short | oqxAB-Positive IncHI2 Plasmid pHXY0908 Increase Salmonella enterica Serotype Typhimurium Strains Tolerance to Ciprofloxacin |
| title_sort | oqxab positive inchi2 plasmid phxy0908 increase salmonella enterica serotype typhimurium strains tolerance to ciprofloxacin |
| topic | oqxAB IncHI2 plasmids Salmonella tolerance |
| url | https://www.frontiersin.org/article/10.3389/fcimb.2019.00242/full |
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