Hybrid Nanoparticle-Assisted Chemo-Photothermal Therapy and Photoacoustic Imaging in a Three-Dimensional Breast Cancer Cell Model
Bioinspired nanoparticles have recently been gaining attention as promising multifunctional nanoplatforms for therapeutic applications in cancer, including breast cancer. Here, the efficiency of the chemo-photothermal and photoacoustic properties of hybrid albumin-modified nanoparticles (HSA-NPs) lo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/24/17374 |
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| author | Barbara Carrese Chiara Cavallini Paolo Armanetti Brigida Silvestri Gaetano Calì Giuseppina Luciani Gennaro Sanità Luca Menichetti Annalisa Lamberti |
| author_facet | Barbara Carrese Chiara Cavallini Paolo Armanetti Brigida Silvestri Gaetano Calì Giuseppina Luciani Gennaro Sanità Luca Menichetti Annalisa Lamberti |
| author_sort | Barbara Carrese |
| collection | DOAJ |
| description | Bioinspired nanoparticles have recently been gaining attention as promising multifunctional nanoplatforms for therapeutic applications in cancer, including breast cancer. Here, the efficiency of the chemo-photothermal and photoacoustic properties of hybrid albumin-modified nanoparticles (HSA-NPs) loaded with doxorubicin was evaluated in a three-dimensional breast cancer cell model. The HSA-NPs showed a higher uptake and deeper penetration into breast cancer spheroids than healthy breast cell 3D cultures. Confocal microscopy revealed that, in tumour spheroids incubated with doxorubicin-loaded NPs for 16 h, doxorubicin was mainly localised in the cytoplasm, while a strong signal was detectable at the nuclear level after 24 h, suggesting a time-dependent uptake. To evaluate the cytotoxicity of doxorubicin-loaded NPs, tumour spheroids were treated for up to 96 h with increasing concentrations of NPs, showing marked toxicity only at the highest concentration of doxorubicin. When doxorubicin administration was combined with laser photothermal irradiation, enhanced cytotoxicity was observed at lower concentrations and incubation times. Finally, the photoacoustic properties of doxorubicin-loaded NPs were evaluated in tumour spheroids, showing a detectable signal increasing with NP concentration. Overall, our data show that the combined effect of chemo-photothermal therapy results in a shorter exposure time to doxorubicin and a lower drug dose. Furthermore, owing to the photoacoustic properties of the NPs, this nanoplatform may represent a good candidate for theranostic applications. |
| first_indexed | 2024-03-08T20:41:58Z |
| format | Article |
| id | doaj.art-932b8260e995414b9da8131a9f25698f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T20:41:58Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-932b8260e995414b9da8131a9f25698f2023-12-22T14:14:14ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0124241737410.3390/ijms242417374Hybrid Nanoparticle-Assisted Chemo-Photothermal Therapy and Photoacoustic Imaging in a Three-Dimensional Breast Cancer Cell ModelBarbara Carrese0Chiara Cavallini1Paolo Armanetti2Brigida Silvestri3Gaetano Calì4Giuseppina Luciani5Gennaro Sanità6Luca Menichetti7Annalisa Lamberti8Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 80131 Naples, ItalyInstitute of Clinical Physiology, National Research Council, 56124 Pisa, ItalyInstitute of Clinical Physiology, National Research Council, 56124 Pisa, ItalyDepartment of Civil, Construction and Environmental Engineering, University of Naples Federico II, 80125 Naples, ItalyInstitute of Endocrinology and Molecular Oncology, National Research Council, 80131 Naples, ItalyDepartment of Chemical, Materials and Production Engineering, University of Naples Federico II, 80125 Naples, ItalyInstitute of Applied Sciences and Intelligent Systems—Unit of Naples, National Research Council, 80131 Naples, ItalyInstitute of Clinical Physiology, National Research Council, 56124 Pisa, ItalyDepartment of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 80131 Naples, ItalyBioinspired nanoparticles have recently been gaining attention as promising multifunctional nanoplatforms for therapeutic applications in cancer, including breast cancer. Here, the efficiency of the chemo-photothermal and photoacoustic properties of hybrid albumin-modified nanoparticles (HSA-NPs) loaded with doxorubicin was evaluated in a three-dimensional breast cancer cell model. The HSA-NPs showed a higher uptake and deeper penetration into breast cancer spheroids than healthy breast cell 3D cultures. Confocal microscopy revealed that, in tumour spheroids incubated with doxorubicin-loaded NPs for 16 h, doxorubicin was mainly localised in the cytoplasm, while a strong signal was detectable at the nuclear level after 24 h, suggesting a time-dependent uptake. To evaluate the cytotoxicity of doxorubicin-loaded NPs, tumour spheroids were treated for up to 96 h with increasing concentrations of NPs, showing marked toxicity only at the highest concentration of doxorubicin. When doxorubicin administration was combined with laser photothermal irradiation, enhanced cytotoxicity was observed at lower concentrations and incubation times. Finally, the photoacoustic properties of doxorubicin-loaded NPs were evaluated in tumour spheroids, showing a detectable signal increasing with NP concentration. Overall, our data show that the combined effect of chemo-photothermal therapy results in a shorter exposure time to doxorubicin and a lower drug dose. Furthermore, owing to the photoacoustic properties of the NPs, this nanoplatform may represent a good candidate for theranostic applications.https://www.mdpi.com/1422-0067/24/24/17374albumin-modified nanoparticlesmelanindoxorubicinbreast cancer spheroidsphotoacoustic imagingphotothermal therapy |
| spellingShingle | Barbara Carrese Chiara Cavallini Paolo Armanetti Brigida Silvestri Gaetano Calì Giuseppina Luciani Gennaro Sanità Luca Menichetti Annalisa Lamberti Hybrid Nanoparticle-Assisted Chemo-Photothermal Therapy and Photoacoustic Imaging in a Three-Dimensional Breast Cancer Cell Model International Journal of Molecular Sciences albumin-modified nanoparticles melanin doxorubicin breast cancer spheroids photoacoustic imaging photothermal therapy |
| title | Hybrid Nanoparticle-Assisted Chemo-Photothermal Therapy and Photoacoustic Imaging in a Three-Dimensional Breast Cancer Cell Model |
| title_full | Hybrid Nanoparticle-Assisted Chemo-Photothermal Therapy and Photoacoustic Imaging in a Three-Dimensional Breast Cancer Cell Model |
| title_fullStr | Hybrid Nanoparticle-Assisted Chemo-Photothermal Therapy and Photoacoustic Imaging in a Three-Dimensional Breast Cancer Cell Model |
| title_full_unstemmed | Hybrid Nanoparticle-Assisted Chemo-Photothermal Therapy and Photoacoustic Imaging in a Three-Dimensional Breast Cancer Cell Model |
| title_short | Hybrid Nanoparticle-Assisted Chemo-Photothermal Therapy and Photoacoustic Imaging in a Three-Dimensional Breast Cancer Cell Model |
| title_sort | hybrid nanoparticle assisted chemo photothermal therapy and photoacoustic imaging in a three dimensional breast cancer cell model |
| topic | albumin-modified nanoparticles melanin doxorubicin breast cancer spheroids photoacoustic imaging photothermal therapy |
| url | https://www.mdpi.com/1422-0067/24/24/17374 |
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