Colonoscopy aspiration lavages for mucosal metataxonomic profiling of spondylarthritis-associated gastrointestinal tract alterations
Abstract The study of the GI-tract microbiota of spondylarthritis (SpA) patients has focused on the analysis of feces samples, that picture mostly the luminal microbiota. The aim of this study was to determine the contribution of mucosal and luminal microbiome to the gut dysbiosis in SpA, using colo...
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| Format: | Article |
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Nature Portfolio
2023-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-33597-y |
| _version_ | 1797836425999155200 |
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| author | Ricaurte A. Marquez-Ortiz Moises Leon Deisy Abril Javier Escobar-Perez Cristian Florez-Sarmiento Viviana Parra-Izquierdo Philippe Chalem Consuelo Romero-Sanchez |
| author_facet | Ricaurte A. Marquez-Ortiz Moises Leon Deisy Abril Javier Escobar-Perez Cristian Florez-Sarmiento Viviana Parra-Izquierdo Philippe Chalem Consuelo Romero-Sanchez |
| author_sort | Ricaurte A. Marquez-Ortiz |
| collection | DOAJ |
| description | Abstract The study of the GI-tract microbiota of spondylarthritis (SpA) patients has focused on the analysis of feces samples, that picture mostly the luminal microbiota. The aim of this study was to determine the contribution of mucosal and luminal microbiome to the gut dysbiosis in SpA, using colonoscopy aspiration lavages (CAL), a recent alternative for regional studies of the GI-tract. We analyzed 59 CAL (from sigmoid colon and distal ileum), and 41 feces samples, from 32 SpA patients and 7 healthy individuals, using 16S rRNA gene-targeted metataxonomic profiling. It was found high prevalence of GI-tract manifestations among SpA patients (65.3%). Metataxonomic profiling, confirmed CAL samples from the lower GI tract (colon or ileum) presented a distinctive and undifferentiated bacteriome and separate from that found in feces’ samples or in the beginning of the GI tract (oral cavity (OC)). Lower GI-tract samples and feces of SpA patients exhibited similar behavior to the microbiota of IBD group with reduced microbial richness and diversity, comparing to the healthy controls. Interestingly, it was found increase in proinflammatory taxa in SpA patients, such as Enterobacteriaceae family (mostly in the ileum), Succinivibrio spp. and Prevotella stercorea. Conversely, SpA patients presented significant decrease in the SCFA producers Coprococcus catus and Eubacterium biforme. Our data support the value of CAL samples for the regional study of GI-tract and contribute with information of potential “disruptor taxa” involved in the GI-tract associated disorders observed in SpA patients. |
| first_indexed | 2024-04-09T15:09:54Z |
| format | Article |
| id | doaj.art-932c0e5228d54c09a5e7501dc84c3d26 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T15:09:54Z |
| publishDate | 2023-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-932c0e5228d54c09a5e7501dc84c3d262023-04-30T11:16:42ZengNature PortfolioScientific Reports2045-23222023-04-0113111310.1038/s41598-023-33597-yColonoscopy aspiration lavages for mucosal metataxonomic profiling of spondylarthritis-associated gastrointestinal tract alterationsRicaurte A. Marquez-Ortiz0Moises Leon1Deisy Abril2Javier Escobar-Perez3Cristian Florez-Sarmiento4Viviana Parra-Izquierdo5Philippe Chalem6Consuelo Romero-Sanchez7Bacterial Molecular Genetics Laboratory/LGMB, Vicerrectoría de Investigaciones, Universidad El BosqueMaster’s Program in Basic Biomedical Sciences, Faculty of Science, Universidad El BosqueBacterial Molecular Genetics Laboratory/LGMB, Vicerrectoría de Investigaciones, Universidad El BosqueBacterial Molecular Genetics Laboratory/LGMB, Vicerrectoría de Investigaciones, Universidad El BosqueCellular and Molecular Immunology Group/INMUBO, School of Dentistry, Universidad El BosqueCellular and Molecular Immunology Group/INMUBO, School of Dentistry, Universidad El BosqueFundación Instituto de Reumatología Fernando ChalemCellular and Molecular Immunology Group/INMUBO, School of Dentistry, Universidad El BosqueAbstract The study of the GI-tract microbiota of spondylarthritis (SpA) patients has focused on the analysis of feces samples, that picture mostly the luminal microbiota. The aim of this study was to determine the contribution of mucosal and luminal microbiome to the gut dysbiosis in SpA, using colonoscopy aspiration lavages (CAL), a recent alternative for regional studies of the GI-tract. We analyzed 59 CAL (from sigmoid colon and distal ileum), and 41 feces samples, from 32 SpA patients and 7 healthy individuals, using 16S rRNA gene-targeted metataxonomic profiling. It was found high prevalence of GI-tract manifestations among SpA patients (65.3%). Metataxonomic profiling, confirmed CAL samples from the lower GI tract (colon or ileum) presented a distinctive and undifferentiated bacteriome and separate from that found in feces’ samples or in the beginning of the GI tract (oral cavity (OC)). Lower GI-tract samples and feces of SpA patients exhibited similar behavior to the microbiota of IBD group with reduced microbial richness and diversity, comparing to the healthy controls. Interestingly, it was found increase in proinflammatory taxa in SpA patients, such as Enterobacteriaceae family (mostly in the ileum), Succinivibrio spp. and Prevotella stercorea. Conversely, SpA patients presented significant decrease in the SCFA producers Coprococcus catus and Eubacterium biforme. Our data support the value of CAL samples for the regional study of GI-tract and contribute with information of potential “disruptor taxa” involved in the GI-tract associated disorders observed in SpA patients.https://doi.org/10.1038/s41598-023-33597-y |
| spellingShingle | Ricaurte A. Marquez-Ortiz Moises Leon Deisy Abril Javier Escobar-Perez Cristian Florez-Sarmiento Viviana Parra-Izquierdo Philippe Chalem Consuelo Romero-Sanchez Colonoscopy aspiration lavages for mucosal metataxonomic profiling of spondylarthritis-associated gastrointestinal tract alterations Scientific Reports |
| title | Colonoscopy aspiration lavages for mucosal metataxonomic profiling of spondylarthritis-associated gastrointestinal tract alterations |
| title_full | Colonoscopy aspiration lavages for mucosal metataxonomic profiling of spondylarthritis-associated gastrointestinal tract alterations |
| title_fullStr | Colonoscopy aspiration lavages for mucosal metataxonomic profiling of spondylarthritis-associated gastrointestinal tract alterations |
| title_full_unstemmed | Colonoscopy aspiration lavages for mucosal metataxonomic profiling of spondylarthritis-associated gastrointestinal tract alterations |
| title_short | Colonoscopy aspiration lavages for mucosal metataxonomic profiling of spondylarthritis-associated gastrointestinal tract alterations |
| title_sort | colonoscopy aspiration lavages for mucosal metataxonomic profiling of spondylarthritis associated gastrointestinal tract alterations |
| url | https://doi.org/10.1038/s41598-023-33597-y |
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