Impact and Diversity of ESBL-Producing <i>Klebsiella pneumoniae</i> Recovered from Raw Chicken Meat Samples in Türkiye
The interrelationship between human, animal and environmental sectors leads to the spread of antibiotic resistance due to selective pressures, evolutionary traits and genomic evolution. In particular, the frequent use of antibiotics in livestock inevitably influences the emergence of specific resist...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/13/1/14 |
| _version_ | 1797344759500505088 |
|---|---|
| author | Cemil Kürekci Özlem Ünaldı Seyda Şahin Isidro García-Meniño Jens Andre Hammerl |
| author_facet | Cemil Kürekci Özlem Ünaldı Seyda Şahin Isidro García-Meniño Jens Andre Hammerl |
| author_sort | Cemil Kürekci |
| collection | DOAJ |
| description | The interrelationship between human, animal and environmental sectors leads to the spread of antibiotic resistance due to selective pressures, evolutionary traits and genomic evolution. In particular, the frequent use of antibiotics in livestock inevitably influences the emergence of specific resistance determinants in human strains, associated with reduced treatment options in clinical therapy. In this study, ESBL-producing <i>Klebsiella pneumoniae</i> strains isolated from chicken meat samples were evaluated for public health implications in Türkiye. Whole-genome sequencing was used for genetic dissection and phylogenetic comparison of their genomes. The isolates were assigned to four MLST types (ST147, ST37, ST2747 and ST219); two of them were found to represent the ST147 clone associated with severe human infections worldwide. In addition to cephalosporins, high resistance levels to quinolones/fluoroquinolones were identified phenotypically, caused by acquired resistance genes and chromosomal point variations. One isolate was also found to carry the <i>qacE</i>∆1 efflux transporter gene, which confers tolerance to quaternary ammonium compounds. The detection of virulence genes (i.e., that coding for enterobactin) associated with the pathogenicity of <i>K. pneumoniae</i> suggests a public health impact. Thus, comprehensive information on the occurrence and impact of <i>K. pneumoniae</i> from livestock is needed to derive appropriate management strategies for consumer protection. In this study, it was shown that poultry meat serves as a reservoir of clinically emerging multidrug-resistant high-risk clones. |
| first_indexed | 2024-03-08T11:07:24Z |
| format | Article |
| id | doaj.art-932dd021dcfa4830908286e567aa7b88 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-08T11:07:24Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-932dd021dcfa4830908286e567aa7b882024-01-26T14:36:26ZengMDPI AGAntibiotics2079-63822023-12-011311410.3390/antibiotics13010014Impact and Diversity of ESBL-Producing <i>Klebsiella pneumoniae</i> Recovered from Raw Chicken Meat Samples in TürkiyeCemil Kürekci0Özlem Ünaldı1Seyda Şahin2Isidro García-Meniño3Jens Andre Hammerl4Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, Hatay 31060, TürkiyeDepartment of Microbiology Reference Laboratories, General Directorate of Public Health, Ministry of Health, Ankara 06430, TürkiyeDepartment of Food Hygiene and Technology, Faculty of Veterinary Medicine, Sivas Cumhuriyet University, Sivas 58070, TürkiyeLaboratorio de Referencia de <i>Escherichia coli</i> (LREC), Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela (USC), 27002 Lugo, SpainDepartment Biological Safety, German Federal Institute for Risk Assessment, 10589 Berlin, GermanyThe interrelationship between human, animal and environmental sectors leads to the spread of antibiotic resistance due to selective pressures, evolutionary traits and genomic evolution. In particular, the frequent use of antibiotics in livestock inevitably influences the emergence of specific resistance determinants in human strains, associated with reduced treatment options in clinical therapy. In this study, ESBL-producing <i>Klebsiella pneumoniae</i> strains isolated from chicken meat samples were evaluated for public health implications in Türkiye. Whole-genome sequencing was used for genetic dissection and phylogenetic comparison of their genomes. The isolates were assigned to four MLST types (ST147, ST37, ST2747 and ST219); two of them were found to represent the ST147 clone associated with severe human infections worldwide. In addition to cephalosporins, high resistance levels to quinolones/fluoroquinolones were identified phenotypically, caused by acquired resistance genes and chromosomal point variations. One isolate was also found to carry the <i>qacE</i>∆1 efflux transporter gene, which confers tolerance to quaternary ammonium compounds. The detection of virulence genes (i.e., that coding for enterobactin) associated with the pathogenicity of <i>K. pneumoniae</i> suggests a public health impact. Thus, comprehensive information on the occurrence and impact of <i>K. pneumoniae</i> from livestock is needed to derive appropriate management strategies for consumer protection. In this study, it was shown that poultry meat serves as a reservoir of clinically emerging multidrug-resistant high-risk clones.https://www.mdpi.com/2079-6382/13/1/14multidrug resistance<i>K. pneumoniae</i>One Healthfoodzoonosisrisk evaluation |
| spellingShingle | Cemil Kürekci Özlem Ünaldı Seyda Şahin Isidro García-Meniño Jens Andre Hammerl Impact and Diversity of ESBL-Producing <i>Klebsiella pneumoniae</i> Recovered from Raw Chicken Meat Samples in Türkiye Antibiotics multidrug resistance <i>K. pneumoniae</i> One Health food zoonosis risk evaluation |
| title | Impact and Diversity of ESBL-Producing <i>Klebsiella pneumoniae</i> Recovered from Raw Chicken Meat Samples in Türkiye |
| title_full | Impact and Diversity of ESBL-Producing <i>Klebsiella pneumoniae</i> Recovered from Raw Chicken Meat Samples in Türkiye |
| title_fullStr | Impact and Diversity of ESBL-Producing <i>Klebsiella pneumoniae</i> Recovered from Raw Chicken Meat Samples in Türkiye |
| title_full_unstemmed | Impact and Diversity of ESBL-Producing <i>Klebsiella pneumoniae</i> Recovered from Raw Chicken Meat Samples in Türkiye |
| title_short | Impact and Diversity of ESBL-Producing <i>Klebsiella pneumoniae</i> Recovered from Raw Chicken Meat Samples in Türkiye |
| title_sort | impact and diversity of esbl producing i klebsiella pneumoniae i recovered from raw chicken meat samples in turkiye |
| topic | multidrug resistance <i>K. pneumoniae</i> One Health food zoonosis risk evaluation |
| url | https://www.mdpi.com/2079-6382/13/1/14 |
| work_keys_str_mv | AT cemilkurekci impactanddiversityofesblproducingiklebsiellapneumoniaeirecoveredfromrawchickenmeatsamplesinturkiye AT ozlemunaldı impactanddiversityofesblproducingiklebsiellapneumoniaeirecoveredfromrawchickenmeatsamplesinturkiye AT seydasahin impactanddiversityofesblproducingiklebsiellapneumoniaeirecoveredfromrawchickenmeatsamplesinturkiye AT isidrogarciamenino impactanddiversityofesblproducingiklebsiellapneumoniaeirecoveredfromrawchickenmeatsamplesinturkiye AT jensandrehammerl impactanddiversityofesblproducingiklebsiellapneumoniaeirecoveredfromrawchickenmeatsamplesinturkiye |