High-Throughput Root Imaging Analysis Reveals Wide Variation in Root Morphology of Wild Adzuki bean (<i>Vigna angularis</i>) Accessions
Root system architecture and morphological diversification in wild accessions are important for crop improvement and productivity in adzuki beans. In this study, via analysis using 2-dimensional (2D) root imaging and WinRHIZO Pro software, we described the root traits of 61 adzuki bean accessions in...
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MDPI AG
2022-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/3/405 |
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| author | Rupesh Tayade Seong-Hoon Kim Pooja Tripathi Yi-Dam Choi Jung-Beom Yoon Yoon-Ha Kim |
| author_facet | Rupesh Tayade Seong-Hoon Kim Pooja Tripathi Yi-Dam Choi Jung-Beom Yoon Yoon-Ha Kim |
| author_sort | Rupesh Tayade |
| collection | DOAJ |
| description | Root system architecture and morphological diversification in wild accessions are important for crop improvement and productivity in adzuki beans. In this study, via analysis using 2-dimensional (2D) root imaging and WinRHIZO Pro software, we described the root traits of 61 adzuki bean accessions in their early vegetative growth stage. These accessions were chosen for study because they are used in Korea’s crop improvement programs; however, their root traits have not been sufficiently investigated. Analysis of variance revealed a significant difference between the accessions of all measured root traits. Distribution analysis demonstrated that most of the root traits followed normal distribution. The accessions showed up to a 17-fold increase in the values in contrasting accessions for the root traits. For total root length (TRL), the values ranged from 82.43 to 1435 cm, and for surface area (SA), they ranged from 12.30 to 208.39 cm<sup>2</sup>. The values for average diameter (AD) ranged from 0.23 to 0.56 mm. Significant differences were observed for other traits. Overall, the results showed that the accession IT 305544 had the highest TRL, SA, and number of tips (NT), whereas IT 262477 and IT 262492 showed the lowest values for TRL, SA, and AD. Principal component analysis showed an 89% variance for PC1 and PC2. K-mean clustering explained 77.4% of the variance in the data and grouped the accessions into three clusters. All six root traits had greater coefficients of variation (≥15%) among the tested accessions. Furthermore, to determine which root traits best distinguished different accessions, the correlation within our set of accessions provided trait-based ranking depending on their contribution. The identified accessions may be advantageous for the development of new crossing combinations to improve root features in adzuki beans during the early growth stage. The root traits assessed in this study could be attributes for future adzuki bean crop selection and improvement. |
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| language | English |
| last_indexed | 2024-03-09T23:18:43Z |
| publishDate | 2022-02-01 |
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| series | Plants |
| spelling | doaj.art-933048a727d14861ac64a381839183602023-11-23T17:31:27ZengMDPI AGPlants2223-77472022-02-0111340510.3390/plants11030405High-Throughput Root Imaging Analysis Reveals Wide Variation in Root Morphology of Wild Adzuki bean (<i>Vigna angularis</i>) AccessionsRupesh Tayade0Seong-Hoon Kim1Pooja Tripathi2Yi-Dam Choi3Jung-Beom Yoon4Yoon-Ha Kim5Department of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaNational Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju 54874, KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaHorticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, RDA, Jeonju 54874, KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaRoot system architecture and morphological diversification in wild accessions are important for crop improvement and productivity in adzuki beans. In this study, via analysis using 2-dimensional (2D) root imaging and WinRHIZO Pro software, we described the root traits of 61 adzuki bean accessions in their early vegetative growth stage. These accessions were chosen for study because they are used in Korea’s crop improvement programs; however, their root traits have not been sufficiently investigated. Analysis of variance revealed a significant difference between the accessions of all measured root traits. Distribution analysis demonstrated that most of the root traits followed normal distribution. The accessions showed up to a 17-fold increase in the values in contrasting accessions for the root traits. For total root length (TRL), the values ranged from 82.43 to 1435 cm, and for surface area (SA), they ranged from 12.30 to 208.39 cm<sup>2</sup>. The values for average diameter (AD) ranged from 0.23 to 0.56 mm. Significant differences were observed for other traits. Overall, the results showed that the accession IT 305544 had the highest TRL, SA, and number of tips (NT), whereas IT 262477 and IT 262492 showed the lowest values for TRL, SA, and AD. Principal component analysis showed an 89% variance for PC1 and PC2. K-mean clustering explained 77.4% of the variance in the data and grouped the accessions into three clusters. All six root traits had greater coefficients of variation (≥15%) among the tested accessions. Furthermore, to determine which root traits best distinguished different accessions, the correlation within our set of accessions provided trait-based ranking depending on their contribution. The identified accessions may be advantageous for the development of new crossing combinations to improve root features in adzuki beans during the early growth stage. The root traits assessed in this study could be attributes for future adzuki bean crop selection and improvement.https://www.mdpi.com/2223-7747/11/3/405cluster analysisimage analysislegumeroot architectural traitroot morphological trait |
| spellingShingle | Rupesh Tayade Seong-Hoon Kim Pooja Tripathi Yi-Dam Choi Jung-Beom Yoon Yoon-Ha Kim High-Throughput Root Imaging Analysis Reveals Wide Variation in Root Morphology of Wild Adzuki bean (<i>Vigna angularis</i>) Accessions Plants cluster analysis image analysis legume root architectural trait root morphological trait |
| title | High-Throughput Root Imaging Analysis Reveals Wide Variation in Root Morphology of Wild Adzuki bean (<i>Vigna angularis</i>) Accessions |
| title_full | High-Throughput Root Imaging Analysis Reveals Wide Variation in Root Morphology of Wild Adzuki bean (<i>Vigna angularis</i>) Accessions |
| title_fullStr | High-Throughput Root Imaging Analysis Reveals Wide Variation in Root Morphology of Wild Adzuki bean (<i>Vigna angularis</i>) Accessions |
| title_full_unstemmed | High-Throughput Root Imaging Analysis Reveals Wide Variation in Root Morphology of Wild Adzuki bean (<i>Vigna angularis</i>) Accessions |
| title_short | High-Throughput Root Imaging Analysis Reveals Wide Variation in Root Morphology of Wild Adzuki bean (<i>Vigna angularis</i>) Accessions |
| title_sort | high throughput root imaging analysis reveals wide variation in root morphology of wild adzuki bean i vigna angularis i accessions |
| topic | cluster analysis image analysis legume root architectural trait root morphological trait |
| url | https://www.mdpi.com/2223-7747/11/3/405 |
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