Genetic diversity of toxigenic Vibrio cholerae O1 from Sabah, Malaysia 2015
Background: Cholera is an important health problem in Sabah, a Malaysian state in northern Borneo; however, Vibrio cholerae in Sabah have never been characterized. Since 2002, serogroup O1 strains having the traits of both classical and El Tor biotype, designated as atypical El Tor biotype, have bee...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-08-01
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| Series: | Journal of Microbiology, Immunology and Infection |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1684118218300070 |
| _version_ | 1831660022014148608 |
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| author | Myo Thura Zaw Nor Amalina Emran Mohd Yusof Ibrahim Maria Suleiman Tajul Ariffin Awang Mohd Aza Sherin Yusuff Khin Saw Naing Than Myint Muhammad Jikal Mohd Azmi Salleh Zaw Lin |
| author_facet | Myo Thura Zaw Nor Amalina Emran Mohd Yusof Ibrahim Maria Suleiman Tajul Ariffin Awang Mohd Aza Sherin Yusuff Khin Saw Naing Than Myint Muhammad Jikal Mohd Azmi Salleh Zaw Lin |
| author_sort | Myo Thura Zaw |
| collection | DOAJ |
| description | Background: Cholera is an important health problem in Sabah, a Malaysian state in northern Borneo; however, Vibrio cholerae in Sabah have never been characterized. Since 2002, serogroup O1 strains having the traits of both classical and El Tor biotype, designated as atypical El Tor biotype, have been increasingly reported as the cause of cholera worldwide. These variants are believed to produce clinically more severe disease like classical strains. Purpose: The purpose of this study is to investigate the genetic diversity of V. cholerae in Sabah and whether V. cholerae in Sabah belong to atypical El Tor biotype. Methods: ERIC-PCR, a DNA fingerprinting method for bacterial pathogens based on the enterobacterial repetitive intergenic consensus sequence, was used to study the genetic diversity of 65 clinical V. cholerae O1 isolates from 3 districts (Kudat, Beluran, Sandakan) in Sabah and one environmental isolate from coastal sea water in Kudat district. In addition, we studied the biotype-specific genetic traits in these isolates to establish their biotype. Results: Different fingerprint patterns were seen in isolates from these three districts but one of the patterns was seen in more than one district. Clinical isolates and environmental isolate have different patterns. In addition, Sabah isolates harbor genetic traits specific to both classical biotype (ctxB-1, rstRCla) and El Tor biotype (rstRET, rstC, tcpAET, rtxC, VC2346). Conclusion: This study revealed that V. cholerae in Sabah were genetically diverse and were atypical El Tor strains. Fingerprint patterns of these isolates will be useful in tracing the origin of this pathogen in the future. Keywords: Cholera, Vibrio cholerae, Genetic diversity, Atypical El Tor, Sabah |
| first_indexed | 2024-12-19T18:08:40Z |
| format | Article |
| id | doaj.art-9332d127f994489fab28a993d78fc1ca |
| institution | Directory Open Access Journal |
| issn | 1684-1182 |
| language | English |
| last_indexed | 2024-12-19T18:08:40Z |
| publishDate | 2019-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Microbiology, Immunology and Infection |
| spelling | doaj.art-9332d127f994489fab28a993d78fc1ca2022-12-21T20:11:23ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822019-08-01524563570Genetic diversity of toxigenic Vibrio cholerae O1 from Sabah, Malaysia 2015Myo Thura Zaw0Nor Amalina Emran1Mohd Yusof Ibrahim2Maria Suleiman3Tajul Ariffin Awang Mohd4Aza Sherin Yusuff5Khin Saw Naing6Than Myint7Muhammad Jikal8Mohd Azmi Salleh9Zaw Lin10Pathobiological and Medical Diagnostics Department, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, MalaysiaPathobiological and Medical Diagnostics Department, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, MalaysiaDepartment of Community Medicine, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, MalaysiaSabah State Health Department, Jalan Tunku Abdul Rahman, Bandaran, 88000 Kota Kinabalu, Sabah, MalaysiaKota Kinabalu Public Health Laboratory, Bukit Padang, Jalan Kolam, 88850 Kota Kinabalu, Sabah, MalaysiaDepartment of Community Medicine, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, MalaysiaDepartment of Community Medicine, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, MalaysiaDepartment of Community Medicine, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, MalaysiaKudat Area Health Office, 89509 Kudat, Sabah, MalaysiaPathology Department, Hospital Duchess of Kent, KM3.2 Jalan Utara, 9000 Sandakan, Sabah, MalaysiaPathobiological and Medical Diagnostics Department, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia; Corresponding author.Background: Cholera is an important health problem in Sabah, a Malaysian state in northern Borneo; however, Vibrio cholerae in Sabah have never been characterized. Since 2002, serogroup O1 strains having the traits of both classical and El Tor biotype, designated as atypical El Tor biotype, have been increasingly reported as the cause of cholera worldwide. These variants are believed to produce clinically more severe disease like classical strains. Purpose: The purpose of this study is to investigate the genetic diversity of V. cholerae in Sabah and whether V. cholerae in Sabah belong to atypical El Tor biotype. Methods: ERIC-PCR, a DNA fingerprinting method for bacterial pathogens based on the enterobacterial repetitive intergenic consensus sequence, was used to study the genetic diversity of 65 clinical V. cholerae O1 isolates from 3 districts (Kudat, Beluran, Sandakan) in Sabah and one environmental isolate from coastal sea water in Kudat district. In addition, we studied the biotype-specific genetic traits in these isolates to establish their biotype. Results: Different fingerprint patterns were seen in isolates from these three districts but one of the patterns was seen in more than one district. Clinical isolates and environmental isolate have different patterns. In addition, Sabah isolates harbor genetic traits specific to both classical biotype (ctxB-1, rstRCla) and El Tor biotype (rstRET, rstC, tcpAET, rtxC, VC2346). Conclusion: This study revealed that V. cholerae in Sabah were genetically diverse and were atypical El Tor strains. Fingerprint patterns of these isolates will be useful in tracing the origin of this pathogen in the future. Keywords: Cholera, Vibrio cholerae, Genetic diversity, Atypical El Tor, Sabahhttp://www.sciencedirect.com/science/article/pii/S1684118218300070 |
| spellingShingle | Myo Thura Zaw Nor Amalina Emran Mohd Yusof Ibrahim Maria Suleiman Tajul Ariffin Awang Mohd Aza Sherin Yusuff Khin Saw Naing Than Myint Muhammad Jikal Mohd Azmi Salleh Zaw Lin Genetic diversity of toxigenic Vibrio cholerae O1 from Sabah, Malaysia 2015 Journal of Microbiology, Immunology and Infection |
| title | Genetic diversity of toxigenic Vibrio cholerae O1 from Sabah, Malaysia 2015 |
| title_full | Genetic diversity of toxigenic Vibrio cholerae O1 from Sabah, Malaysia 2015 |
| title_fullStr | Genetic diversity of toxigenic Vibrio cholerae O1 from Sabah, Malaysia 2015 |
| title_full_unstemmed | Genetic diversity of toxigenic Vibrio cholerae O1 from Sabah, Malaysia 2015 |
| title_short | Genetic diversity of toxigenic Vibrio cholerae O1 from Sabah, Malaysia 2015 |
| title_sort | genetic diversity of toxigenic vibrio cholerae o1 from sabah malaysia 2015 |
| url | http://www.sciencedirect.com/science/article/pii/S1684118218300070 |
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