Integrative Analysis of Human Macrophage Inflammatory Response Related to Mycobacterium tuberculosis Virulence
Mycobacterium tuberculosis (Mtb), the etiological agent of tuberculosis, kills 1.5 to 1.7 million people every year. Macrophages are Mtb’s main host cells and their inflammatory response is an essential component of the host defense against Mtb. However, Mtb is able to circumvent the macrophages’ de...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.668060/full |
| _version_ | 1818662810266107904 |
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| author | Pauline Bade Pauline Bade Fabrizio Simonetti Stephanie Sans Patricia Laboudie Khadija Kissane Nicolas Chappat Sophie Lagrange Florence Apparailly Christine Roubert Isabelle Duroux-Richard |
| author_facet | Pauline Bade Pauline Bade Fabrizio Simonetti Stephanie Sans Patricia Laboudie Khadija Kissane Nicolas Chappat Sophie Lagrange Florence Apparailly Christine Roubert Isabelle Duroux-Richard |
| author_sort | Pauline Bade |
| collection | DOAJ |
| description | Mycobacterium tuberculosis (Mtb), the etiological agent of tuberculosis, kills 1.5 to 1.7 million people every year. Macrophages are Mtb’s main host cells and their inflammatory response is an essential component of the host defense against Mtb. However, Mtb is able to circumvent the macrophages’ defenses by triggering an inappropriate inflammatory response. The ability of Mtb to hinder phagolysosome maturation and acidification, and to escape the phagosome into the cytosol, is closely linked to its virulence. The modulation of the host inflammatory response relies on Mtb virulence factors, but remains poorly studied. Understanding macrophage interactions with Mtb is crucial to develop strategies to control tuberculosis. The present study aims to determine the inflammatory response transcriptome and miRNome of human macrophages infected with the virulent H37Rv Mtb strain, to identify macrophage genetic networks specifically modulated by Mtb virulence. Using human macrophages infected with two different live strains of mycobacteria (live or heat-inactivated Mtb H37Rv and M. marinum), we quantified and analyzed 184 inflammatory mRNAs and 765 micro(mi)RNAs. Transcripts and miRNAs differently modulated by H37Rv in comparison with the two other conditions were analyzed using in silico approaches. We identified 30 host inflammatory response genes and 37 miRNAs specific for H37Rv virulence, and highlight evidence suggesting that Mtb intracellular-linked virulence depends on the inhibition of IL-1β-dependent pro-inflammatory response, the repression of apoptosis and the delay of the recruitment and activation of adaptive immune cells. Our findings provide new potential targets for the development of macrophage-based therapeutic strategies against TB. |
| first_indexed | 2024-12-17T05:06:52Z |
| format | Article |
| id | doaj.art-933aae839b0640d6aaca6845fbf0a6b1 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-17T05:06:52Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-933aae839b0640d6aaca6845fbf0a6b12022-12-21T22:02:23ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-06-011210.3389/fimmu.2021.668060668060Integrative Analysis of Human Macrophage Inflammatory Response Related to Mycobacterium tuberculosis VirulencePauline Bade0Pauline Bade1Fabrizio Simonetti2Stephanie Sans3Patricia Laboudie4Khadija Kissane5Nicolas Chappat6Sophie Lagrange7Florence Apparailly8Christine Roubert9Isabelle Duroux-Richard10Institute for Regenerative Medicine & Biotherapy (IRMB), INSERM, Univ Montpellier, CHU Montpellier, Montpellier, FranceEvotec ID (Lyon), Lyon, FranceEvotec ID (Lyon), Lyon, FranceEvotec ID (Lyon), Lyon, FranceEvotec ID (Lyon), Lyon, FranceEvotec ID (Lyon), Lyon, FranceEvotec ID (Lyon), Lyon, FranceEvotec ID (Lyon), Lyon, FranceInstitute for Regenerative Medicine & Biotherapy (IRMB), INSERM, Univ Montpellier, CHU Montpellier, Montpellier, FranceEvotec ID (Lyon), Lyon, FranceInstitute for Regenerative Medicine & Biotherapy (IRMB), INSERM, Univ Montpellier, CHU Montpellier, Montpellier, FranceMycobacterium tuberculosis (Mtb), the etiological agent of tuberculosis, kills 1.5 to 1.7 million people every year. Macrophages are Mtb’s main host cells and their inflammatory response is an essential component of the host defense against Mtb. However, Mtb is able to circumvent the macrophages’ defenses by triggering an inappropriate inflammatory response. The ability of Mtb to hinder phagolysosome maturation and acidification, and to escape the phagosome into the cytosol, is closely linked to its virulence. The modulation of the host inflammatory response relies on Mtb virulence factors, but remains poorly studied. Understanding macrophage interactions with Mtb is crucial to develop strategies to control tuberculosis. The present study aims to determine the inflammatory response transcriptome and miRNome of human macrophages infected with the virulent H37Rv Mtb strain, to identify macrophage genetic networks specifically modulated by Mtb virulence. Using human macrophages infected with two different live strains of mycobacteria (live or heat-inactivated Mtb H37Rv and M. marinum), we quantified and analyzed 184 inflammatory mRNAs and 765 micro(mi)RNAs. Transcripts and miRNAs differently modulated by H37Rv in comparison with the two other conditions were analyzed using in silico approaches. We identified 30 host inflammatory response genes and 37 miRNAs specific for H37Rv virulence, and highlight evidence suggesting that Mtb intracellular-linked virulence depends on the inhibition of IL-1β-dependent pro-inflammatory response, the repression of apoptosis and the delay of the recruitment and activation of adaptive immune cells. Our findings provide new potential targets for the development of macrophage-based therapeutic strategies against TB.https://www.frontiersin.org/articles/10.3389/fimmu.2021.668060/fullmiRNAMycobacterium tuberculosisMycobacterium marinummacrophagehost responseinflammatory response |
| spellingShingle | Pauline Bade Pauline Bade Fabrizio Simonetti Stephanie Sans Patricia Laboudie Khadija Kissane Nicolas Chappat Sophie Lagrange Florence Apparailly Christine Roubert Isabelle Duroux-Richard Integrative Analysis of Human Macrophage Inflammatory Response Related to Mycobacterium tuberculosis Virulence Frontiers in Immunology miRNA Mycobacterium tuberculosis Mycobacterium marinum macrophage host response inflammatory response |
| title | Integrative Analysis of Human Macrophage Inflammatory Response Related to Mycobacterium tuberculosis Virulence |
| title_full | Integrative Analysis of Human Macrophage Inflammatory Response Related to Mycobacterium tuberculosis Virulence |
| title_fullStr | Integrative Analysis of Human Macrophage Inflammatory Response Related to Mycobacterium tuberculosis Virulence |
| title_full_unstemmed | Integrative Analysis of Human Macrophage Inflammatory Response Related to Mycobacterium tuberculosis Virulence |
| title_short | Integrative Analysis of Human Macrophage Inflammatory Response Related to Mycobacterium tuberculosis Virulence |
| title_sort | integrative analysis of human macrophage inflammatory response related to mycobacterium tuberculosis virulence |
| topic | miRNA Mycobacterium tuberculosis Mycobacterium marinum macrophage host response inflammatory response |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.668060/full |
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