Prevalence and antibiogram of Extended Spectrum β-Lactamase (ESBL) producing Gram negative bacilli and further molecular characterization of ESBL producing Escherichia coli and Klebsiella spp.
Background and Objectives: Resistance to a wide variety of common antimicrobials has made the proliferation of Extended spectrum β-lactmase (ESBL) producing strains a serious global health concern that has complicated treatment strategies. The high proportion of ESBL producers among the Enterob...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2013-10-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/3462/19-%206460_E(RT)_F(H)_PF1(PUH)_PFA(H)_OLF_PFA(H)_PFA(H)_PF2(Bo_PUH).pdf |
| Summary: | Background and Objectives: Resistance to a wide variety
of common antimicrobials has made the proliferation of
Extended spectrum β-lactmase (ESBL) producing strains a
serious global health concern that has complicated treatment
strategies. The high proportion of ESBL producers among the
Enterobacteriaceae and the complex molecular epidemiology
with diverse types of ESBL genes are alarming. This study
was undertaken to identify ESBL production in various Gram
negative bacilli isolated and to further characterize ESBL
producers among Escherichia coli and Klebsiella spp by PCR,
which were initially screened by phenotypic method.
Materials and Methods: A total of 722 isolates of Gram
negative bacilli were isolated. Presence of ESBL positivity
was detected using the double disk synergy test (DDST). Their
antibiogram was studied. PCR analysis for β-lactamase (bla)
genes of the family TEM, SHV and CTX-M was also carried out
using designed primers in 20 ESBL isolates each of Escherichia
coli and Klebsiella spp.
Results: Among 722 Gram negative bacilli isolated 379 (52.49%)
were ESBL producers. The major source of ESBL producers
were respiratory tract samples, highest ESBL production was
observed in Klebsiella sp. (67.04%). Resistance to multiple
classes of antibiotics was observed among ESBL producers.
Among ESBL producing genes prevalence of bla-CTX-M (82.5%)
was highest, followed by bla-TEM (67.5%) and bla-SHV (57.5%)
in the present study. The frequency of ESBL producing strains
among clinical isolates has been steadily increasing. Advance
drug resistance surveillance and molecular characteristics
of ESBL isolates is necessary to guide the appropriate and
judicious antibiotic use. |
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| ISSN: | 2249-782X 0973-709X |