Seed Priming with Brassinosteroids Alleviates Chromium Stress in Rice Cultivars via Improving ROS Metabolism and Antioxidant Defense Response at Biochemical and Molecular Levels
This research was performed to explore the vital role of seed priming with a 0.01 µM concentration of brassinosteroids (EBL) to alleviate the adverse effects of Cr (100 µM) in two different rice cultivars. Seed priming with EBL significantly enhanced the germination attributes (germination percentag...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/7/1089 |
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| author | Farwa Basit Min Chen Temoor Ahmed Muhammad Shahid Muhammad Noman Jiaxin Liu Jianyu An Abeer Hashem Al-Bandari Fahad Al-Arjani Abdulaziz A. Alqarawi Mashail Fahad S. Alsayed Elsayed Fathi Abd_Allah Jin Hu Yajing Guan |
| author_facet | Farwa Basit Min Chen Temoor Ahmed Muhammad Shahid Muhammad Noman Jiaxin Liu Jianyu An Abeer Hashem Al-Bandari Fahad Al-Arjani Abdulaziz A. Alqarawi Mashail Fahad S. Alsayed Elsayed Fathi Abd_Allah Jin Hu Yajing Guan |
| author_sort | Farwa Basit |
| collection | DOAJ |
| description | This research was performed to explore the vital role of seed priming with a 0.01 µM concentration of brassinosteroids (EBL) to alleviate the adverse effects of Cr (100 µM) in two different rice cultivars. Seed priming with EBL significantly enhanced the germination attributes (germination percentage, germination energy, germination index, and vigor index, etc.), photosynthetic rate as well as plant growth (shoot and root length including the fresh and dry weight) under Cr toxicity as compared to the plants primed with water. Cr toxicity induced antioxidant enzyme activities (SOD, POD, CAT, and APX) and ROS level (MDA and H<sub>2</sub>O<sub>2</sub> contents) in both rice cultivars; however, a larger increment was observed in YLY-689 (tolerant) than CY-927 (sensitive) cultivar. EBL application stimulatingly increased antioxidant enzyme activities to scavenge ROS production under Cr stress. The gene expression of SOD and POD in EBL-primed rice plants followed a similar increasing trend as observed in the case of enzymatic activities of SOD and POD compared to water-primed rice plants. Simultaneously, Cr uptake was observed to be significantly higher in the water-primed control compared to plants primed with EBL. Moreover, Cr uptake was significant in YLY-689 compared to CY-927. In ultra-structure studies, it was observed that EBL priming relieved the rice plants from sub-cellular damage. Conclusively, our research indicated that seed priming with EBL could be adopted as a promising strategy to enhance rice growth by copping the venomous effect of Cr. |
| first_indexed | 2024-03-10T09:47:05Z |
| format | Article |
| id | doaj.art-933bb0e28f2c42b3ac91b8b953ada4ae |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T09:47:05Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-933bb0e28f2c42b3ac91b8b953ada4ae2023-11-22T03:05:16ZengMDPI AGAntioxidants2076-39212021-07-01107108910.3390/antiox10071089Seed Priming with Brassinosteroids Alleviates Chromium Stress in Rice Cultivars via Improving ROS Metabolism and Antioxidant Defense Response at Biochemical and Molecular LevelsFarwa Basit0Min Chen1Temoor Ahmed2Muhammad Shahid3Muhammad Noman4Jiaxin Liu5Jianyu An6Abeer Hashem7Al-Bandari Fahad Al-Arjani8Abdulaziz A. Alqarawi9Mashail Fahad S. Alsayed10Elsayed Fathi Abd_Allah11Jin Hu12Yajing Guan13Institute of Crop Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaInstitute of Crop Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaDepartment of Bioinformatics and Biotechnology, Government College University, Faisalabad 38000, PakistanState Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaInstitute of Crop Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaInstitute of Crop Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaBotany and Microbiology Department, College of Science, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaBotany and Microbiology Department, College of Science, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaPlant Production Department, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaBotany and Microbiology Department, College of Science, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaPlant Production Department, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaInstitute of Crop Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaInstitute of Crop Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaThis research was performed to explore the vital role of seed priming with a 0.01 µM concentration of brassinosteroids (EBL) to alleviate the adverse effects of Cr (100 µM) in two different rice cultivars. Seed priming with EBL significantly enhanced the germination attributes (germination percentage, germination energy, germination index, and vigor index, etc.), photosynthetic rate as well as plant growth (shoot and root length including the fresh and dry weight) under Cr toxicity as compared to the plants primed with water. Cr toxicity induced antioxidant enzyme activities (SOD, POD, CAT, and APX) and ROS level (MDA and H<sub>2</sub>O<sub>2</sub> contents) in both rice cultivars; however, a larger increment was observed in YLY-689 (tolerant) than CY-927 (sensitive) cultivar. EBL application stimulatingly increased antioxidant enzyme activities to scavenge ROS production under Cr stress. The gene expression of SOD and POD in EBL-primed rice plants followed a similar increasing trend as observed in the case of enzymatic activities of SOD and POD compared to water-primed rice plants. Simultaneously, Cr uptake was observed to be significantly higher in the water-primed control compared to plants primed with EBL. Moreover, Cr uptake was significant in YLY-689 compared to CY-927. In ultra-structure studies, it was observed that EBL priming relieved the rice plants from sub-cellular damage. Conclusively, our research indicated that seed priming with EBL could be adopted as a promising strategy to enhance rice growth by copping the venomous effect of Cr.https://www.mdpi.com/2076-3921/10/7/1089antioxidantsbrassinosteroidschromiumheavy metalsrice |
| spellingShingle | Farwa Basit Min Chen Temoor Ahmed Muhammad Shahid Muhammad Noman Jiaxin Liu Jianyu An Abeer Hashem Al-Bandari Fahad Al-Arjani Abdulaziz A. Alqarawi Mashail Fahad S. Alsayed Elsayed Fathi Abd_Allah Jin Hu Yajing Guan Seed Priming with Brassinosteroids Alleviates Chromium Stress in Rice Cultivars via Improving ROS Metabolism and Antioxidant Defense Response at Biochemical and Molecular Levels Antioxidants antioxidants brassinosteroids chromium heavy metals rice |
| title | Seed Priming with Brassinosteroids Alleviates Chromium Stress in Rice Cultivars via Improving ROS Metabolism and Antioxidant Defense Response at Biochemical and Molecular Levels |
| title_full | Seed Priming with Brassinosteroids Alleviates Chromium Stress in Rice Cultivars via Improving ROS Metabolism and Antioxidant Defense Response at Biochemical and Molecular Levels |
| title_fullStr | Seed Priming with Brassinosteroids Alleviates Chromium Stress in Rice Cultivars via Improving ROS Metabolism and Antioxidant Defense Response at Biochemical and Molecular Levels |
| title_full_unstemmed | Seed Priming with Brassinosteroids Alleviates Chromium Stress in Rice Cultivars via Improving ROS Metabolism and Antioxidant Defense Response at Biochemical and Molecular Levels |
| title_short | Seed Priming with Brassinosteroids Alleviates Chromium Stress in Rice Cultivars via Improving ROS Metabolism and Antioxidant Defense Response at Biochemical and Molecular Levels |
| title_sort | seed priming with brassinosteroids alleviates chromium stress in rice cultivars via improving ros metabolism and antioxidant defense response at biochemical and molecular levels |
| topic | antioxidants brassinosteroids chromium heavy metals rice |
| url | https://www.mdpi.com/2076-3921/10/7/1089 |
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