Pollen–pistil interaction in self-pollinated and sib pollinated flowers of sunnhemp (Crotalaria juncea L.)
Pollen pistil interaction studies were carried out in six sunnhemp genotypes. Pollen viability percentage of the six genotypes ranged from 0.0 to 93.5% and pollen germination ranged from 0.0 to 84.0% at four different floral stages starting from bud to opening of a flower. The viable pollen grains...
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| Format: | Article |
| Language: | English |
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Indian Society of Plant Breeders
2022-06-01
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| Series: | Electronic Journal of Plant Breeding |
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| Online Access: | https://www.ejplantbreeding.org/index.php/EJPB/article/view/4317 |
| _version_ | 1827973335197155328 |
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| author | P. Suriya1, N. Meenakshi ganesan1*, A. Subramanian1 and R. Gnanam2 |
| author_facet | P. Suriya1, N. Meenakshi ganesan1*, A. Subramanian1 and R. Gnanam2 |
| author_sort | P. Suriya1, N. Meenakshi ganesan1*, A. Subramanian1 and R. Gnanam2 |
| collection | DOAJ |
| description | Pollen pistil interaction studies were carried out in six sunnhemp genotypes. Pollen viability percentage of the six
genotypes ranged from 0.0 to 93.5% and pollen germination ranged from 0.0 to 84.0% at four different floral stages
starting from bud to opening of a flower. The viable pollen grains were present at the later three stages of flower for
globose type anthers, while the pollen grains were viable at the later two floral stages for heart shaped anthers. Stigma
receptivity was high to very high during S3 and S4 stages. Artificial self-pollination and sib pollination with a mixture
of pollen grains were carried out and in vivo pollen tube growth in the style was investigated using fluorescence
microscopy. In self-pollinated pistils, eight hours after pollination, callose wall development was seen around the
nucellus cell. However, under sib pollinated pistil, eight hours after pollination, pollen tube penetrated into embryo
sac of an ovule. The formation of callose in self-fertilized pistil prevented fertilization and seed set. But sib pollinated
flowers were fertilized normally and a seed set could be observed. The investigation proved the existence of selfincompatibility. |
| first_indexed | 2024-04-09T19:34:42Z |
| format | Article |
| id | doaj.art-93424f1b7fd143ae93526508bf6fcbbf |
| institution | Directory Open Access Journal |
| issn | 0975-928X |
| language | English |
| last_indexed | 2024-04-09T19:34:42Z |
| publishDate | 2022-06-01 |
| publisher | Indian Society of Plant Breeders |
| record_format | Article |
| series | Electronic Journal of Plant Breeding |
| spelling | doaj.art-93424f1b7fd143ae93526508bf6fcbbf2023-04-04T16:11:10ZengIndian Society of Plant BreedersElectronic Journal of Plant Breeding0975-928X2022-06-0113228629410.37992/2022.1302.061Pollen–pistil interaction in self-pollinated and sib pollinated flowers of sunnhemp (Crotalaria juncea L.)P. Suriya1, N. Meenakshi ganesan1*, A. Subramanian1 and R. Gnanam201Department of Genetics and Plant Breeding, TNAU, Coimbatore, Tamil Nadu, India. 2Department of Plant Molecular Biology and Bioinformatics, TNAU, Coimbatore, Tamil Nadu, India. *E-Mail : suriya100898@gmail.comPollen pistil interaction studies were carried out in six sunnhemp genotypes. Pollen viability percentage of the six genotypes ranged from 0.0 to 93.5% and pollen germination ranged from 0.0 to 84.0% at four different floral stages starting from bud to opening of a flower. The viable pollen grains were present at the later three stages of flower for globose type anthers, while the pollen grains were viable at the later two floral stages for heart shaped anthers. Stigma receptivity was high to very high during S3 and S4 stages. Artificial self-pollination and sib pollination with a mixture of pollen grains were carried out and in vivo pollen tube growth in the style was investigated using fluorescence microscopy. In self-pollinated pistils, eight hours after pollination, callose wall development was seen around the nucellus cell. However, under sib pollinated pistil, eight hours after pollination, pollen tube penetrated into embryo sac of an ovule. The formation of callose in self-fertilized pistil prevented fertilization and seed set. But sib pollinated flowers were fertilized normally and a seed set could be observed. The investigation proved the existence of selfincompatibility.https://www.ejplantbreeding.org/index.php/EJPB/article/view/4317sunnhempcrotalariapollinationself-incompatibilitycallose |
| spellingShingle | P. Suriya1, N. Meenakshi ganesan1*, A. Subramanian1 and R. Gnanam2 Pollen–pistil interaction in self-pollinated and sib pollinated flowers of sunnhemp (Crotalaria juncea L.) Electronic Journal of Plant Breeding sunnhemp crotalaria pollination self-incompatibility callose |
| title | Pollen–pistil interaction in self-pollinated and sib pollinated flowers of sunnhemp (Crotalaria juncea L.) |
| title_full | Pollen–pistil interaction in self-pollinated and sib pollinated flowers of sunnhemp (Crotalaria juncea L.) |
| title_fullStr | Pollen–pistil interaction in self-pollinated and sib pollinated flowers of sunnhemp (Crotalaria juncea L.) |
| title_full_unstemmed | Pollen–pistil interaction in self-pollinated and sib pollinated flowers of sunnhemp (Crotalaria juncea L.) |
| title_short | Pollen–pistil interaction in self-pollinated and sib pollinated flowers of sunnhemp (Crotalaria juncea L.) |
| title_sort | pollen pistil interaction in self pollinated and sib pollinated flowers of sunnhemp crotalaria juncea l |
| topic | sunnhemp crotalaria pollination self-incompatibility callose |
| url | https://www.ejplantbreeding.org/index.php/EJPB/article/view/4317 |
| work_keys_str_mv | AT psuriya1nmeenakshiganesan1asubramanian1andrgnanam2 pollenpistilinteractioninselfpollinatedandsibpollinatedflowersofsunnhempcrotalariajunceal |