Cell-type heterogeneity in the early zebrafish olfactory epithelium is generated from progenitors within preplacodal ectoderm
The zebrafish olfactory epithelium comprises a variety of neuronal populations, which are thought to have distinct embryonic origins. For instance, while ciliated sensory neurons arise from preplacodal ectoderm (PPE), previous lineage tracing studies suggest that both Gonadotropin releasing hormone...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-01-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/32041 |
| _version_ | 1818019838531993600 |
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| author | Raphaël Aguillon Julie Batut Arul Subramanian Romain Madelaine Pascale Dufourcq Thomas F Schilling Patrick Blader |
| author_facet | Raphaël Aguillon Julie Batut Arul Subramanian Romain Madelaine Pascale Dufourcq Thomas F Schilling Patrick Blader |
| author_sort | Raphaël Aguillon |
| collection | DOAJ |
| description | The zebrafish olfactory epithelium comprises a variety of neuronal populations, which are thought to have distinct embryonic origins. For instance, while ciliated sensory neurons arise from preplacodal ectoderm (PPE), previous lineage tracing studies suggest that both Gonadotropin releasing hormone 3 (Gnrh3) and microvillous sensory neurons derive from cranial neural crest (CNC). We find that the expression of Islet1/2 is restricted to Gnrh3 neurons associated with the olfactory epithelium. Unexpectedly, however, we find no change in Islet1/2+ cell numbers in sox10 mutant embryos, calling into question their CNC origin. Lineage reconstruction based on backtracking in time-lapse confocal datasets, and confirmed by photoconversion experiments, reveals that Gnrh3 neurons derive from the anterior PPE. Similarly, all of the microvillous sensory neurons we have traced arise from preplacodal progenitors. Our results suggest that rather than originating from separate ectodermal populations, cell-type heterogeneity is generated from overlapping pools of progenitors within the preplacodal ectoderm. |
| first_indexed | 2024-04-14T07:57:43Z |
| format | Article |
| id | doaj.art-9342fd89a923408f816c8c7ed53c6335 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:57:43Z |
| publishDate | 2018-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-9342fd89a923408f816c8c7ed53c63352022-12-22T02:05:00ZengeLife Sciences Publications LtdeLife2050-084X2018-01-01710.7554/eLife.32041Cell-type heterogeneity in the early zebrafish olfactory epithelium is generated from progenitors within preplacodal ectodermRaphaël Aguillon0Julie Batut1https://orcid.org/0000-0002-1984-2094Arul Subramanian2https://orcid.org/0000-0001-8455-6804Romain Madelaine3Pascale Dufourcq4Thomas F Schilling5https://orcid.org/0000-0003-1798-8695Patrick Blader6https://orcid.org/0000-0003-3299-6108Centre de Biologie du Développement (CBD, UMR5547), Centre de Biologie Intégrative (CBI, FR3743), Université de Toulouse, Toulouse, FranceCentre de Biologie du Développement (CBD, UMR5547), Centre de Biologie Intégrative (CBI, FR3743), Université de Toulouse, Toulouse, FranceDepartment of Developmental and Cell Biology, University of California, Irvine, United StatesCentre de Biologie du Développement (CBD, UMR5547), Centre de Biologie Intégrative (CBI, FR3743), Université de Toulouse, Toulouse, FranceCentre de Biologie du Développement (CBD, UMR5547), Centre de Biologie Intégrative (CBI, FR3743), Université de Toulouse, Toulouse, FranceDepartment of Developmental and Cell Biology, University of California, Irvine, United StatesCentre de Biologie du Développement (CBD, UMR5547), Centre de Biologie Intégrative (CBI, FR3743), Université de Toulouse, Toulouse, FranceThe zebrafish olfactory epithelium comprises a variety of neuronal populations, which are thought to have distinct embryonic origins. For instance, while ciliated sensory neurons arise from preplacodal ectoderm (PPE), previous lineage tracing studies suggest that both Gonadotropin releasing hormone 3 (Gnrh3) and microvillous sensory neurons derive from cranial neural crest (CNC). We find that the expression of Islet1/2 is restricted to Gnrh3 neurons associated with the olfactory epithelium. Unexpectedly, however, we find no change in Islet1/2+ cell numbers in sox10 mutant embryos, calling into question their CNC origin. Lineage reconstruction based on backtracking in time-lapse confocal datasets, and confirmed by photoconversion experiments, reveals that Gnrh3 neurons derive from the anterior PPE. Similarly, all of the microvillous sensory neurons we have traced arise from preplacodal progenitors. Our results suggest that rather than originating from separate ectodermal populations, cell-type heterogeneity is generated from overlapping pools of progenitors within the preplacodal ectoderm.https://elifesciences.org/articles/32041olfactory placodeneuronal subtypelineage origin |
| spellingShingle | Raphaël Aguillon Julie Batut Arul Subramanian Romain Madelaine Pascale Dufourcq Thomas F Schilling Patrick Blader Cell-type heterogeneity in the early zebrafish olfactory epithelium is generated from progenitors within preplacodal ectoderm eLife olfactory placode neuronal subtype lineage origin |
| title | Cell-type heterogeneity in the early zebrafish olfactory epithelium is generated from progenitors within preplacodal ectoderm |
| title_full | Cell-type heterogeneity in the early zebrafish olfactory epithelium is generated from progenitors within preplacodal ectoderm |
| title_fullStr | Cell-type heterogeneity in the early zebrafish olfactory epithelium is generated from progenitors within preplacodal ectoderm |
| title_full_unstemmed | Cell-type heterogeneity in the early zebrafish olfactory epithelium is generated from progenitors within preplacodal ectoderm |
| title_short | Cell-type heterogeneity in the early zebrafish olfactory epithelium is generated from progenitors within preplacodal ectoderm |
| title_sort | cell type heterogeneity in the early zebrafish olfactory epithelium is generated from progenitors within preplacodal ectoderm |
| topic | olfactory placode neuronal subtype lineage origin |
| url | https://elifesciences.org/articles/32041 |
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