Identification and Characterization of <i>Hdh-FMRF2</i> Gene in Pacific Abalone and Its Possible Role in Reproduction and Larva Development
FMRFamide-related peptides are neuropeptides involved in a wide range of biological processes, including reproduction and larval development. To characterize the involvement of FMRFamide in the reproduction and larval development of Pacific abalone <i>Haliotis discus hannai</i>, an FMRFa...
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MDPI AG
2023-01-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/1/109 |
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| author | Zahid Parvez Sukhan Yusin Cho Shaharior Hossen Won Kyo Lee Kang Hee Kho |
| author_facet | Zahid Parvez Sukhan Yusin Cho Shaharior Hossen Won Kyo Lee Kang Hee Kho |
| author_sort | Zahid Parvez Sukhan |
| collection | DOAJ |
| description | FMRFamide-related peptides are neuropeptides involved in a wide range of biological processes, including reproduction and larval development. To characterize the involvement of FMRFamide in the reproduction and larval development of Pacific abalone <i>Haliotis discus hannai</i>, an FMRFamide cDNA (<i>Hdh-FMRF2</i>) was cloned from the cerebral ganglion (CG). Fluorescence in situ hybridization and qRT-PCR were performed for functional characterization. The <i>Hdh-FMRF2</i> cDNA encoded 204 deduced amino acids that contained a putative signal peptide and four FaRP domains. The major population of <i>Hdh-FMRF2</i> neuronal cell bodies was localized in the cortex of CG. <i>Hdh-FMRF2</i> mRNA expression was significantly upregulated in CG during the mature stage of gonadal development and effective accumulative temperature (EAT) exposed abalone in both sexes. In the induced spawning event, <i>Hdh-FMRF2</i> expression was significantly upregulated during spawning in males. However, no upregulation was observed in females, suggesting <i>Hdh-FMRF2</i> might inhibit gamete release in female abalone. These results revealed <i>Hdh-FMRF2</i> as a reproduction related peptide. Furthermore, mRNA expression in larval development suggested that this peptide was also involved in larval development during development of Pacific abalone. Collectively, this study provides evidence of possible involvement of an FMRFamide neuropeptide in the reproduction and larval development of Pacific abalone. |
| first_indexed | 2024-03-09T13:27:25Z |
| format | Article |
| id | doaj.art-9343adb42cac4d9eb69e4346ad85b71f |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T13:27:25Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-9343adb42cac4d9eb69e4346ad85b71f2023-11-30T21:22:54ZengMDPI AGBiomolecules2218-273X2023-01-0113110910.3390/biom13010109Identification and Characterization of <i>Hdh-FMRF2</i> Gene in Pacific Abalone and Its Possible Role in Reproduction and Larva DevelopmentZahid Parvez Sukhan0Yusin Cho1Shaharior Hossen2Won Kyo Lee3Kang Hee Kho4Department of Fisheries Science, Chonnam National University, Yeosu 59626, Republic of KoreaDepartment of Fisheries Science, Chonnam National University, Yeosu 59626, Republic of KoreaDepartment of Fisheries Science, Chonnam National University, Yeosu 59626, Republic of KoreaDepartment of Fisheries Science, Chonnam National University, Yeosu 59626, Republic of KoreaDepartment of Fisheries Science, Chonnam National University, Yeosu 59626, Republic of KoreaFMRFamide-related peptides are neuropeptides involved in a wide range of biological processes, including reproduction and larval development. To characterize the involvement of FMRFamide in the reproduction and larval development of Pacific abalone <i>Haliotis discus hannai</i>, an FMRFamide cDNA (<i>Hdh-FMRF2</i>) was cloned from the cerebral ganglion (CG). Fluorescence in situ hybridization and qRT-PCR were performed for functional characterization. The <i>Hdh-FMRF2</i> cDNA encoded 204 deduced amino acids that contained a putative signal peptide and four FaRP domains. The major population of <i>Hdh-FMRF2</i> neuronal cell bodies was localized in the cortex of CG. <i>Hdh-FMRF2</i> mRNA expression was significantly upregulated in CG during the mature stage of gonadal development and effective accumulative temperature (EAT) exposed abalone in both sexes. In the induced spawning event, <i>Hdh-FMRF2</i> expression was significantly upregulated during spawning in males. However, no upregulation was observed in females, suggesting <i>Hdh-FMRF2</i> might inhibit gamete release in female abalone. These results revealed <i>Hdh-FMRF2</i> as a reproduction related peptide. Furthermore, mRNA expression in larval development suggested that this peptide was also involved in larval development during development of Pacific abalone. Collectively, this study provides evidence of possible involvement of an FMRFamide neuropeptide in the reproduction and larval development of Pacific abalone.https://www.mdpi.com/2218-273X/13/1/109FMRFamideFMRF2neuropeptidereproductionlarval developmentPacific abalone |
| spellingShingle | Zahid Parvez Sukhan Yusin Cho Shaharior Hossen Won Kyo Lee Kang Hee Kho Identification and Characterization of <i>Hdh-FMRF2</i> Gene in Pacific Abalone and Its Possible Role in Reproduction and Larva Development Biomolecules FMRFamide FMRF2 neuropeptide reproduction larval development Pacific abalone |
| title | Identification and Characterization of <i>Hdh-FMRF2</i> Gene in Pacific Abalone and Its Possible Role in Reproduction and Larva Development |
| title_full | Identification and Characterization of <i>Hdh-FMRF2</i> Gene in Pacific Abalone and Its Possible Role in Reproduction and Larva Development |
| title_fullStr | Identification and Characterization of <i>Hdh-FMRF2</i> Gene in Pacific Abalone and Its Possible Role in Reproduction and Larva Development |
| title_full_unstemmed | Identification and Characterization of <i>Hdh-FMRF2</i> Gene in Pacific Abalone and Its Possible Role in Reproduction and Larva Development |
| title_short | Identification and Characterization of <i>Hdh-FMRF2</i> Gene in Pacific Abalone and Its Possible Role in Reproduction and Larva Development |
| title_sort | identification and characterization of i hdh fmrf2 i gene in pacific abalone and its possible role in reproduction and larva development |
| topic | FMRFamide FMRF2 neuropeptide reproduction larval development Pacific abalone |
| url | https://www.mdpi.com/2218-273X/13/1/109 |
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