Generation of two iPSC lines (IMGTi001-A and IMGTi001-B) from human skin fibroblasts with ring chromosome 22
Skin fibroblasts from a patient with intellectual disability and ring chromosome 22 were reprogrammed into induced pluripotent stem cells (iPSCs) to establish a clonal stem cell lines, IMGTi001-A (iTAF5-29) and IMGTi001-B (iTAF5-32). Because of ring chromosome mitotic instability these cell lines sh...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-08-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506118302046 |
| _version_ | 1819195281244160000 |
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| author | T.V. Nikitina A.G. Menzorov A.A. Kashevarova M.M. Gridina A.A. Khabarova Yu.S. Yakovleva M.E. Lopatkina E.A. Kizilova S.A. Vasilyev O.L. Serov I.N. Lebedev |
| author_facet | T.V. Nikitina A.G. Menzorov A.A. Kashevarova M.M. Gridina A.A. Khabarova Yu.S. Yakovleva M.E. Lopatkina E.A. Kizilova S.A. Vasilyev O.L. Serov I.N. Lebedev |
| author_sort | T.V. Nikitina |
| collection | DOAJ |
| description | Skin fibroblasts from a patient with intellectual disability and ring chromosome 22 were reprogrammed into induced pluripotent stem cells (iPSCs) to establish a clonal stem cell lines, IMGTi001-A (iTAF5-29) and IMGTi001-B (iTAF5-32). Because of ring chromosome mitotic instability these cell lines show mosaic karyotypes with 46,XX,r(22) in >83% cells, 45,XX,-22 as minor class and sporadically cells with other karyotypes. Differentiation in derivatives of all three germ layers was shown in teratoma assay for IMGTi001-A, and in embryoid bodies for both cell lines. To our knowledge, human iPSC lines with ring chromosome are described for the first time. |
| first_indexed | 2024-12-23T02:10:16Z |
| format | Article |
| id | doaj.art-9344b4359702432ca779b7e21ed80c73 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-23T02:10:16Z |
| publishDate | 2018-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-9344b4359702432ca779b7e21ed80c732022-12-21T18:03:48ZengElsevierStem Cell Research1873-50612018-08-0131244248Generation of two iPSC lines (IMGTi001-A and IMGTi001-B) from human skin fibroblasts with ring chromosome 22T.V. Nikitina0A.G. Menzorov1A.A. Kashevarova2M.M. Gridina3A.A. Khabarova4Yu.S. Yakovleva5M.E. Lopatkina6E.A. Kizilova7S.A. Vasilyev8O.L. Serov9I.N. Lebedev10Research Institute of Medical Genetics, Tomsk NRMC, Tomsk, Russia; Corresponding author.Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, RussiaResearch Institute of Medical Genetics, Tomsk NRMC, Tomsk, RussiaInstitute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaResearch Institute of Medical Genetics, Tomsk NRMC, Tomsk, RussiaResearch Institute of Medical Genetics, Tomsk NRMC, Tomsk, RussiaInstitute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaResearch Institute of Medical Genetics, Tomsk NRMC, Tomsk, RussiaInstitute of Cytology and Genetics, SB RAS, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, RussiaResearch Institute of Medical Genetics, Tomsk NRMC, Tomsk, RussiaSkin fibroblasts from a patient with intellectual disability and ring chromosome 22 were reprogrammed into induced pluripotent stem cells (iPSCs) to establish a clonal stem cell lines, IMGTi001-A (iTAF5-29) and IMGTi001-B (iTAF5-32). Because of ring chromosome mitotic instability these cell lines show mosaic karyotypes with 46,XX,r(22) in >83% cells, 45,XX,-22 as minor class and sporadically cells with other karyotypes. Differentiation in derivatives of all three germ layers was shown in teratoma assay for IMGTi001-A, and in embryoid bodies for both cell lines. To our knowledge, human iPSC lines with ring chromosome are described for the first time.http://www.sciencedirect.com/science/article/pii/S1873506118302046 |
| spellingShingle | T.V. Nikitina A.G. Menzorov A.A. Kashevarova M.M. Gridina A.A. Khabarova Yu.S. Yakovleva M.E. Lopatkina E.A. Kizilova S.A. Vasilyev O.L. Serov I.N. Lebedev Generation of two iPSC lines (IMGTi001-A and IMGTi001-B) from human skin fibroblasts with ring chromosome 22 Stem Cell Research |
| title | Generation of two iPSC lines (IMGTi001-A and IMGTi001-B) from human skin fibroblasts with ring chromosome 22 |
| title_full | Generation of two iPSC lines (IMGTi001-A and IMGTi001-B) from human skin fibroblasts with ring chromosome 22 |
| title_fullStr | Generation of two iPSC lines (IMGTi001-A and IMGTi001-B) from human skin fibroblasts with ring chromosome 22 |
| title_full_unstemmed | Generation of two iPSC lines (IMGTi001-A and IMGTi001-B) from human skin fibroblasts with ring chromosome 22 |
| title_short | Generation of two iPSC lines (IMGTi001-A and IMGTi001-B) from human skin fibroblasts with ring chromosome 22 |
| title_sort | generation of two ipsc lines imgti001 a and imgti001 b from human skin fibroblasts with ring chromosome 22 |
| url | http://www.sciencedirect.com/science/article/pii/S1873506118302046 |
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