Gene expression in diapausing rotifer eggs in response to divergent environmental predictability regimes
Abstract In unpredictable environments in which reliable cues for predicting environmental variation are lacking, a diversifying bet-hedging strategy for diapause exit is expected to evolve, whereby only a portion of diapausing forms will resume development at the first occurrence of suitable condit...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-77727-2 |
| _version_ | 1818997397243559936 |
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| author | Eva Tarazona J. Ignacio Lucas-Lledó María José Carmona Eduardo M. García-Roger |
| author_facet | Eva Tarazona J. Ignacio Lucas-Lledó María José Carmona Eduardo M. García-Roger |
| author_sort | Eva Tarazona |
| collection | DOAJ |
| description | Abstract In unpredictable environments in which reliable cues for predicting environmental variation are lacking, a diversifying bet-hedging strategy for diapause exit is expected to evolve, whereby only a portion of diapausing forms will resume development at the first occurrence of suitable conditions. This study focused on diapause termination in the rotifer Brachionus plicatilis s.s., addressing the transcriptional profile of diapausing eggs from environments differing in the level of predictability and the relationship of such profiles with hatching patterns. RNA-Seq analyses revealed significant differences in gene expression between diapausing eggs produced in the laboratory under combinations of two contrasting selective regimes of environmental fluctuation (predictable vs unpredictable) and two different diapause conditions (passing or not passing through forced diapause). The results showed that the selective regime was more important than the diapause condition in driving differences in the transcriptome profile. Most of the differentially expressed genes were upregulated in the predictable regime and mostly associated with molecular functions involved in embryo morphological development and hatching readiness. This was in concordance with observations of earlier, higher, and more synchronous hatching in diapausing eggs produced under the predictable regime. |
| first_indexed | 2024-12-20T21:44:59Z |
| format | Article |
| id | doaj.art-93453e70565a4e3895e0c11d77aa11f2 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T21:44:59Z |
| publishDate | 2020-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-93453e70565a4e3895e0c11d77aa11f22022-12-21T19:25:43ZengNature PortfolioScientific Reports2045-23222020-12-0110111510.1038/s41598-020-77727-2Gene expression in diapausing rotifer eggs in response to divergent environmental predictability regimesEva Tarazona0J. Ignacio Lucas-Lledó1María José Carmona2Eduardo M. García-Roger3Institut Cavanilles de Biodiversitat I Biologia Evolutiva, Universitat de ValènciaInstitut Cavanilles de Biodiversitat I Biologia Evolutiva, Universitat de ValènciaInstitut Cavanilles de Biodiversitat I Biologia Evolutiva, Universitat de ValènciaInstitut Cavanilles de Biodiversitat I Biologia Evolutiva, Universitat de ValènciaAbstract In unpredictable environments in which reliable cues for predicting environmental variation are lacking, a diversifying bet-hedging strategy for diapause exit is expected to evolve, whereby only a portion of diapausing forms will resume development at the first occurrence of suitable conditions. This study focused on diapause termination in the rotifer Brachionus plicatilis s.s., addressing the transcriptional profile of diapausing eggs from environments differing in the level of predictability and the relationship of such profiles with hatching patterns. RNA-Seq analyses revealed significant differences in gene expression between diapausing eggs produced in the laboratory under combinations of two contrasting selective regimes of environmental fluctuation (predictable vs unpredictable) and two different diapause conditions (passing or not passing through forced diapause). The results showed that the selective regime was more important than the diapause condition in driving differences in the transcriptome profile. Most of the differentially expressed genes were upregulated in the predictable regime and mostly associated with molecular functions involved in embryo morphological development and hatching readiness. This was in concordance with observations of earlier, higher, and more synchronous hatching in diapausing eggs produced under the predictable regime.https://doi.org/10.1038/s41598-020-77727-2 |
| spellingShingle | Eva Tarazona J. Ignacio Lucas-Lledó María José Carmona Eduardo M. García-Roger Gene expression in diapausing rotifer eggs in response to divergent environmental predictability regimes Scientific Reports |
| title | Gene expression in diapausing rotifer eggs in response to divergent environmental predictability regimes |
| title_full | Gene expression in diapausing rotifer eggs in response to divergent environmental predictability regimes |
| title_fullStr | Gene expression in diapausing rotifer eggs in response to divergent environmental predictability regimes |
| title_full_unstemmed | Gene expression in diapausing rotifer eggs in response to divergent environmental predictability regimes |
| title_short | Gene expression in diapausing rotifer eggs in response to divergent environmental predictability regimes |
| title_sort | gene expression in diapausing rotifer eggs in response to divergent environmental predictability regimes |
| url | https://doi.org/10.1038/s41598-020-77727-2 |
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