Compartmentalized oskar degradation in the germ plasm safeguards germline development
Partitioning of mRNAs into ribonucleoprotein (RNP) granules supports diverse regulatory programs within the crowded cytoplasm. At least two types of RNP granules populate the germ plasm, a cytoplasmic domain at the posterior of the Drosophila oocyte and embryo. Germ granules deliver mRNAs required f...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2020-01-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/49988 |
| _version_ | 1811199552393314304 |
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| author | Catherine E Eichler Anna C Hakes Brooke Hull Elizabeth R Gavis |
| author_facet | Catherine E Eichler Anna C Hakes Brooke Hull Elizabeth R Gavis |
| author_sort | Catherine E Eichler |
| collection | DOAJ |
| description | Partitioning of mRNAs into ribonucleoprotein (RNP) granules supports diverse regulatory programs within the crowded cytoplasm. At least two types of RNP granules populate the germ plasm, a cytoplasmic domain at the posterior of the Drosophila oocyte and embryo. Germ granules deliver mRNAs required for germline development to pole cells, the germ cell progenitors. A second type of RNP granule, here named founder granules, contains oskar mRNA, which encodes the germ plasm organizer. Whereas oskar mRNA is essential for germ plasm assembly during oogenesis, we show that it is toxic to pole cells. Founder granules mediate compartmentalized degradation of oskar during embryogenesis to minimize its inheritance by pole cells. Degradation of oskar in founder granules is temporally and mechanistically distinct from degradation of oskar and other mRNAs during the maternal-to-zygotic transition. Our results show how compartmentalization in RNP granules differentially controls fates of mRNAs localized within the same cytoplasmic domain. |
| first_indexed | 2024-04-12T01:50:38Z |
| format | Article |
| id | doaj.art-9346a855510b40a3bafb4c082ecebbe0 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:50:38Z |
| publishDate | 2020-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-9346a855510b40a3bafb4c082ecebbe02022-12-22T03:52:57ZengeLife Sciences Publications LtdeLife2050-084X2020-01-01910.7554/eLife.49988Compartmentalized oskar degradation in the germ plasm safeguards germline developmentCatherine E Eichler0Anna C Hakes1Brooke Hull2Elizabeth R Gavis3https://orcid.org/0000-0003-0251-0760Department of Molecular Biology, Princeton University, Princeton, United StatesDepartment of Molecular Biology, Princeton University, Princeton, United StatesDepartment of Molecular Biology, Princeton University, Princeton, United StatesDepartment of Molecular Biology, Princeton University, Princeton, United StatesPartitioning of mRNAs into ribonucleoprotein (RNP) granules supports diverse regulatory programs within the crowded cytoplasm. At least two types of RNP granules populate the germ plasm, a cytoplasmic domain at the posterior of the Drosophila oocyte and embryo. Germ granules deliver mRNAs required for germline development to pole cells, the germ cell progenitors. A second type of RNP granule, here named founder granules, contains oskar mRNA, which encodes the germ plasm organizer. Whereas oskar mRNA is essential for germ plasm assembly during oogenesis, we show that it is toxic to pole cells. Founder granules mediate compartmentalized degradation of oskar during embryogenesis to minimize its inheritance by pole cells. Degradation of oskar in founder granules is temporally and mechanistically distinct from degradation of oskar and other mRNAs during the maternal-to-zygotic transition. Our results show how compartmentalization in RNP granules differentially controls fates of mRNAs localized within the same cytoplasmic domain.https://elifesciences.org/articles/49988oskargerm plasmmRNA degradationribonucleoproteinRNA granulefounder granule |
| spellingShingle | Catherine E Eichler Anna C Hakes Brooke Hull Elizabeth R Gavis Compartmentalized oskar degradation in the germ plasm safeguards germline development eLife oskar germ plasm mRNA degradation ribonucleoprotein RNA granule founder granule |
| title | Compartmentalized oskar degradation in the germ plasm safeguards germline development |
| title_full | Compartmentalized oskar degradation in the germ plasm safeguards germline development |
| title_fullStr | Compartmentalized oskar degradation in the germ plasm safeguards germline development |
| title_full_unstemmed | Compartmentalized oskar degradation in the germ plasm safeguards germline development |
| title_short | Compartmentalized oskar degradation in the germ plasm safeguards germline development |
| title_sort | compartmentalized oskar degradation in the germ plasm safeguards germline development |
| topic | oskar germ plasm mRNA degradation ribonucleoprotein RNA granule founder granule |
| url | https://elifesciences.org/articles/49988 |
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