Repeated Treatment With the Traditional Medicine Unsei-in Inhibits Substance P-Induced Itch-Associated Responses Through Downregulation of the Expression of Nitric Oxide Synthase 1 in Mice
Unsei-in inhibits substance P (SP)-induced scratching of mice after repeated administration. The involvement of cutaneous nitric oxide (NO) in the SP-induced scratching led us to investigate the effects of Unsei-in on the cutaneous NO system in mice. Seven-day oral administration of Unsei-in (300, b...
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| Format: | Article |
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Elsevier
2004-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319325289 |
| _version_ | 1811263830648422400 |
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| author | Tsugunobu Andoh Ali Al-Akeel Kenichiro Tsujii Hiroshi Nojima Yasushi Kuraishi |
| author_facet | Tsugunobu Andoh Ali Al-Akeel Kenichiro Tsujii Hiroshi Nojima Yasushi Kuraishi |
| author_sort | Tsugunobu Andoh |
| collection | DOAJ |
| description | Unsei-in inhibits substance P (SP)-induced scratching of mice after repeated administration. The involvement of cutaneous nitric oxide (NO) in the SP-induced scratching led us to investigate the effects of Unsei-in on the cutaneous NO system in mice. Seven-day oral administration of Unsei-in (300, but not 100, mg/kg daily) significantly inhibited scratching and the increase of cutaneous NO after intradermal SP injection. The NO synthase 1 (NOS1) inhibitor 7-nitroindazole (1 nmol/site) decreased SP-induced scratching and NO production. Repeated administration of Unsei-in (300 mg/kg) reduced the cutaneous NOS1 level. The results suggest that the inhibition of cutaneous NOS1 expression and NO production participates in the antipruritic action of Unsei-in. Keywords:: Unsei-in, nitric oxide synthase 1, itch-associated response |
| first_indexed | 2024-04-12T19:52:07Z |
| format | Article |
| id | doaj.art-93478f080de740059c58345da675b1d4 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-04-12T19:52:07Z |
| publishDate | 2004-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-93478f080de740059c58345da675b1d42022-12-22T03:18:48ZengElsevierJournal of Pharmacological Sciences1347-86132004-01-01942207210Repeated Treatment With the Traditional Medicine Unsei-in Inhibits Substance P-Induced Itch-Associated Responses Through Downregulation of the Expression of Nitric Oxide Synthase 1 in MiceTsugunobu Andoh0Ali Al-Akeel1Kenichiro Tsujii2Hiroshi Nojima3Yasushi Kuraishi4Department of Applied Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, JapanDepartment of Applied Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, JapanDepartment of Applied Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, JapanDepartment of Applied Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, JapanDepartment of Applied Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan; 21st Century COE Program, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan; Corresponding author (affiliation #1). FAX: +81-76-434-5045 E-mail: kuraisiy@ms.toyama-mpu.ac.jpUnsei-in inhibits substance P (SP)-induced scratching of mice after repeated administration. The involvement of cutaneous nitric oxide (NO) in the SP-induced scratching led us to investigate the effects of Unsei-in on the cutaneous NO system in mice. Seven-day oral administration of Unsei-in (300, but not 100, mg/kg daily) significantly inhibited scratching and the increase of cutaneous NO after intradermal SP injection. The NO synthase 1 (NOS1) inhibitor 7-nitroindazole (1 nmol/site) decreased SP-induced scratching and NO production. Repeated administration of Unsei-in (300 mg/kg) reduced the cutaneous NOS1 level. The results suggest that the inhibition of cutaneous NOS1 expression and NO production participates in the antipruritic action of Unsei-in. Keywords:: Unsei-in, nitric oxide synthase 1, itch-associated responsehttp://www.sciencedirect.com/science/article/pii/S1347861319325289 |
| spellingShingle | Tsugunobu Andoh Ali Al-Akeel Kenichiro Tsujii Hiroshi Nojima Yasushi Kuraishi Repeated Treatment With the Traditional Medicine Unsei-in Inhibits Substance P-Induced Itch-Associated Responses Through Downregulation of the Expression of Nitric Oxide Synthase 1 in Mice Journal of Pharmacological Sciences |
| title | Repeated Treatment With the Traditional Medicine Unsei-in Inhibits Substance P-Induced Itch-Associated Responses Through Downregulation of the Expression of Nitric Oxide Synthase 1 in Mice |
| title_full | Repeated Treatment With the Traditional Medicine Unsei-in Inhibits Substance P-Induced Itch-Associated Responses Through Downregulation of the Expression of Nitric Oxide Synthase 1 in Mice |
| title_fullStr | Repeated Treatment With the Traditional Medicine Unsei-in Inhibits Substance P-Induced Itch-Associated Responses Through Downregulation of the Expression of Nitric Oxide Synthase 1 in Mice |
| title_full_unstemmed | Repeated Treatment With the Traditional Medicine Unsei-in Inhibits Substance P-Induced Itch-Associated Responses Through Downregulation of the Expression of Nitric Oxide Synthase 1 in Mice |
| title_short | Repeated Treatment With the Traditional Medicine Unsei-in Inhibits Substance P-Induced Itch-Associated Responses Through Downregulation of the Expression of Nitric Oxide Synthase 1 in Mice |
| title_sort | repeated treatment with the traditional medicine unsei in inhibits substance p induced itch associated responses through downregulation of the expression of nitric oxide synthase 1 in mice |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319325289 |
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