The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA
Objective:An important criterion that radiopaque poly-3-hydroxy butyrate (PHB) derivatives synthesized and characterized by our study group do not interact with DNA and bovine albumin to be developed as an intravenous contrast agent for radiological imaging.Methods:Interactions of radiopaque PHB der...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2021-04-01
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| Series: | Bezmiâlem Science |
| Subjects: | |
| Online Access: |
http://bezmialemscience.org/archives/archive-detail/article-preview/the-effect-of-odised-poly-3-hydroxy-butyrate-on-dn/47235
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| _version_ | 1797906886674087936 |
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| author | Arzu EROL |
| author_facet | Arzu EROL |
| author_sort | Arzu EROL |
| collection | DOAJ |
| description | Objective:An important criterion that radiopaque poly-3-hydroxy butyrate (PHB) derivatives synthesized and characterized by our study group do not interact with DNA and bovine albumin to be developed as an intravenous contrast agent for radiological imaging.Methods:Interactions of radiopaque PHB derivatives between bovine serum albumin (BSA) and deoxyribonucleic acid (pUC19 DNA and polymerase chain reaction product) were investigated using absorption spectroscopy and agarose gel electrophoresis techniques.Results:Absorption spectroscopy results showed that the radiopaque PHB derivatives did not show any interaction with BSA. Doublestranded annular DNA showed that the interaction between the double-stranded forms of linear DNA did not cause any damage.Conclusion:It was determined that it did not interact with both BSA and various DNA forms. |
| first_indexed | 2024-04-10T10:27:48Z |
| format | Article |
| id | doaj.art-9348be806ae44db3a9336cc6a4ec4274 |
| institution | Directory Open Access Journal |
| issn | 2148-2373 2148-2373 |
| language | English |
| last_indexed | 2024-04-10T10:27:48Z |
| publishDate | 2021-04-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Bezmiâlem Science |
| spelling | doaj.art-9348be806ae44db3a9336cc6a4ec42742023-02-15T16:21:17ZengGalenos Publishing HouseBezmiâlem Science2148-23732148-23732021-04-019213413910.14235/bas.galenos.2020.405613049054The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSAArzu EROL0 Zonguldak Bülent Ecevit University Faculty of Sciences and Arts, Department of Molecular Biology and Genetics, Zonguldak, Turkey Objective:An important criterion that radiopaque poly-3-hydroxy butyrate (PHB) derivatives synthesized and characterized by our study group do not interact with DNA and bovine albumin to be developed as an intravenous contrast agent for radiological imaging.Methods:Interactions of radiopaque PHB derivatives between bovine serum albumin (BSA) and deoxyribonucleic acid (pUC19 DNA and polymerase chain reaction product) were investigated using absorption spectroscopy and agarose gel electrophoresis techniques.Results:Absorption spectroscopy results showed that the radiopaque PHB derivatives did not show any interaction with BSA. Doublestranded annular DNA showed that the interaction between the double-stranded forms of linear DNA did not cause any damage.Conclusion:It was determined that it did not interact with both BSA and various DNA forms. http://bezmialemscience.org/archives/archive-detail/article-preview/the-effect-of-odised-poly-3-hydroxy-butyrate-on-dn/47235 albumindnaiodinated poly-3-hydroxy butyratepuc19 |
| spellingShingle | Arzu EROL The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA Bezmiâlem Science albumin dna iodinated poly-3-hydroxy butyrate puc19 |
| title | The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA |
| title_full | The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA |
| title_fullStr | The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA |
| title_full_unstemmed | The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA |
| title_short | The Effect of Iodised Poly-3-Hydroxy Butyrate on DNA and BSA |
| title_sort | effect of iodised poly 3 hydroxy butyrate on dna and bsa |
| topic | albumin dna iodinated poly-3-hydroxy butyrate puc19 |
| url |
http://bezmialemscience.org/archives/archive-detail/article-preview/the-effect-of-odised-poly-3-hydroxy-butyrate-on-dn/47235
|
| work_keys_str_mv | AT arzuerol theeffectofiodisedpoly3hydroxybutyrateondnaandbsa AT arzuerol effectofiodisedpoly3hydroxybutyrateondnaandbsa |