Clinical Correlation of Bladder Electron Microscopic Characteristics in Patients with Detrusor Underactivity of Various Etiologies
This study aimed to investigate the ultrastructural characteristics of the bladder of patients with detrusor underactivity (DU) of various etiologies. Twenty-five patients with DU and control subjects underwent urodynamic testing and transmission electron microscopic examination of bladder specimens...
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MDPI AG
2022-05-01
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| Online Access: | https://www.mdpi.com/2227-9059/10/5/1055 |
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| author | Jia-Fong Jhang Han-Chen Ho Yuan-Hong Jiang Yung-Hsiang Hsu Hann-Chorng Kuo |
| author_facet | Jia-Fong Jhang Han-Chen Ho Yuan-Hong Jiang Yung-Hsiang Hsu Hann-Chorng Kuo |
| author_sort | Jia-Fong Jhang |
| collection | DOAJ |
| description | This study aimed to investigate the ultrastructural characteristics of the bladder of patients with detrusor underactivity (DU) of various etiologies. Twenty-five patients with DU and control subjects underwent urodynamic testing and transmission electron microscopic examination of bladder specimens. The epithelium, lamina propria, and muscle layers were analyzed separately. The DU bladders exhibited total epithelial denudation (52%). In the bladders with remaining epithelium, apical cell uroplakins (44.4%) and tight junction complexes (77.8%) were also noted. The lamina propria was characterized by loose extracellular connective tissue (48%) and a lack of nerve terminals (76%). Smooth muscle shrinkage and a loss of their regular spindle shape (91.6%) were also noted in the detrusor layer. Patients with DU with intact epithelial cell layers had significantly larger void volumes and maximal flow rates than those with mild or severe epithelial denudation. Patients with remaining nerve terminals in lamina propria had a stronger first sensation of filling and smaller residual urine volume than those without nerve terminals. The proportion of ultrastructural defects of the bladder was not significantly different among patients with DU of various etiologies and treatment outcomes. DU bladders were characterized by ultrastructural defects in the entire bladder, and the defects were correlated to clinical parameters. |
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| format | Article |
| id | doaj.art-934a677ba6fa49b8bdc5749b468b6f71 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T03:17:38Z |
| publishDate | 2022-05-01 |
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| spelling | doaj.art-934a677ba6fa49b8bdc5749b468b6f712023-11-23T10:10:24ZengMDPI AGBiomedicines2227-90592022-05-01105105510.3390/biomedicines10051055Clinical Correlation of Bladder Electron Microscopic Characteristics in Patients with Detrusor Underactivity of Various EtiologiesJia-Fong Jhang0Han-Chen Ho1Yuan-Hong Jiang2Yung-Hsiang Hsu3Hann-Chorng Kuo4Department of Urology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Tzu Chi University, Hualien 970, TaiwanDepartment of Anatomy, Tzu Chi University, Hualien 970, TaiwanDepartment of Urology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Tzu Chi University, Hualien 970, TaiwanDepartment of Pathology, Buddhist Tzu Chi General Hospital, Tzu Chi University, Hualien 970, TaiwanDepartment of Urology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Tzu Chi University, Hualien 970, TaiwanThis study aimed to investigate the ultrastructural characteristics of the bladder of patients with detrusor underactivity (DU) of various etiologies. Twenty-five patients with DU and control subjects underwent urodynamic testing and transmission electron microscopic examination of bladder specimens. The epithelium, lamina propria, and muscle layers were analyzed separately. The DU bladders exhibited total epithelial denudation (52%). In the bladders with remaining epithelium, apical cell uroplakins (44.4%) and tight junction complexes (77.8%) were also noted. The lamina propria was characterized by loose extracellular connective tissue (48%) and a lack of nerve terminals (76%). Smooth muscle shrinkage and a loss of their regular spindle shape (91.6%) were also noted in the detrusor layer. Patients with DU with intact epithelial cell layers had significantly larger void volumes and maximal flow rates than those with mild or severe epithelial denudation. Patients with remaining nerve terminals in lamina propria had a stronger first sensation of filling and smaller residual urine volume than those without nerve terminals. The proportion of ultrastructural defects of the bladder was not significantly different among patients with DU of various etiologies and treatment outcomes. DU bladders were characterized by ultrastructural defects in the entire bladder, and the defects were correlated to clinical parameters.https://www.mdpi.com/2227-9059/10/5/1055urodynamicurine retentionpathogenesistreatment outcome |
| spellingShingle | Jia-Fong Jhang Han-Chen Ho Yuan-Hong Jiang Yung-Hsiang Hsu Hann-Chorng Kuo Clinical Correlation of Bladder Electron Microscopic Characteristics in Patients with Detrusor Underactivity of Various Etiologies Biomedicines urodynamic urine retention pathogenesis treatment outcome |
| title | Clinical Correlation of Bladder Electron Microscopic Characteristics in Patients with Detrusor Underactivity of Various Etiologies |
| title_full | Clinical Correlation of Bladder Electron Microscopic Characteristics in Patients with Detrusor Underactivity of Various Etiologies |
| title_fullStr | Clinical Correlation of Bladder Electron Microscopic Characteristics in Patients with Detrusor Underactivity of Various Etiologies |
| title_full_unstemmed | Clinical Correlation of Bladder Electron Microscopic Characteristics in Patients with Detrusor Underactivity of Various Etiologies |
| title_short | Clinical Correlation of Bladder Electron Microscopic Characteristics in Patients with Detrusor Underactivity of Various Etiologies |
| title_sort | clinical correlation of bladder electron microscopic characteristics in patients with detrusor underactivity of various etiologies |
| topic | urodynamic urine retention pathogenesis treatment outcome |
| url | https://www.mdpi.com/2227-9059/10/5/1055 |
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