Review of CRISPR/Cas Systems on Detection of Nucleotide Sequences
Nowadays, with the rapid development of biotechnology, the CRISPR/Cas technology in particular has produced many new traits and products. Therefore, rapid and high-resolution detection methods for biotechnology products are urgently needed, which is extremely important for safety regulation. Recentl...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/12/3/477 |
| _version_ | 1827760064285376512 |
|---|---|
| author | Mengyu Wang Haoqian Wang Kai Li Xiaoman Li Xujing Wang Zhixing Wang |
| author_facet | Mengyu Wang Haoqian Wang Kai Li Xiaoman Li Xujing Wang Zhixing Wang |
| author_sort | Mengyu Wang |
| collection | DOAJ |
| description | Nowadays, with the rapid development of biotechnology, the CRISPR/Cas technology in particular has produced many new traits and products. Therefore, rapid and high-resolution detection methods for biotechnology products are urgently needed, which is extremely important for safety regulation. Recently, in addition to being gene editing tools, CRISPR/Cas systems have also been used in detection of various targets. CRISPR/Cas systems can be successfully used to detect nucleic acids, proteins, metal ions and others in combination with a variety of technologies, with great application prospects in the future. However, there are still some challenges need to be addressed. In this review, we will list some detection methods of genetically modified (GM) crops, gene-edited crops and single-nucleotide polymorphisms (SNPs) based on CRISPR/Cas systems, hoping to bring some inspiration or ideas to readers. |
| first_indexed | 2024-03-11T09:45:07Z |
| format | Article |
| id | doaj.art-934fe76220e84b82ad276f2cfe2c7eee |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T09:45:07Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-934fe76220e84b82ad276f2cfe2c7eee2023-11-16T16:39:56ZengMDPI AGFoods2304-81582023-01-0112347710.3390/foods12030477Review of CRISPR/Cas Systems on Detection of Nucleotide SequencesMengyu Wang0Haoqian Wang1Kai Li2Xiaoman Li3Xujing Wang4Zhixing Wang5Key Laboratory on Safety Assessment (Molecular) of Agri-GMO, Ministry of Agriculture and Rural Affairs, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaDevelopment Center for Science and Technology, Ministry of Agriculture and Rural Affairs, Beijing 100176, ChinaInstitute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory on Safety Assessment (Molecular) of Agri-GMO, Ministry of Agriculture and Rural Affairs, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory on Safety Assessment (Molecular) of Agri-GMO, Ministry of Agriculture and Rural Affairs, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory on Safety Assessment (Molecular) of Agri-GMO, Ministry of Agriculture and Rural Affairs, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaNowadays, with the rapid development of biotechnology, the CRISPR/Cas technology in particular has produced many new traits and products. Therefore, rapid and high-resolution detection methods for biotechnology products are urgently needed, which is extremely important for safety regulation. Recently, in addition to being gene editing tools, CRISPR/Cas systems have also been used in detection of various targets. CRISPR/Cas systems can be successfully used to detect nucleic acids, proteins, metal ions and others in combination with a variety of technologies, with great application prospects in the future. However, there are still some challenges need to be addressed. In this review, we will list some detection methods of genetically modified (GM) crops, gene-edited crops and single-nucleotide polymorphisms (SNPs) based on CRISPR/Cas systems, hoping to bring some inspiration or ideas to readers.https://www.mdpi.com/2304-8158/12/3/477CRISPR/Cas-based detection<i>trans</i>-cleavagegenetically modified organisms detectiongene-edited products detectionsingle-nucleotide polymorphisms detectionchallenges and prospects |
| spellingShingle | Mengyu Wang Haoqian Wang Kai Li Xiaoman Li Xujing Wang Zhixing Wang Review of CRISPR/Cas Systems on Detection of Nucleotide Sequences Foods CRISPR/Cas-based detection <i>trans</i>-cleavage genetically modified organisms detection gene-edited products detection single-nucleotide polymorphisms detection challenges and prospects |
| title | Review of CRISPR/Cas Systems on Detection of Nucleotide Sequences |
| title_full | Review of CRISPR/Cas Systems on Detection of Nucleotide Sequences |
| title_fullStr | Review of CRISPR/Cas Systems on Detection of Nucleotide Sequences |
| title_full_unstemmed | Review of CRISPR/Cas Systems on Detection of Nucleotide Sequences |
| title_short | Review of CRISPR/Cas Systems on Detection of Nucleotide Sequences |
| title_sort | review of crispr cas systems on detection of nucleotide sequences |
| topic | CRISPR/Cas-based detection <i>trans</i>-cleavage genetically modified organisms detection gene-edited products detection single-nucleotide polymorphisms detection challenges and prospects |
| url | https://www.mdpi.com/2304-8158/12/3/477 |
| work_keys_str_mv | AT mengyuwang reviewofcrisprcassystemsondetectionofnucleotidesequences AT haoqianwang reviewofcrisprcassystemsondetectionofnucleotidesequences AT kaili reviewofcrisprcassystemsondetectionofnucleotidesequences AT xiaomanli reviewofcrisprcassystemsondetectionofnucleotidesequences AT xujingwang reviewofcrisprcassystemsondetectionofnucleotidesequences AT zhixingwang reviewofcrisprcassystemsondetectionofnucleotidesequences |