IRE1 knockdown modifies hypoxic regulation of cathepsins and LONP1 genes expression in U87 glioma cells
We have studied the effect of hypoxia on the expression of genes encoding for LONP1/PRSS15 and cathepsins in U87 glioma cells in relation to inhibition of inositol requiring enzyme-1 (IRE1). It was shown that hypoxia up-regulated the expression of CTSA, CTSB, CTSD, CTSF, CTSK, and LONP1 genes and do...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2017-04-01
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| Series: | The Ukrainian Biochemical Journal |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2017/04/Minchenko-2_2_17.pdf |
| _version_ | 1797422315709923328 |
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| author | O. H. Minchenko О. O. Riabovol O. V. Halkin D. O. Minchenko О. O. Ratushna |
| author_facet | O. H. Minchenko О. O. Riabovol O. V. Halkin D. O. Minchenko О. O. Ratushna |
| author_sort | O. H. Minchenko |
| collection | DOAJ |
| description | We have studied the effect of hypoxia on the expression of genes encoding for LONP1/PRSS15 and cathepsins in U87 glioma cells in relation to inhibition of inositol requiring enzyme-1 (IRE1). It was shown that hypoxia up-regulated the expression of CTSA, CTSB, CTSD, CTSF, CTSK, and LONP1 genes and down-regulated the expression of CTSC, CTSL, CTSO, and CTSS genes in control glioma cells (transfected by empty vector). Inhibition of ІRE1 signaling enzyme function in these glioma cells modified effect of hypoxia on the expression of most studied genes: eliminated the effect of hypoxia on CTSA, and LONP1 genes, introduced reverse changes on CTSD and CTSS genes, reduced – on CTSF and CTSK genes, and enhanced – on CTSB and CTSL genes. Therefore, hypoxia affected the expression level of most studied genes in relation to the functional activity of IRE1 enzyme, a central mediator of endoplasmic reticulum stress, which control cell proliferation and tumor growth. |
| first_indexed | 2024-03-09T07:30:26Z |
| format | Article |
| id | doaj.art-93511c23925244a89aa8795dad1b493f |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-09T07:30:26Z |
| publishDate | 2017-04-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-93511c23925244a89aa8795dad1b493f2023-12-03T06:26:37ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032017-04-01892556910.15407/ubj89.02.055IRE1 knockdown modifies hypoxic regulation of cathepsins and LONP1 genes expression in U87 glioma cellsO. H. Minchenko0О. O. Riabovol1O. V. Halkin2D. O. Minchenko3О. O. Ratushna4Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv; Bohomolets National Medical University, Kyiv, UkrainePalladin Institute of Biochemistry, National Academy of Sciences of Ukraine, KyivWe have studied the effect of hypoxia on the expression of genes encoding for LONP1/PRSS15 and cathepsins in U87 glioma cells in relation to inhibition of inositol requiring enzyme-1 (IRE1). It was shown that hypoxia up-regulated the expression of CTSA, CTSB, CTSD, CTSF, CTSK, and LONP1 genes and down-regulated the expression of CTSC, CTSL, CTSO, and CTSS genes in control glioma cells (transfected by empty vector). Inhibition of ІRE1 signaling enzyme function in these glioma cells modified effect of hypoxia on the expression of most studied genes: eliminated the effect of hypoxia on CTSA, and LONP1 genes, introduced reverse changes on CTSD and CTSS genes, reduced – on CTSF and CTSK genes, and enhanced – on CTSB and CTSL genes. Therefore, hypoxia affected the expression level of most studied genes in relation to the functional activity of IRE1 enzyme, a central mediator of endoplasmic reticulum stress, which control cell proliferation and tumor growth.http://ukrbiochemjournal.org/wp-content/uploads/2017/04/Minchenko-2_2_17.pdf |
| spellingShingle | O. H. Minchenko О. O. Riabovol O. V. Halkin D. O. Minchenko О. O. Ratushna IRE1 knockdown modifies hypoxic regulation of cathepsins and LONP1 genes expression in U87 glioma cells The Ukrainian Biochemical Journal |
| title | IRE1 knockdown modifies hypoxic regulation of cathepsins and LONP1 genes expression in U87 glioma cells |
| title_full | IRE1 knockdown modifies hypoxic regulation of cathepsins and LONP1 genes expression in U87 glioma cells |
| title_fullStr | IRE1 knockdown modifies hypoxic regulation of cathepsins and LONP1 genes expression in U87 glioma cells |
| title_full_unstemmed | IRE1 knockdown modifies hypoxic regulation of cathepsins and LONP1 genes expression in U87 glioma cells |
| title_short | IRE1 knockdown modifies hypoxic regulation of cathepsins and LONP1 genes expression in U87 glioma cells |
| title_sort | ire1 knockdown modifies hypoxic regulation of cathepsins and lonp1 genes expression in u87 glioma cells |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2017/04/Minchenko-2_2_17.pdf |
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