Methanotroph (Methylococcus capsulatus, Bath) as an alternative protein source for genetically improved farmed tilapia (GIFT: Oreochromis niloticus) and its effect on antioxidants and immune response
The aim was to evaluate the effects of increasing bacterial protein meal (BPM) (M. capsulatus, Bath) as a fishmeal replacer in the diets of GIFT. A total of 540 juveniles (initial weight 3 g) were allocated randomly to 18 outdoor floating cages (1 m 1 m 1 m) at 30 fish/cage for 56 days with diets (0...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-11-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S235251342100288X |
| _version_ | 1819240825511477248 |
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| author | Majory Kabwe Hamunjo Chama Hualiang Liang Dongyu Huang Xianping Ge Mingchun Ren Lu Zhang Longhua Wu Ji Ke |
| author_facet | Majory Kabwe Hamunjo Chama Hualiang Liang Dongyu Huang Xianping Ge Mingchun Ren Lu Zhang Longhua Wu Ji Ke |
| author_sort | Majory Kabwe Hamunjo Chama |
| collection | DOAJ |
| description | The aim was to evaluate the effects of increasing bacterial protein meal (BPM) (M. capsulatus, Bath) as a fishmeal replacer in the diets of GIFT. A total of 540 juveniles (initial weight 3 g) were allocated randomly to 18 outdoor floating cages (1 m 1 m 1 m) at 30 fish/cage for 56 days with diets (0, 17, 34, 51, 68 and 85 g/kg) prepared in triplicate. Weight gain rate, specific growth rate, feed intake, feed conversation ratio, and survival rate were not significantly affected across all diets (P > 0.05). Immunoglobulin M and albumin were significantly increased with an increase in BPM, while alanine transaminases, aspartate transaminases, and alkaline phosphatase showed no significant difference (P > 0.05). The activities of catalase, superoxide dismutase, glutathione peroxidase and glutathione showed no significant difference (P > 0.05). Meanwhile, total antioxidant capacity increased significantly, while malondialdehyde showed an opposite trend (P < 0.05). Expressions of nuclear factor erythroid 2-related factor 2, glutathione peroxide - 1 mRNA and total superoxide dismutase mRNA showed no significantly difference (P > 0.05). However, nuclear factor-kappa B, tumor necrosis factor-α, interfelony, interleukin 8, interleukin 10 and interleukin 16 decreased significantly (P < 0.05). Nonetheless interleukin 1β remained unchanged (P > 0.05). In conclusion, our study has demonstrated that M. capsulatus Bath could improve the immune response without affecting the growth and antioxidant capacity in GIFT juveniles. |
| first_indexed | 2024-12-23T14:14:10Z |
| format | Article |
| id | doaj.art-935585a298904ca3b44c7fd330b32942 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-12-23T14:14:10Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-935585a298904ca3b44c7fd330b329422022-12-21T17:43:58ZengElsevierAquaculture Reports2352-51342021-11-0121100872Methanotroph (Methylococcus capsulatus, Bath) as an alternative protein source for genetically improved farmed tilapia (GIFT: Oreochromis niloticus) and its effect on antioxidants and immune responseMajory Kabwe Hamunjo Chama0Hualiang Liang1Dongyu Huang2Xianping Ge3Mingchun Ren4Lu Zhang5Longhua Wu6Ji Ke7Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; Natural Resources Development College, P/Bag CH99, Lusaka, ZambiaKey Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology, Freshwater Fisheries Research Center (FFRC), Chinese Academy of Fishery Sciences (CAFS), Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; Key Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology, Freshwater Fisheries Research Center (FFRC), Chinese Academy of Fishery Sciences (CAFS), Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; Key Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology, Freshwater Fisheries Research Center (FFRC), Chinese Academy of Fishery Sciences (CAFS), Wuxi 214081, China; Corresponding author at: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.Tongwei Co., Ltd., Healthy Aquaculture Key Laboratory of Sichuan Province, Chengdu 610093, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaThe aim was to evaluate the effects of increasing bacterial protein meal (BPM) (M. capsulatus, Bath) as a fishmeal replacer in the diets of GIFT. A total of 540 juveniles (initial weight 3 g) were allocated randomly to 18 outdoor floating cages (1 m 1 m 1 m) at 30 fish/cage for 56 days with diets (0, 17, 34, 51, 68 and 85 g/kg) prepared in triplicate. Weight gain rate, specific growth rate, feed intake, feed conversation ratio, and survival rate were not significantly affected across all diets (P > 0.05). Immunoglobulin M and albumin were significantly increased with an increase in BPM, while alanine transaminases, aspartate transaminases, and alkaline phosphatase showed no significant difference (P > 0.05). The activities of catalase, superoxide dismutase, glutathione peroxidase and glutathione showed no significant difference (P > 0.05). Meanwhile, total antioxidant capacity increased significantly, while malondialdehyde showed an opposite trend (P < 0.05). Expressions of nuclear factor erythroid 2-related factor 2, glutathione peroxide - 1 mRNA and total superoxide dismutase mRNA showed no significantly difference (P > 0.05). However, nuclear factor-kappa B, tumor necrosis factor-α, interfelony, interleukin 8, interleukin 10 and interleukin 16 decreased significantly (P < 0.05). Nonetheless interleukin 1β remained unchanged (P > 0.05). In conclusion, our study has demonstrated that M. capsulatus Bath could improve the immune response without affecting the growth and antioxidant capacity in GIFT juveniles.http://www.sciencedirect.com/science/article/pii/S235251342100288XGIFT (Oreochromis niloticus)Methanotroph (M. capsulatus, Bath) proteinFish meal replacementAntioxidants statusImmune response |
| spellingShingle | Majory Kabwe Hamunjo Chama Hualiang Liang Dongyu Huang Xianping Ge Mingchun Ren Lu Zhang Longhua Wu Ji Ke Methanotroph (Methylococcus capsulatus, Bath) as an alternative protein source for genetically improved farmed tilapia (GIFT: Oreochromis niloticus) and its effect on antioxidants and immune response Aquaculture Reports GIFT (Oreochromis niloticus) Methanotroph (M. capsulatus, Bath) protein Fish meal replacement Antioxidants status Immune response |
| title | Methanotroph (Methylococcus capsulatus, Bath) as an alternative protein source for genetically improved farmed tilapia (GIFT: Oreochromis niloticus) and its effect on antioxidants and immune response |
| title_full | Methanotroph (Methylococcus capsulatus, Bath) as an alternative protein source for genetically improved farmed tilapia (GIFT: Oreochromis niloticus) and its effect on antioxidants and immune response |
| title_fullStr | Methanotroph (Methylococcus capsulatus, Bath) as an alternative protein source for genetically improved farmed tilapia (GIFT: Oreochromis niloticus) and its effect on antioxidants and immune response |
| title_full_unstemmed | Methanotroph (Methylococcus capsulatus, Bath) as an alternative protein source for genetically improved farmed tilapia (GIFT: Oreochromis niloticus) and its effect on antioxidants and immune response |
| title_short | Methanotroph (Methylococcus capsulatus, Bath) as an alternative protein source for genetically improved farmed tilapia (GIFT: Oreochromis niloticus) and its effect on antioxidants and immune response |
| title_sort | methanotroph methylococcus capsulatus bath as an alternative protein source for genetically improved farmed tilapia gift oreochromis niloticus and its effect on antioxidants and immune response |
| topic | GIFT (Oreochromis niloticus) Methanotroph (M. capsulatus, Bath) protein Fish meal replacement Antioxidants status Immune response |
| url | http://www.sciencedirect.com/science/article/pii/S235251342100288X |
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