The Association between Single Nucleotide Polymorphisms rs1042522 and rs1642785 in the TP53 gene and Acute Myeloid leukemia in a sample of the Baghdad/ Iraq population
Acute myeloid leukemia (AML) represents the most prevalent type of acute leukemia in adults and is responsible for approximately 80% of all cases. The tumor suppressor gene (TP53) is a gene that has been frequently studied in cancer, and mutations in this gene account for about 50% of human cancers...
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| Format: | Article |
| Language: | Arabic |
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College of Science for Women, University of Baghdad
2024-03-01
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| Series: | Baghdad Science Journal |
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| Online Access: | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/8207 |
| _version_ | 1797289128352546816 |
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| author | Hadeel Hameed Dawood Rana Kadhim Mohammed |
| author_facet | Hadeel Hameed Dawood Rana Kadhim Mohammed |
| author_sort | Hadeel Hameed Dawood |
| collection | DOAJ |
| description |
Acute myeloid leukemia (AML) represents the most prevalent type of acute leukemia in adults and is responsible for approximately 80% of all cases. The tumor suppressor gene (TP53) is a gene that has been frequently studied in cancer, and mutations in this gene account for about 50% of human cancers. This study aims to evaluate the correlation between two single nucleotide polymorphisms (SNPs) in the gene: rs1042522 and rs1642785, and a group of Iraqi patients suffering from pre-diagnostic acute myeloid leukemia (AML). Blood samples were collected from sixty patients (26 males and 34 females) and sixty controls (26 males and 34 females); these subjects were matched in gender, age, and ethnicity. Genomic DNA has been extracted from whole frozen blood samples by using the Easy Pure® Blood Genomic Kit, and then the purity and concentration have been measured by using the Nano drop NAS-99 spectrophotometer. Nano Drop readings ranged between 7-55ng/µl for the concentration and between 1.78-1.9 for the purity. High resolution melt (HRM) real-time PCR was used in the detection of these two SNPs. TP53 genotype frequencies have been in accordance with Hardy–Weinberg equilibrium (HWE), with statistically significant differences p≤0.05 between the genotypes of the control and patient groups. The rs1042522 genotype frequency was significantly different between patients and controls p = 0.0001, and participants with the GA genotype were more likely to develop AML OR = 7.8, 95% CI 3.2–18.4, p= 0.0001. In addition, the genotype frequency of rs1642785 was significantly different between patients and controls p = 0.002, and participants with the GA genotype were more likely to develop AML OR = 3.5, 95% CI 1.5–8.12, p = 0.002.
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| first_indexed | 2024-03-07T18:59:34Z |
| format | Article |
| id | doaj.art-9355f6f2b5114bf999b9e232b8177370 |
| institution | Directory Open Access Journal |
| issn | 2078-8665 2411-7986 |
| language | Arabic |
| last_indexed | 2024-03-07T18:59:34Z |
| publishDate | 2024-03-01 |
| publisher | College of Science for Women, University of Baghdad |
| record_format | Article |
| series | Baghdad Science Journal |
| spelling | doaj.art-9355f6f2b5114bf999b9e232b81773702024-03-01T20:06:35ZaraCollege of Science for Women, University of BaghdadBaghdad Science Journal2078-86652411-79862024-03-0121310.21123/bsj.2023.8207The Association between Single Nucleotide Polymorphisms rs1042522 and rs1642785 in the TP53 gene and Acute Myeloid leukemia in a sample of the Baghdad/ Iraq populationHadeel Hameed Dawood0Rana Kadhim Mohammed 1Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq.Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq. Acute myeloid leukemia (AML) represents the most prevalent type of acute leukemia in adults and is responsible for approximately 80% of all cases. The tumor suppressor gene (TP53) is a gene that has been frequently studied in cancer, and mutations in this gene account for about 50% of human cancers. This study aims to evaluate the correlation between two single nucleotide polymorphisms (SNPs) in the gene: rs1042522 and rs1642785, and a group of Iraqi patients suffering from pre-diagnostic acute myeloid leukemia (AML). Blood samples were collected from sixty patients (26 males and 34 females) and sixty controls (26 males and 34 females); these subjects were matched in gender, age, and ethnicity. Genomic DNA has been extracted from whole frozen blood samples by using the Easy Pure® Blood Genomic Kit, and then the purity and concentration have been measured by using the Nano drop NAS-99 spectrophotometer. Nano Drop readings ranged between 7-55ng/µl for the concentration and between 1.78-1.9 for the purity. High resolution melt (HRM) real-time PCR was used in the detection of these two SNPs. TP53 genotype frequencies have been in accordance with Hardy–Weinberg equilibrium (HWE), with statistically significant differences p≤0.05 between the genotypes of the control and patient groups. The rs1042522 genotype frequency was significantly different between patients and controls p = 0.0001, and participants with the GA genotype were more likely to develop AML OR = 7.8, 95% CI 3.2–18.4, p= 0.0001. In addition, the genotype frequency of rs1642785 was significantly different between patients and controls p = 0.002, and participants with the GA genotype were more likely to develop AML OR = 3.5, 95% CI 1.5–8.12, p = 0.002. https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/8207Acute myeloid leukemia, HRM-qPCR, rs1042522, rs1642785, Single Nucleotide Polymorphisms, Tumor protein 53 (TP53) |
| spellingShingle | Hadeel Hameed Dawood Rana Kadhim Mohammed The Association between Single Nucleotide Polymorphisms rs1042522 and rs1642785 in the TP53 gene and Acute Myeloid leukemia in a sample of the Baghdad/ Iraq population Baghdad Science Journal Acute myeloid leukemia, HRM-qPCR, rs1042522, rs1642785, Single Nucleotide Polymorphisms, Tumor protein 53 (TP53) |
| title | The Association between Single Nucleotide Polymorphisms rs1042522 and rs1642785 in the TP53 gene and Acute Myeloid leukemia in a sample of the Baghdad/ Iraq population |
| title_full | The Association between Single Nucleotide Polymorphisms rs1042522 and rs1642785 in the TP53 gene and Acute Myeloid leukemia in a sample of the Baghdad/ Iraq population |
| title_fullStr | The Association between Single Nucleotide Polymorphisms rs1042522 and rs1642785 in the TP53 gene and Acute Myeloid leukemia in a sample of the Baghdad/ Iraq population |
| title_full_unstemmed | The Association between Single Nucleotide Polymorphisms rs1042522 and rs1642785 in the TP53 gene and Acute Myeloid leukemia in a sample of the Baghdad/ Iraq population |
| title_short | The Association between Single Nucleotide Polymorphisms rs1042522 and rs1642785 in the TP53 gene and Acute Myeloid leukemia in a sample of the Baghdad/ Iraq population |
| title_sort | association between single nucleotide polymorphisms rs1042522 and rs1642785 in the tp53 gene and acute myeloid leukemia in a sample of the baghdad iraq population |
| topic | Acute myeloid leukemia, HRM-qPCR, rs1042522, rs1642785, Single Nucleotide Polymorphisms, Tumor protein 53 (TP53) |
| url | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/8207 |
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