Cytochrome c adsorption on various poly-L-glutamic acid-containing calcium phosphate particles
We synthesized novel materials at different temperatures with two types of poly-L-glutamic acid––poly-α-glutamic acid and poly-γ-glutamic acid––to confirm the effect of poly-L-glutamic acid, which exhibits hydroxyapatite (HAp) (pGlu-HAp) crystallinity, on particle morphologies and cytochrome c (Cyt...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-07-01
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| Series: | Open Ceramics |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666539520300092 |
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| author | Suzuka Kojima Sungho Lee Fukue Nagata Katsuya Kato |
| author_facet | Suzuka Kojima Sungho Lee Fukue Nagata Katsuya Kato |
| author_sort | Suzuka Kojima |
| collection | DOAJ |
| description | We synthesized novel materials at different temperatures with two types of poly-L-glutamic acid––poly-α-glutamic acid and poly-γ-glutamic acid––to confirm the effect of poly-L-glutamic acid, which exhibits hydroxyapatite (HAp) (pGlu-HAp) crystallinity, on particle morphologies and cytochrome c (Cyt c) adsorption abilities. Through hydrothermal treatment, rod-like particles, as well as thin sheet-like particles, grew along the c-axis. Moreover, the crystallinity of particles was found to improve with an increase in the reaction temperature from 60 °C to 120 °C. The Cyt c adsorption ability of pGlu-HAp, rather than the crystallinity of calcium phosphate, was affected by the peptide structure. Furthermore, pGlu–HAp was used effectively for Cyt c adsorption with not only the carboxyl residues of side chains in peptides but also mesopores in the range of 3–30 nm. In addition, the catalytic activity of Cyt c adsorbed on the particles containing poly-α-glutamic acid retained high activity and was similar to that exhibited by native free Cyt c. The graphene oxide/imogolite/Cyt c@pGlu-HAp-modified glassy carbon electrode showed good electrocatalytic responses as a candidate for a H2O2 biosensor. According to these results, the pGlu-HAp materials prepared in this study can act as useful enzyme carriers and electrodes, because they maintain high enzyme activity when adsorbed on particles. |
| first_indexed | 2024-12-13T11:12:19Z |
| format | Article |
| id | doaj.art-9359bd55a0d64076abc0c0a3f931c8e0 |
| institution | Directory Open Access Journal |
| issn | 2666-5395 |
| language | English |
| last_indexed | 2024-12-13T11:12:19Z |
| publishDate | 2020-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Open Ceramics |
| spelling | doaj.art-9359bd55a0d64076abc0c0a3f931c8e02022-12-21T23:48:42ZengElsevierOpen Ceramics2666-53952020-07-012100009Cytochrome c adsorption on various poly-L-glutamic acid-containing calcium phosphate particlesSuzuka Kojima0Sungho Lee1Fukue Nagata2Katsuya Kato3National Institute of Advanced Industrial Science and Technology 2266-98, Anagahora, Shimo-Shidami, Moriyamaku, Nagoya, Aichi, 463-8560, JapanNational Institute of Advanced Industrial Science and Technology 2266-98, Anagahora, Shimo-Shidami, Moriyamaku, Nagoya, Aichi, 463-8560, JapanNational Institute of Advanced Industrial Science and Technology 2266-98, Anagahora, Shimo-Shidami, Moriyamaku, Nagoya, Aichi, 463-8560, JapanCorresponding author.; National Institute of Advanced Industrial Science and Technology 2266-98, Anagahora, Shimo-Shidami, Moriyamaku, Nagoya, Aichi, 463-8560, JapanWe synthesized novel materials at different temperatures with two types of poly-L-glutamic acid––poly-α-glutamic acid and poly-γ-glutamic acid––to confirm the effect of poly-L-glutamic acid, which exhibits hydroxyapatite (HAp) (pGlu-HAp) crystallinity, on particle morphologies and cytochrome c (Cyt c) adsorption abilities. Through hydrothermal treatment, rod-like particles, as well as thin sheet-like particles, grew along the c-axis. Moreover, the crystallinity of particles was found to improve with an increase in the reaction temperature from 60 °C to 120 °C. The Cyt c adsorption ability of pGlu-HAp, rather than the crystallinity of calcium phosphate, was affected by the peptide structure. Furthermore, pGlu–HAp was used effectively for Cyt c adsorption with not only the carboxyl residues of side chains in peptides but also mesopores in the range of 3–30 nm. In addition, the catalytic activity of Cyt c adsorbed on the particles containing poly-α-glutamic acid retained high activity and was similar to that exhibited by native free Cyt c. The graphene oxide/imogolite/Cyt c@pGlu-HAp-modified glassy carbon electrode showed good electrocatalytic responses as a candidate for a H2O2 biosensor. According to these results, the pGlu-HAp materials prepared in this study can act as useful enzyme carriers and electrodes, because they maintain high enzyme activity when adsorbed on particles.http://www.sciencedirect.com/science/article/pii/S2666539520300092HydroxyapatitePoly-L-glutamic acidCrystallinityPorous structureProtein adsorptionCytochrome c |
| spellingShingle | Suzuka Kojima Sungho Lee Fukue Nagata Katsuya Kato Cytochrome c adsorption on various poly-L-glutamic acid-containing calcium phosphate particles Open Ceramics Hydroxyapatite Poly-L-glutamic acid Crystallinity Porous structure Protein adsorption Cytochrome c |
| title | Cytochrome c adsorption on various poly-L-glutamic acid-containing calcium phosphate particles |
| title_full | Cytochrome c adsorption on various poly-L-glutamic acid-containing calcium phosphate particles |
| title_fullStr | Cytochrome c adsorption on various poly-L-glutamic acid-containing calcium phosphate particles |
| title_full_unstemmed | Cytochrome c adsorption on various poly-L-glutamic acid-containing calcium phosphate particles |
| title_short | Cytochrome c adsorption on various poly-L-glutamic acid-containing calcium phosphate particles |
| title_sort | cytochrome c adsorption on various poly l glutamic acid containing calcium phosphate particles |
| topic | Hydroxyapatite Poly-L-glutamic acid Crystallinity Porous structure Protein adsorption Cytochrome c |
| url | http://www.sciencedirect.com/science/article/pii/S2666539520300092 |
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