HtrA Protein Hyperproduction Increases the Viability of Bacillus subtilis Cells under the Stress Conditions and Stimulates Biofilm Formation
HtrA (high-temperature requirement A) are the heat shock-induced serine proteases widely spread in pro- and eukaryotic cells. These enzymes control the quality of intracellular proteins by hydrolysis of denatured proteins, thereby protecting cells from various stresses. In many pathogenic bacteria,...
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| Format: | Article |
| Language: | English |
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Kazan Federal University
2017-06-01
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| Series: | Učënye Zapiski Kazanskogo Universiteta. Seriâ Estestvennye Nauki |
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| Online Access: | http://kpfu.ru/portal/docs/F1870477354/159_2_est_6.pdf |
| _version_ | 1818294396696657920 |
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| author | L.S. Chernova I.S. Sharafutdinov A.R. Kayumov |
| author_facet | L.S. Chernova I.S. Sharafutdinov A.R. Kayumov |
| author_sort | L.S. Chernova |
| collection | DOAJ |
| description | HtrA (high-temperature requirement A) are the heat shock-induced serine proteases widely spread in pro- and eukaryotic cells. These enzymes control the quality of intracellular proteins by hydrolysis of denatured proteins, thereby protecting cells from various stresses. In many pathogenic bacteria, they are factors of pathogenicity. Thus, in Streptococcus mutans, HtrA participates in quorum-dependent processes and biofilm formation via digestion of the pheromone ComX. In this work, Bacillus subtilis HtrA Hy with an overexpressionof the HtrA protein has been obtained and its physiological features have been characterized. The overexpression of HtrA in Bacillus subtilis cells increases cell viability at high temperature conditions, ethanol and salt stresses. In addition, B. subtilis HtrA Hy is characterized by the intensive biofilm formation with an increased synthesis of extracellular matrix. Congo red staining of the co lonyhas revealed that HtrA overexpression induces the increased production of amyloid-like proteins. Moreover, these cells exhibited intensive swarming in contrast to the wild-type strain. The overexpression of the HtrA protease results in an increased level of expression of the eps and yqxM genes encoding the components of the biofilm matrix. This fact is probably related to the effect of the enzyme on regulatory processes, possibly due to the hydrolysis of signal regulatory peptides. |
| first_indexed | 2024-12-13T03:31:05Z |
| format | Article |
| id | doaj.art-9359cc71b7c2437ea57a165ca3bb556e |
| institution | Directory Open Access Journal |
| issn | 2542-064X 2500-218X |
| language | English |
| last_indexed | 2024-12-13T03:31:05Z |
| publishDate | 2017-06-01 |
| publisher | Kazan Federal University |
| record_format | Article |
| series | Učënye Zapiski Kazanskogo Universiteta. Seriâ Estestvennye Nauki |
| spelling | doaj.art-9359cc71b7c2437ea57a165ca3bb556e2022-12-22T00:01:10ZengKazan Federal UniversityUčënye Zapiski Kazanskogo Universiteta. Seriâ Estestvennye Nauki2542-064X2500-218X2017-06-011592262271HtrA Protein Hyperproduction Increases the Viability of Bacillus subtilis Cells under the Stress Conditions and Stimulates Biofilm FormationL.S. Chernova0I.S. Sharafutdinov1A.R. Kayumov2Kazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaKazan Federal University, Kazan, 420008 RussiaHtrA (high-temperature requirement A) are the heat shock-induced serine proteases widely spread in pro- and eukaryotic cells. These enzymes control the quality of intracellular proteins by hydrolysis of denatured proteins, thereby protecting cells from various stresses. In many pathogenic bacteria, they are factors of pathogenicity. Thus, in Streptococcus mutans, HtrA participates in quorum-dependent processes and biofilm formation via digestion of the pheromone ComX. In this work, Bacillus subtilis HtrA Hy with an overexpressionof the HtrA protein has been obtained and its physiological features have been characterized. The overexpression of HtrA in Bacillus subtilis cells increases cell viability at high temperature conditions, ethanol and salt stresses. In addition, B. subtilis HtrA Hy is characterized by the intensive biofilm formation with an increased synthesis of extracellular matrix. Congo red staining of the co lonyhas revealed that HtrA overexpression induces the increased production of amyloid-like proteins. Moreover, these cells exhibited intensive swarming in contrast to the wild-type strain. The overexpression of the HtrA protease results in an increased level of expression of the eps and yqxM genes encoding the components of the biofilm matrix. This fact is probably related to the effect of the enzyme on regulatory processes, possibly due to the hydrolysis of signal regulatory peptides.http://kpfu.ru/portal/docs/F1870477354/159_2_est_6.pdfprotease HtrAbiofilm formationviabilitytemperature stressprotease hyperproduction |
| spellingShingle | L.S. Chernova I.S. Sharafutdinov A.R. Kayumov HtrA Protein Hyperproduction Increases the Viability of Bacillus subtilis Cells under the Stress Conditions and Stimulates Biofilm Formation Učënye Zapiski Kazanskogo Universiteta. Seriâ Estestvennye Nauki protease HtrA biofilm formation viability temperature stress protease hyperproduction |
| title | HtrA Protein Hyperproduction Increases the Viability of Bacillus subtilis Cells under the Stress Conditions and Stimulates Biofilm Formation |
| title_full | HtrA Protein Hyperproduction Increases the Viability of Bacillus subtilis Cells under the Stress Conditions and Stimulates Biofilm Formation |
| title_fullStr | HtrA Protein Hyperproduction Increases the Viability of Bacillus subtilis Cells under the Stress Conditions and Stimulates Biofilm Formation |
| title_full_unstemmed | HtrA Protein Hyperproduction Increases the Viability of Bacillus subtilis Cells under the Stress Conditions and Stimulates Biofilm Formation |
| title_short | HtrA Protein Hyperproduction Increases the Viability of Bacillus subtilis Cells under the Stress Conditions and Stimulates Biofilm Formation |
| title_sort | htra protein hyperproduction increases the viability of bacillus subtilis cells under the stress conditions and stimulates biofilm formation |
| topic | protease HtrA biofilm formation viability temperature stress protease hyperproduction |
| url | http://kpfu.ru/portal/docs/F1870477354/159_2_est_6.pdf |
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