miR-423-5p Inhibits Osteosarcoma Proliferation and Invasion Through Directly Targeting STMN1

Background/Aims: Increasing evidences suggest that dysregulated expression of miRNAs contributes to the progression of various tumors. However, the underlying function of miR-423-5p in osteosarcoma remains unexplored. Methods: The expression of miR-423-5p and STMN1 were determined in osteosarcoma sa...

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Main Authors: Xuesong Wang, Lei Peng, Xiaojin Gong, Xiugong Zhang, Ruifu Sun, Jinlong Du
Format: Article
Language:English
Published: Cell Physiol Biochem Press GmbH & Co KG 2018-11-01
Series:Cellular Physiology and Biochemistry
Subjects:
Online Access:https://www.karger.com/Article/FullText/495085
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author Xuesong Wang
Lei Peng
Xiaojin Gong
Xiugong Zhang
Ruifu Sun
Jinlong Du
author_facet Xuesong Wang
Lei Peng
Xiaojin Gong
Xiugong Zhang
Ruifu Sun
Jinlong Du
author_sort Xuesong Wang
collection DOAJ
description Background/Aims: Increasing evidences suggest that dysregulated expression of miRNAs contributes to the progression of various tumors. However, the underlying function of miR-423-5p in osteosarcoma remains unexplored. Methods: The expression of miR-423-5p and STMN1 were determined in osteosarcoma samples and cell lines via quantitative real-time PCR. Colony formation and Cell Counting Kit-8 (CCK-8) assays were performed to measure cell proliferation ability and transwell analysis was used to detect cell invasion, and dual luciferase reporter assay was perform to analysis the interaction between the miR-423-5p and STMN1. Results: The expression levels of miR-423-5p and STMN1 in the osteosarcoma tissues and cell lines were measured by qRT-PCR. Cell viability was determined using the clone formation and CCK-8 assays. A dual-luciferase reporter and Western blot were performed to stdudy the target gene of miR-423-5p. Here, we showed that miR-423-5p expression was downregulated in osteosarcoma tissues and cell lines. However, the expression of stathmin1 (STMN1) was downregulated in osteosarcoma tissues and cell lines. Moreover, STMN1 expression level was negatively correlated with the miR-423-5p expression in the osteosarcoma tissues. We identified STMN1 was a direct target gene of miR-423-5p in osteosarcoma cell. Overexpression of miR-423-5p inhibited osteosarcoma cell proliferation, colony formation and invasion. Furthermore, we demonstrated that STMN1 was involved in miR-423-5p-mediated cell behavior such as cell proliferation, colony formation and invasion in the osteosarcoma cell. Conclusion: Our present study indicated that miR-423-5p acted as a tumor suppressor gene in osteosarcoma partly through inhibiting STMN1 expression.
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spelling doaj.art-935b1990143e468f881d103b4c5ae5bc2022-12-21T19:56:34ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782018-11-015062249225910.1159/000495085495085miR-423-5p Inhibits Osteosarcoma Proliferation and Invasion Through Directly Targeting STMN1Xuesong WangLei PengXiaojin GongXiugong ZhangRuifu SunJinlong DuBackground/Aims: Increasing evidences suggest that dysregulated expression of miRNAs contributes to the progression of various tumors. However, the underlying function of miR-423-5p in osteosarcoma remains unexplored. Methods: The expression of miR-423-5p and STMN1 were determined in osteosarcoma samples and cell lines via quantitative real-time PCR. Colony formation and Cell Counting Kit-8 (CCK-8) assays were performed to measure cell proliferation ability and transwell analysis was used to detect cell invasion, and dual luciferase reporter assay was perform to analysis the interaction between the miR-423-5p and STMN1. Results: The expression levels of miR-423-5p and STMN1 in the osteosarcoma tissues and cell lines were measured by qRT-PCR. Cell viability was determined using the clone formation and CCK-8 assays. A dual-luciferase reporter and Western blot were performed to stdudy the target gene of miR-423-5p. Here, we showed that miR-423-5p expression was downregulated in osteosarcoma tissues and cell lines. However, the expression of stathmin1 (STMN1) was downregulated in osteosarcoma tissues and cell lines. Moreover, STMN1 expression level was negatively correlated with the miR-423-5p expression in the osteosarcoma tissues. We identified STMN1 was a direct target gene of miR-423-5p in osteosarcoma cell. Overexpression of miR-423-5p inhibited osteosarcoma cell proliferation, colony formation and invasion. Furthermore, we demonstrated that STMN1 was involved in miR-423-5p-mediated cell behavior such as cell proliferation, colony formation and invasion in the osteosarcoma cell. Conclusion: Our present study indicated that miR-423-5p acted as a tumor suppressor gene in osteosarcoma partly through inhibiting STMN1 expression.https://www.karger.com/Article/FullText/495085OsteosarcomaMicroRNAsmiR-423-5pSTMN1
spellingShingle Xuesong Wang
Lei Peng
Xiaojin Gong
Xiugong Zhang
Ruifu Sun
Jinlong Du
miR-423-5p Inhibits Osteosarcoma Proliferation and Invasion Through Directly Targeting STMN1
Cellular Physiology and Biochemistry
Osteosarcoma
MicroRNAs
miR-423-5p
STMN1
title miR-423-5p Inhibits Osteosarcoma Proliferation and Invasion Through Directly Targeting STMN1
title_full miR-423-5p Inhibits Osteosarcoma Proliferation and Invasion Through Directly Targeting STMN1
title_fullStr miR-423-5p Inhibits Osteosarcoma Proliferation and Invasion Through Directly Targeting STMN1
title_full_unstemmed miR-423-5p Inhibits Osteosarcoma Proliferation and Invasion Through Directly Targeting STMN1
title_short miR-423-5p Inhibits Osteosarcoma Proliferation and Invasion Through Directly Targeting STMN1
title_sort mir 423 5p inhibits osteosarcoma proliferation and invasion through directly targeting stmn1
topic Osteosarcoma
MicroRNAs
miR-423-5p
STMN1
url https://www.karger.com/Article/FullText/495085
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