The rearranged mitochondrial genome of Leptopilina boulardi (Hymenoptera: Figitidae), a parasitoid wasp of Drosophila
Abstract The partial mitochondrial genome sequence of Leptopilina boulardi (Hymenoptera: Figitidae) was characterized. Illumina sequencing was used yielding 35,999,679 reads, from which 102,482 were utilized in the assembly. The length of the sequenced region of this partial mitochondrial genome is...
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| Language: | English |
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Sociedade Brasileira de Genética
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| Series: | Genetics and Molecular Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400611&lng=en&tlng=en |
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| author | Daniel S. Oliveira Tiago M.F.F. Gomes Elgion L.S. Loreto |
| author_facet | Daniel S. Oliveira Tiago M.F.F. Gomes Elgion L.S. Loreto |
| author_sort | Daniel S. Oliveira |
| collection | DOAJ |
| description | Abstract The partial mitochondrial genome sequence of Leptopilina boulardi (Hymenoptera: Figitidae) was characterized. Illumina sequencing was used yielding 35,999,679 reads, from which 102,482 were utilized in the assembly. The length of the sequenced region of this partial mitochondrial genome is 15,417 bp, consisting of 13 protein-coding, two rRNA, and 21tRNA genes (the trnaM failed to be sequenced) and a partial A+T-rich region. All protein-coding genes start with ATN codons. Eleven protein-coding genes presented TAA stop codons, whereas ND6 and COII that presented TA, and T nucleotides, respectively. The gene pattern revealed extensive rearrangements compared to the typical pattern generally observed in insects. These rearrangements involve two protein-coding and two ribosomal genes, along with the 16 tRNA genes. This gene order is different from the pattern described for Ibalia leucospoides (Ibaliidae, Cynipoidea), suggesting that this particular gene order can be variable among Cynipoidea superfamily members. A maximum likelihood phylogenetic analysis of the main groups of Apocrita was performed using amino acid sequence of 13 protein-coding genes, showing monophyly for the Cynipoidea superfamily within the Hymenoptera phylogeny. |
| first_indexed | 2024-12-20T10:43:19Z |
| format | Article |
| id | doaj.art-93621f39281e4fb8bb85b6a344492471 |
| institution | Directory Open Access Journal |
| issn | 1678-4685 |
| language | English |
| last_indexed | 2024-12-20T10:43:19Z |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-93621f39281e4fb8bb85b6a3444924712022-12-21T19:43:30ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-468539461161510.1590/1678-4685-gmb-2016-0062S1415-47572016000400611The rearranged mitochondrial genome of Leptopilina boulardi (Hymenoptera: Figitidae), a parasitoid wasp of DrosophilaDaniel S. OliveiraTiago M.F.F. GomesElgion L.S. LoretoAbstract The partial mitochondrial genome sequence of Leptopilina boulardi (Hymenoptera: Figitidae) was characterized. Illumina sequencing was used yielding 35,999,679 reads, from which 102,482 were utilized in the assembly. The length of the sequenced region of this partial mitochondrial genome is 15,417 bp, consisting of 13 protein-coding, two rRNA, and 21tRNA genes (the trnaM failed to be sequenced) and a partial A+T-rich region. All protein-coding genes start with ATN codons. Eleven protein-coding genes presented TAA stop codons, whereas ND6 and COII that presented TA, and T nucleotides, respectively. The gene pattern revealed extensive rearrangements compared to the typical pattern generally observed in insects. These rearrangements involve two protein-coding and two ribosomal genes, along with the 16 tRNA genes. This gene order is different from the pattern described for Ibalia leucospoides (Ibaliidae, Cynipoidea), suggesting that this particular gene order can be variable among Cynipoidea superfamily members. A maximum likelihood phylogenetic analysis of the main groups of Apocrita was performed using amino acid sequence of 13 protein-coding genes, showing monophyly for the Cynipoidea superfamily within the Hymenoptera phylogeny.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400611&lng=en&tlng=enMitogenomeCynipoideaLeptopilinaparasitic wasp |
| spellingShingle | Daniel S. Oliveira Tiago M.F.F. Gomes Elgion L.S. Loreto The rearranged mitochondrial genome of Leptopilina boulardi (Hymenoptera: Figitidae), a parasitoid wasp of Drosophila Genetics and Molecular Biology Mitogenome Cynipoidea Leptopilina parasitic wasp |
| title | The rearranged mitochondrial genome of Leptopilina boulardi (Hymenoptera: Figitidae), a parasitoid wasp of Drosophila |
| title_full | The rearranged mitochondrial genome of Leptopilina boulardi (Hymenoptera: Figitidae), a parasitoid wasp of Drosophila |
| title_fullStr | The rearranged mitochondrial genome of Leptopilina boulardi (Hymenoptera: Figitidae), a parasitoid wasp of Drosophila |
| title_full_unstemmed | The rearranged mitochondrial genome of Leptopilina boulardi (Hymenoptera: Figitidae), a parasitoid wasp of Drosophila |
| title_short | The rearranged mitochondrial genome of Leptopilina boulardi (Hymenoptera: Figitidae), a parasitoid wasp of Drosophila |
| title_sort | rearranged mitochondrial genome of leptopilina boulardi hymenoptera figitidae a parasitoid wasp of drosophila |
| topic | Mitogenome Cynipoidea Leptopilina parasitic wasp |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400611&lng=en&tlng=en |
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