Metabolomics of bronchoalveolar lavage in children with persistent wheezing
Abstract Background Recent studies have demonstrated the important role of metabolomics in the pathogenesis of asthma. However, the role of lung metabolomics in childhood persistent wheezing (PW) or wheezing recurrence remains poorly understood. Methods In this prospective observational study, we pe...
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| Format: | Article |
| Language: | English |
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BMC
2022-06-01
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| Series: | Respiratory Research |
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| Online Access: | https://doi.org/10.1186/s12931-022-02087-6 |
| _version_ | 1811230006830956544 |
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| author | Lingfang Liang Minfei Hu Yuanling Chen Lingke Liu Lei Wu Chengcheng Hang Xiaofei Luo Xuefeng Xu |
| author_facet | Lingfang Liang Minfei Hu Yuanling Chen Lingke Liu Lei Wu Chengcheng Hang Xiaofei Luo Xuefeng Xu |
| author_sort | Lingfang Liang |
| collection | DOAJ |
| description | Abstract Background Recent studies have demonstrated the important role of metabolomics in the pathogenesis of asthma. However, the role of lung metabolomics in childhood persistent wheezing (PW) or wheezing recurrence remains poorly understood. Methods In this prospective observational study, we performed a liquid chromatography/mass spectrometry-based metabolomic survey on bronchoalveolar lavage samples collected from 30 children with PW and 30 age-matched infants (control group). A 2-year follow-up study on these PW children was conducted. Results Children with PW showed a distinct characterization of respiratory metabolome compared with control group. Children with PW had higher abundances of choline, oleamide, nepetalactam, butyrylcarnitine, l-palmitoylcarnitine, palmitoylethanolamide, and various phosphatidylcholines. The glycerophospholipid metabolism pathway was the most relevant pathway involving in PW pathophysiologic process. Additionally, different gender, prematurity, and systemic corticoids use demonstrated a greater impact in airway metabolite compositions. Furthermore, for PW children with recurrence during the follow-up period, children who were born prematurely had an increased abundance of butyrylcarnitine relative to those who were carried to term. Conclusions This study suggests that the alterations of lung metabolites could be associated with the development of wheezing, and this early alteration could also be correlated with wheezing recurrence later in life. |
| first_indexed | 2024-04-12T10:21:39Z |
| format | Article |
| id | doaj.art-9362eff021954eb0be5563498732387b |
| institution | Directory Open Access Journal |
| issn | 1465-993X |
| language | English |
| last_indexed | 2024-04-12T10:21:39Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-9362eff021954eb0be5563498732387b2022-12-22T03:37:04ZengBMCRespiratory Research1465-993X2022-06-0123111010.1186/s12931-022-02087-6Metabolomics of bronchoalveolar lavage in children with persistent wheezingLingfang Liang0Minfei Hu1Yuanling Chen2Lingke Liu3Lei Wu4Chengcheng Hang5Xiaofei Luo6Xuefeng Xu7Department of Rheumatology Immunology and Allergy, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child HealthDepartment of Rheumatology Immunology and Allergy, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child HealthDepartment of Rheumatology Immunology and Allergy, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child HealthDepartment of Rheumatology Immunology and Allergy, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child HealthDepartment of Rheumatology Immunology and Allergy, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child HealthDepartment of Rheumatology Immunology and Allergy, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child HealthDepartment of Rheumatology Immunology and Allergy, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child HealthDepartment of Rheumatology Immunology and Allergy, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child HealthAbstract Background Recent studies have demonstrated the important role of metabolomics in the pathogenesis of asthma. However, the role of lung metabolomics in childhood persistent wheezing (PW) or wheezing recurrence remains poorly understood. Methods In this prospective observational study, we performed a liquid chromatography/mass spectrometry-based metabolomic survey on bronchoalveolar lavage samples collected from 30 children with PW and 30 age-matched infants (control group). A 2-year follow-up study on these PW children was conducted. Results Children with PW showed a distinct characterization of respiratory metabolome compared with control group. Children with PW had higher abundances of choline, oleamide, nepetalactam, butyrylcarnitine, l-palmitoylcarnitine, palmitoylethanolamide, and various phosphatidylcholines. The glycerophospholipid metabolism pathway was the most relevant pathway involving in PW pathophysiologic process. Additionally, different gender, prematurity, and systemic corticoids use demonstrated a greater impact in airway metabolite compositions. Furthermore, for PW children with recurrence during the follow-up period, children who were born prematurely had an increased abundance of butyrylcarnitine relative to those who were carried to term. Conclusions This study suggests that the alterations of lung metabolites could be associated with the development of wheezing, and this early alteration could also be correlated with wheezing recurrence later in life.https://doi.org/10.1186/s12931-022-02087-6AirwayBronchoalveolar lavageChildrenMetabolomicsPersistent wheezing |
| spellingShingle | Lingfang Liang Minfei Hu Yuanling Chen Lingke Liu Lei Wu Chengcheng Hang Xiaofei Luo Xuefeng Xu Metabolomics of bronchoalveolar lavage in children with persistent wheezing Respiratory Research Airway Bronchoalveolar lavage Children Metabolomics Persistent wheezing |
| title | Metabolomics of bronchoalveolar lavage in children with persistent wheezing |
| title_full | Metabolomics of bronchoalveolar lavage in children with persistent wheezing |
| title_fullStr | Metabolomics of bronchoalveolar lavage in children with persistent wheezing |
| title_full_unstemmed | Metabolomics of bronchoalveolar lavage in children with persistent wheezing |
| title_short | Metabolomics of bronchoalveolar lavage in children with persistent wheezing |
| title_sort | metabolomics of bronchoalveolar lavage in children with persistent wheezing |
| topic | Airway Bronchoalveolar lavage Children Metabolomics Persistent wheezing |
| url | https://doi.org/10.1186/s12931-022-02087-6 |
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