Protocol for studying co-infection between SARS-CoV-2 and Staphylococcus aureus in vitro
Summary: Bacterial co-infection is one of the most common complications of SARS CoV-2 infection. Here, we present a protocol for the in vitro study of co-infection between SARS CoV-2 and Staphylococcus aureus. We describe steps for quantifying viral and bacterial replication kinetics in the same sam...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723003787 |
| _version_ | 1797789229172916224 |
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| author | Mariya I. Goncheva David E. Heinrichs |
| author_facet | Mariya I. Goncheva David E. Heinrichs |
| author_sort | Mariya I. Goncheva |
| collection | DOAJ |
| description | Summary: Bacterial co-infection is one of the most common complications of SARS CoV-2 infection. Here, we present a protocol for the in vitro study of co-infection between SARS CoV-2 and Staphylococcus aureus. We describe steps for quantifying viral and bacterial replication kinetics in the same sample, with the optional extraction of host RNA and proteins. This protocol is applicable to many viral and bacterial strains and can be performed in different cell types.For complete details on the use and execution of this protocol, please refer to Goncheva et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-13T01:47:44Z |
| format | Article |
| id | doaj.art-9363417819b5416abd4e7743f2f225c2 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-13T01:47:44Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-9363417819b5416abd4e7743f2f225c22023-07-03T04:04:40ZengElsevierSTAR Protocols2666-16672023-09-0143102411Protocol for studying co-infection between SARS-CoV-2 and Staphylococcus aureus in vitroMariya I. Goncheva0David E. Heinrichs1Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC V8P 5C2, Canada; Corresponding authorDepartment of Microbiology and Immunology, University of Western Ontario, London, ON N6A 5C1, Canada; Corresponding authorSummary: Bacterial co-infection is one of the most common complications of SARS CoV-2 infection. Here, we present a protocol for the in vitro study of co-infection between SARS CoV-2 and Staphylococcus aureus. We describe steps for quantifying viral and bacterial replication kinetics in the same sample, with the optional extraction of host RNA and proteins. This protocol is applicable to many viral and bacterial strains and can be performed in different cell types.For complete details on the use and execution of this protocol, please refer to Goncheva et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723003787Cell CultureMicrobiologyMolecular Biology |
| spellingShingle | Mariya I. Goncheva David E. Heinrichs Protocol for studying co-infection between SARS-CoV-2 and Staphylococcus aureus in vitro STAR Protocols Cell Culture Microbiology Molecular Biology |
| title | Protocol for studying co-infection between SARS-CoV-2 and Staphylococcus aureus in vitro |
| title_full | Protocol for studying co-infection between SARS-CoV-2 and Staphylococcus aureus in vitro |
| title_fullStr | Protocol for studying co-infection between SARS-CoV-2 and Staphylococcus aureus in vitro |
| title_full_unstemmed | Protocol for studying co-infection between SARS-CoV-2 and Staphylococcus aureus in vitro |
| title_short | Protocol for studying co-infection between SARS-CoV-2 and Staphylococcus aureus in vitro |
| title_sort | protocol for studying co infection between sars cov 2 and staphylococcus aureus in vitro |
| topic | Cell Culture Microbiology Molecular Biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723003787 |
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