Genetic Diversity in Oilseed and Vegetable Mustard (<i>Brassica juncea</i> L.) Accessions Revealed by Nuclear and Mitochondrial Molecular Markers
Genetic diversity analysis is a fundamental work for effective management and utilization of plant germplasm. <i>Brassica juncea</i> is an economically important crop, including both oilseed and vegetable types. In the present study, a total of 99 accessions of Brassicaceae family, inclu...
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MDPI AG
2023-03-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/3/919 |
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| author | Dongsuo Zhang Haibo Yu Lianliang Gao Jing Wang Hui Dong Yuan Guo Shengwu Hu |
| author_facet | Dongsuo Zhang Haibo Yu Lianliang Gao Jing Wang Hui Dong Yuan Guo Shengwu Hu |
| author_sort | Dongsuo Zhang |
| collection | DOAJ |
| description | Genetic diversity analysis is a fundamental work for effective management and utilization of plant germplasm. <i>Brassica juncea</i> is an economically important crop, including both oilseed and vegetable types. In the present study, a total of 99 accessions of Brassicaceae family, including 84 mustard (50 oilseed and 34 vegetable types) in China and 15 other Brassicaceae accessions were evaluated for their genetic diversity using nuclear and mitochondrial molecular markers. All accessions were evaluated using 18 simple sequence repeats, 20 sequence related amplified polymorphisms, and 7 intron-exon splice junction primers, and in total, 232 polymorphic fragments were obtained. The unweighted pair-group method with arithmetic mean cluster analysis indicated that all accessions could be divided into three major clusters, with cluster I including all 50 oilseed mustard, cluster II including 34 vegetable mustard, and cluster III containing 15 other Brassicaceae accessions. The results of principal component analysis and population structure analysis were in accordance with the cluster result. Molecular variance analysis revealed that the genetic variation was 34.07% among populations and 65.93% within <i>Brassica</i> species, which indicates existence of considerable genetic variation among oilseed and vegetable <i>B</i>. <i>juncea</i> species. Based on an InDel and a SNP locus reported in <i>B</i>. <i>juncea</i> mitochondrial genome, all the 84 <i>B</i>. <i>juncea</i> mitochondrial genomes were divided into three mitotypes (MTs1-3), 22 accessions of MT1, 20 accessions of MT2, and 42 accessions of MT3. In addition, the results of the modified multiplex PCR, Indel and SNP could identify <i>pol</i>-, <i>cam</i>-, <i>nap-</i> (or MT4), <i>Bol</i>-, <i>Bni</i>-, <i>Esa</i>-, and <i>In</i>-cytoplasmic types in 15 other Brassicaceae accessions. Together, oilseed and vegetable <i>B. juncea</i> can be used for broadening the genetic background for each other. |
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| spelling | doaj.art-9364d593f3b44e878a88985f6e6c110e2023-11-17T09:08:10ZengMDPI AGAgronomy2073-43952023-03-0113391910.3390/agronomy13030919Genetic Diversity in Oilseed and Vegetable Mustard (<i>Brassica juncea</i> L.) Accessions Revealed by Nuclear and Mitochondrial Molecular MarkersDongsuo Zhang0Haibo Yu1Lianliang Gao2Jing Wang3Hui Dong4Yuan Guo5Shengwu Hu6State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaGenetic diversity analysis is a fundamental work for effective management and utilization of plant germplasm. <i>Brassica juncea</i> is an economically important crop, including both oilseed and vegetable types. In the present study, a total of 99 accessions of Brassicaceae family, including 84 mustard (50 oilseed and 34 vegetable types) in China and 15 other Brassicaceae accessions were evaluated for their genetic diversity using nuclear and mitochondrial molecular markers. All accessions were evaluated using 18 simple sequence repeats, 20 sequence related amplified polymorphisms, and 7 intron-exon splice junction primers, and in total, 232 polymorphic fragments were obtained. The unweighted pair-group method with arithmetic mean cluster analysis indicated that all accessions could be divided into three major clusters, with cluster I including all 50 oilseed mustard, cluster II including 34 vegetable mustard, and cluster III containing 15 other Brassicaceae accessions. The results of principal component analysis and population structure analysis were in accordance with the cluster result. Molecular variance analysis revealed that the genetic variation was 34.07% among populations and 65.93% within <i>Brassica</i> species, which indicates existence of considerable genetic variation among oilseed and vegetable <i>B</i>. <i>juncea</i> species. Based on an InDel and a SNP locus reported in <i>B</i>. <i>juncea</i> mitochondrial genome, all the 84 <i>B</i>. <i>juncea</i> mitochondrial genomes were divided into three mitotypes (MTs1-3), 22 accessions of MT1, 20 accessions of MT2, and 42 accessions of MT3. In addition, the results of the modified multiplex PCR, Indel and SNP could identify <i>pol</i>-, <i>cam</i>-, <i>nap-</i> (or MT4), <i>Bol</i>-, <i>Bni</i>-, <i>Esa</i>-, and <i>In</i>-cytoplasmic types in 15 other Brassicaceae accessions. Together, oilseed and vegetable <i>B. juncea</i> can be used for broadening the genetic background for each other.https://www.mdpi.com/2073-4395/13/3/919<i>Brassica juncea</i> L.genetic variationsimple sequence repeatsequence related amplified polymorphismintron-exon splice junction markersInDel |
| spellingShingle | Dongsuo Zhang Haibo Yu Lianliang Gao Jing Wang Hui Dong Yuan Guo Shengwu Hu Genetic Diversity in Oilseed and Vegetable Mustard (<i>Brassica juncea</i> L.) Accessions Revealed by Nuclear and Mitochondrial Molecular Markers Agronomy <i>Brassica juncea</i> L. genetic variation simple sequence repeat sequence related amplified polymorphism intron-exon splice junction markers InDel |
| title | Genetic Diversity in Oilseed and Vegetable Mustard (<i>Brassica juncea</i> L.) Accessions Revealed by Nuclear and Mitochondrial Molecular Markers |
| title_full | Genetic Diversity in Oilseed and Vegetable Mustard (<i>Brassica juncea</i> L.) Accessions Revealed by Nuclear and Mitochondrial Molecular Markers |
| title_fullStr | Genetic Diversity in Oilseed and Vegetable Mustard (<i>Brassica juncea</i> L.) Accessions Revealed by Nuclear and Mitochondrial Molecular Markers |
| title_full_unstemmed | Genetic Diversity in Oilseed and Vegetable Mustard (<i>Brassica juncea</i> L.) Accessions Revealed by Nuclear and Mitochondrial Molecular Markers |
| title_short | Genetic Diversity in Oilseed and Vegetable Mustard (<i>Brassica juncea</i> L.) Accessions Revealed by Nuclear and Mitochondrial Molecular Markers |
| title_sort | genetic diversity in oilseed and vegetable mustard i brassica juncea i l accessions revealed by nuclear and mitochondrial molecular markers |
| topic | <i>Brassica juncea</i> L. genetic variation simple sequence repeat sequence related amplified polymorphism intron-exon splice junction markers InDel |
| url | https://www.mdpi.com/2073-4395/13/3/919 |
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