The Bioactive Compound Contents and Potential Protective Effects of Royal Jelly Protein Hydrolysates against DNA Oxidative Damage and LDL Oxidation
In this study, the inhibition of DNA oxidative damage and low-density lipoprotein (LDL) oxidation of royal jelly protein (RJP) hydrolysates obtained from two commercial proteases were investigated. The results showed that the inhibition of DNA oxidative damage induced by the Fenton reaction, RJP, RJ...
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MDPI AG
2021-04-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/4/580 |
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| author | Shu-Hua Chiang Kia-Min Yang Shiann-Cherng Sheu Chih-Wei Chen |
| author_facet | Shu-Hua Chiang Kia-Min Yang Shiann-Cherng Sheu Chih-Wei Chen |
| author_sort | Shu-Hua Chiang |
| collection | DOAJ |
| description | In this study, the inhibition of DNA oxidative damage and low-density lipoprotein (LDL) oxidation of royal jelly protein (RJP) hydrolysates obtained from two commercial proteases were investigated. The results showed that the inhibition of DNA oxidative damage induced by the Fenton reaction, RJP, RJPs hydrolyzed by alcalase (RJP-A), RJPs hydrolyzed by flavourzyme (RPJ-F) and RJP two-stage hydrolysates (RPJ-AF) all had the effect of inhibiting deoxyribose oxidative damage. The inhibition effect of RJP, RJP-A, RJP-F and RJP-AF (1.0 mg/mL) were 47.06%, 33.70%, 24.19% and 43.09%, respectively. In addition, studies have also found that both RJP and RJP hydrolysates can reduce the production of 8-OH-2′-dG and the order of its inhibitory ability is RJP-AF ≒ RJP-A > RJP-F > RJP. The inhibition of DNA damage induced by bleomycin-Fe<sup>3+</sup>/ascorbic acid (Asc) with the addition of RJP, RJP-A, RPJ-F and RPJ-AF were 17.16%, 30.88%, 25.00% and 37.25%, respectively. The results of LDL oxidation inhibition showed that RJP-AF (1 mg/mL) not only had the most effective inhibitory Cu<sup>2+</sup>-induced LDL oxidation to produce a thiobarbituric acid reactive substance (TBARS) but also extended the lag time of conjugated diene formation to 300 min, which was 3.3 times that of the control group. |
| first_indexed | 2024-03-10T12:29:09Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T12:29:09Z |
| publishDate | 2021-04-01 |
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| series | Antioxidants |
| spelling | doaj.art-936557ef17434b48ad0bda9a316a61c42023-11-21T14:49:00ZengMDPI AGAntioxidants2076-39212021-04-0110458010.3390/antiox10040580The Bioactive Compound Contents and Potential Protective Effects of Royal Jelly Protein Hydrolysates against DNA Oxidative Damage and LDL OxidationShu-Hua Chiang0Kia-Min Yang1Shiann-Cherng Sheu2Chih-Wei Chen3Department of Health and Creative Vegetarian Science, Fo Guang University, Yilan 26247, TaiwanDepartment of Hospitality Management, Mingdao University, Changhua 523, TaiwanBachelor Degree Program in Environment and Food Safety Laboratory Science, Chang Jung Christian University, Tainan 71101, TaiwanBachelor Degree Program in Environment and Food Safety Laboratory Science, Chang Jung Christian University, Tainan 71101, TaiwanIn this study, the inhibition of DNA oxidative damage and low-density lipoprotein (LDL) oxidation of royal jelly protein (RJP) hydrolysates obtained from two commercial proteases were investigated. The results showed that the inhibition of DNA oxidative damage induced by the Fenton reaction, RJP, RJPs hydrolyzed by alcalase (RJP-A), RJPs hydrolyzed by flavourzyme (RPJ-F) and RJP two-stage hydrolysates (RPJ-AF) all had the effect of inhibiting deoxyribose oxidative damage. The inhibition effect of RJP, RJP-A, RJP-F and RJP-AF (1.0 mg/mL) were 47.06%, 33.70%, 24.19% and 43.09%, respectively. In addition, studies have also found that both RJP and RJP hydrolysates can reduce the production of 8-OH-2′-dG and the order of its inhibitory ability is RJP-AF ≒ RJP-A > RJP-F > RJP. The inhibition of DNA damage induced by bleomycin-Fe<sup>3+</sup>/ascorbic acid (Asc) with the addition of RJP, RJP-A, RPJ-F and RPJ-AF were 17.16%, 30.88%, 25.00% and 37.25%, respectively. The results of LDL oxidation inhibition showed that RJP-AF (1 mg/mL) not only had the most effective inhibitory Cu<sup>2+</sup>-induced LDL oxidation to produce a thiobarbituric acid reactive substance (TBARS) but also extended the lag time of conjugated diene formation to 300 min, which was 3.3 times that of the control group.https://www.mdpi.com/2076-3921/10/4/580royal jellyprotein hydrolysateDNA oxidative damageLDL oxidationconjugated dieneFenton reaction |
| spellingShingle | Shu-Hua Chiang Kia-Min Yang Shiann-Cherng Sheu Chih-Wei Chen The Bioactive Compound Contents and Potential Protective Effects of Royal Jelly Protein Hydrolysates against DNA Oxidative Damage and LDL Oxidation Antioxidants royal jelly protein hydrolysate DNA oxidative damage LDL oxidation conjugated diene Fenton reaction |
| title | The Bioactive Compound Contents and Potential Protective Effects of Royal Jelly Protein Hydrolysates against DNA Oxidative Damage and LDL Oxidation |
| title_full | The Bioactive Compound Contents and Potential Protective Effects of Royal Jelly Protein Hydrolysates against DNA Oxidative Damage and LDL Oxidation |
| title_fullStr | The Bioactive Compound Contents and Potential Protective Effects of Royal Jelly Protein Hydrolysates against DNA Oxidative Damage and LDL Oxidation |
| title_full_unstemmed | The Bioactive Compound Contents and Potential Protective Effects of Royal Jelly Protein Hydrolysates against DNA Oxidative Damage and LDL Oxidation |
| title_short | The Bioactive Compound Contents and Potential Protective Effects of Royal Jelly Protein Hydrolysates against DNA Oxidative Damage and LDL Oxidation |
| title_sort | bioactive compound contents and potential protective effects of royal jelly protein hydrolysates against dna oxidative damage and ldl oxidation |
| topic | royal jelly protein hydrolysate DNA oxidative damage LDL oxidation conjugated diene Fenton reaction |
| url | https://www.mdpi.com/2076-3921/10/4/580 |
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