Molecular survey of aminoglycoside-resistant Acinetobacter baumannii isolated from tertiary hospitals in Qazvin, Iran
Aminoglycoside-modifying enzymes (AMEs) and 16S rRNA methylases (16S RMTase) are two main resistance mechanisms against aminoglycosides. This study aimed to evaluate the frequency of AMEs and 16S rRNA methylase genes among aminoglycoside non-susceptible Acinetobacter baumannii isolates and to assess...
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Elsevier
2021-07-01
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| Series: | New Microbes and New Infections |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2052297521000470 |
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| author | P. Rashvand A. Peymani M. Mohammadi A.A. Karami R. Samimi S. Hajian D. Porasgari N. Habibollah-Pourzereshki |
| author_facet | P. Rashvand A. Peymani M. Mohammadi A.A. Karami R. Samimi S. Hajian D. Porasgari N. Habibollah-Pourzereshki |
| author_sort | P. Rashvand |
| collection | DOAJ |
| description | Aminoglycoside-modifying enzymes (AMEs) and 16S rRNA methylases (16S RMTase) are two main resistance mechanisms against aminoglycosides. This study aimed to evaluate the frequency of AMEs and 16S rRNA methylase genes among aminoglycoside non-susceptible Acinetobacter baumannii isolates and to assess their clonal relationship using repetitive extragenic palindromic-PCR (rep-PCR). In this cross-sectional study, a total of 192 A. baumannii isolates were collected from the patients hospitalized in Qazvin, Iran (January 2016 to January 2018). Identification of isolates was performed by standard laboratory methods and API 20E strips. Antimicrobial susceptibility was determined by Kirby–Bauer method followed by examination of the genes encoding the AMEs and 16S RMTase by PCR and sequencing methods. The clonal relationship of isolates was carried out by rep-PCR. In total, 98.4% of isolates were non-susceptible to aminoglycosides, 98.4%, 97.9% and 83.9% of isolates were found to be non-susceptible against gentamicin, tobramycin and amikacin, respectively. The frequencies of aph(3′)-VI, aac(6′)-Ib, aac(3)-II, aph(3′)-Ia and armA genes were 59.3%, 39.2%, 39.2%, 31.7% and 69.8%, respectively, either alone or in combination. Rep-PCR results showed that the aminoglycoside non-susceptible isolates belonged to three distinct clones: A (79.4%), B (17.5%) and C (3.2%). The findings of this study showed a high frequency for AMEs with the emergence of armA genes among the aminoglycoside non-susceptible A. baumannii isolates. Rational administration of aminoglycosides as well as using an appropriate infection control policy may reduce the presence of resistance to antibiotics in medical centres. |
| first_indexed | 2024-12-13T19:26:36Z |
| format | Article |
| id | doaj.art-936706b92895403ca1f731ce5affb9da |
| institution | Directory Open Access Journal |
| issn | 2052-2975 |
| language | English |
| last_indexed | 2024-12-13T19:26:36Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
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| series | New Microbes and New Infections |
| spelling | doaj.art-936706b92895403ca1f731ce5affb9da2022-12-21T23:34:02ZengElsevierNew Microbes and New Infections2052-29752021-07-0142100883Molecular survey of aminoglycoside-resistant Acinetobacter baumannii isolated from tertiary hospitals in Qazvin, IranP. Rashvand0A. Peymani1M. Mohammadi2A.A. Karami3R. Samimi4S. Hajian5D. Porasgari6N. Habibollah-Pourzereshki7Student Research Committee, Qazvin University of Medical Sciences, Qazvin, IranMedical Microbiology Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran; Corresponding author: A. Peymani, Medical Microbiology Research Centre, Qazvin University of Medical Sciences, Minoodar, Velayat Hospital, Qazvin, Iran.Student Research Committee, Qazvin University of Medical Sciences, Qazvin, IranDepartment of Urology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, IranMedical Microbiology Research Centre, Qazvin University of Medical Sciences, Qazvin, IranDepartment of Nephrology, Velayat Hospital, Qazvin University of Medical Sciences, Qazvin, IranMedical Microbiology Research Centre, Qazvin University of Medical Sciences, Qazvin, IranMedical Microbiology Research Centre, Qazvin University of Medical Sciences, Qazvin, IranAminoglycoside-modifying enzymes (AMEs) and 16S rRNA methylases (16S RMTase) are two main resistance mechanisms against aminoglycosides. This study aimed to evaluate the frequency of AMEs and 16S rRNA methylase genes among aminoglycoside non-susceptible Acinetobacter baumannii isolates and to assess their clonal relationship using repetitive extragenic palindromic-PCR (rep-PCR). In this cross-sectional study, a total of 192 A. baumannii isolates were collected from the patients hospitalized in Qazvin, Iran (January 2016 to January 2018). Identification of isolates was performed by standard laboratory methods and API 20E strips. Antimicrobial susceptibility was determined by Kirby–Bauer method followed by examination of the genes encoding the AMEs and 16S RMTase by PCR and sequencing methods. The clonal relationship of isolates was carried out by rep-PCR. In total, 98.4% of isolates were non-susceptible to aminoglycosides, 98.4%, 97.9% and 83.9% of isolates were found to be non-susceptible against gentamicin, tobramycin and amikacin, respectively. The frequencies of aph(3′)-VI, aac(6′)-Ib, aac(3)-II, aph(3′)-Ia and armA genes were 59.3%, 39.2%, 39.2%, 31.7% and 69.8%, respectively, either alone or in combination. Rep-PCR results showed that the aminoglycoside non-susceptible isolates belonged to three distinct clones: A (79.4%), B (17.5%) and C (3.2%). The findings of this study showed a high frequency for AMEs with the emergence of armA genes among the aminoglycoside non-susceptible A. baumannii isolates. Rational administration of aminoglycosides as well as using an appropriate infection control policy may reduce the presence of resistance to antibiotics in medical centres.http://www.sciencedirect.com/science/article/pii/S205229752100047016S rRNA methylasesAcinetobacter baumanniiaminoglycoside-modifying enzymesrepetitive extragenic palindromic-PCR |
| spellingShingle | P. Rashvand A. Peymani M. Mohammadi A.A. Karami R. Samimi S. Hajian D. Porasgari N. Habibollah-Pourzereshki Molecular survey of aminoglycoside-resistant Acinetobacter baumannii isolated from tertiary hospitals in Qazvin, Iran New Microbes and New Infections 16S rRNA methylases Acinetobacter baumannii aminoglycoside-modifying enzymes repetitive extragenic palindromic-PCR |
| title | Molecular survey of aminoglycoside-resistant Acinetobacter baumannii isolated from tertiary hospitals in Qazvin, Iran |
| title_full | Molecular survey of aminoglycoside-resistant Acinetobacter baumannii isolated from tertiary hospitals in Qazvin, Iran |
| title_fullStr | Molecular survey of aminoglycoside-resistant Acinetobacter baumannii isolated from tertiary hospitals in Qazvin, Iran |
| title_full_unstemmed | Molecular survey of aminoglycoside-resistant Acinetobacter baumannii isolated from tertiary hospitals in Qazvin, Iran |
| title_short | Molecular survey of aminoglycoside-resistant Acinetobacter baumannii isolated from tertiary hospitals in Qazvin, Iran |
| title_sort | molecular survey of aminoglycoside resistant acinetobacter baumannii isolated from tertiary hospitals in qazvin iran |
| topic | 16S rRNA methylases Acinetobacter baumannii aminoglycoside-modifying enzymes repetitive extragenic palindromic-PCR |
| url | http://www.sciencedirect.com/science/article/pii/S2052297521000470 |
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