Antibacterial efficacy of Trachyspermum ammi Oil against Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans: An in vitro study
Background: Ajwain (Trachyspermum ammi) oil is used widely because of its nutritional value, antioxidant and antibacterial activity against various microorganisms, but there are no studies that relate the antibacterial activity of Ajwain oil against periodontal pathogens. Aim: To evaluate and compar...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Journal of Indian Association of Public Health Dentistry |
| Subjects: | |
| Online Access: | http://www.jiaphd.org/article.asp?issn=2319-5932;year=2022;volume=20;issue=4;spage=398;epage=402;aulast=Yavagal |
| _version_ | 1797954874412892160 |
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| author | Puja C Yavagal Shraddha Shivakumar Rajeshwar |
| author_facet | Puja C Yavagal Shraddha Shivakumar Rajeshwar |
| author_sort | Puja C Yavagal |
| collection | DOAJ |
| description | Background: Ajwain (Trachyspermum ammi) oil is used widely because of its nutritional value, antioxidant and antibacterial activity against various microorganisms, but there are no studies that relate the antibacterial activity of Ajwain oil against periodontal pathogens. Aim: To evaluate and compare the minimum inhibitory concentration (MIC) and zone of inhibition of T. ammi (Ajwain seed) oil and 0.2% chlorhexidine gluconate against Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans through serial tube dilution and disc diffusion methods. Materials and Methods: An in vitro study was conducted. Ajwain oil (100%) and chlorhexidine solution (0.2%) were purchased. Blood agar media with hemin, Vitamin K and Kanamycin, crystal violet erythromycin blood agar, and thioglycolate broth with 1% horse serum were used to culture P. gingivalis, F. nucleatum, and A. actinomycetemcomitans, respectively. The MIC and minimum bactericidal concentration of test agents were assessed using the serial dilution method, and the zone of inhibition was assessed by the Agar disc diffusion method. Results: Ajwain oil had the ability to exert antibacterial activity against P. gingivalis, F. nucleatum, and A. actinomycetemcomitans at a MIC of 0.4, 0.4, and 0.8 μg/ml, respectively. Ajwain oil exerted bactericidal effect against P gingivalis, F. nucleatum, and A. actinomycetemcomitans at a minimum concentration of 0.4, 0.4, and 6.25 μg/ml, respectively, and bacteriostatic effect at a minimum concentration of 0.2, 0.2, and 3.12 μg/ml, respectively. At 50% μg/ml concentration, Ajwain oil exhibited a higher zone of inhibition compared to chlorhexidine against P. gingivalis, F. nucleatum, and A. actinomycetemcomitans. Conclusion: Ajwain oil demonstrated antibacterial effect against periodontal pathogens in vitro. |
| first_indexed | 2024-04-10T23:24:20Z |
| format | Article |
| id | doaj.art-93692e456d644b4ab2adee15342c69a6 |
| institution | Directory Open Access Journal |
| issn | 2319-5932 2350-0484 |
| language | English |
| last_indexed | 2024-04-10T23:24:20Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Indian Association of Public Health Dentistry |
| spelling | doaj.art-93692e456d644b4ab2adee15342c69a62023-01-12T12:42:42ZengWolters Kluwer Medknow PublicationsJournal of Indian Association of Public Health Dentistry2319-59322350-04842022-01-0120439840210.4103/jiaphd.jiaphd_170_21Antibacterial efficacy of Trachyspermum ammi Oil against Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans: An in vitro studyPuja C YavagalShraddha Shivakumar RajeshwarBackground: Ajwain (Trachyspermum ammi) oil is used widely because of its nutritional value, antioxidant and antibacterial activity against various microorganisms, but there are no studies that relate the antibacterial activity of Ajwain oil against periodontal pathogens. Aim: To evaluate and compare the minimum inhibitory concentration (MIC) and zone of inhibition of T. ammi (Ajwain seed) oil and 0.2% chlorhexidine gluconate against Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans through serial tube dilution and disc diffusion methods. Materials and Methods: An in vitro study was conducted. Ajwain oil (100%) and chlorhexidine solution (0.2%) were purchased. Blood agar media with hemin, Vitamin K and Kanamycin, crystal violet erythromycin blood agar, and thioglycolate broth with 1% horse serum were used to culture P. gingivalis, F. nucleatum, and A. actinomycetemcomitans, respectively. The MIC and minimum bactericidal concentration of test agents were assessed using the serial dilution method, and the zone of inhibition was assessed by the Agar disc diffusion method. Results: Ajwain oil had the ability to exert antibacterial activity against P. gingivalis, F. nucleatum, and A. actinomycetemcomitans at a MIC of 0.4, 0.4, and 0.8 μg/ml, respectively. Ajwain oil exerted bactericidal effect against P gingivalis, F. nucleatum, and A. actinomycetemcomitans at a minimum concentration of 0.4, 0.4, and 6.25 μg/ml, respectively, and bacteriostatic effect at a minimum concentration of 0.2, 0.2, and 3.12 μg/ml, respectively. At 50% μg/ml concentration, Ajwain oil exhibited a higher zone of inhibition compared to chlorhexidine against P. gingivalis, F. nucleatum, and A. actinomycetemcomitans. Conclusion: Ajwain oil demonstrated antibacterial effect against periodontal pathogens in vitro.http://www.jiaphd.org/article.asp?issn=2319-5932;year=2022;volume=20;issue=4;spage=398;epage=402;aulast=Yavagalaggregatibacter actinomycetemcomitansajwain oilchlorhexidinefusobacterium nucleatumporphyromonas gingivalis |
| spellingShingle | Puja C Yavagal Shraddha Shivakumar Rajeshwar Antibacterial efficacy of Trachyspermum ammi Oil against Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans: An in vitro study Journal of Indian Association of Public Health Dentistry aggregatibacter actinomycetemcomitans ajwain oil chlorhexidine fusobacterium nucleatum porphyromonas gingivalis |
| title | Antibacterial efficacy of Trachyspermum ammi Oil against Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans: An in vitro study |
| title_full | Antibacterial efficacy of Trachyspermum ammi Oil against Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans: An in vitro study |
| title_fullStr | Antibacterial efficacy of Trachyspermum ammi Oil against Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans: An in vitro study |
| title_full_unstemmed | Antibacterial efficacy of Trachyspermum ammi Oil against Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans: An in vitro study |
| title_short | Antibacterial efficacy of Trachyspermum ammi Oil against Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans: An in vitro study |
| title_sort | antibacterial efficacy of trachyspermum ammi oil against porphyromonas gingivalis fusobacterium nucleatum and aggregatibacter actinomycetemcomitans an in vitro study |
| topic | aggregatibacter actinomycetemcomitans ajwain oil chlorhexidine fusobacterium nucleatum porphyromonas gingivalis |
| url | http://www.jiaphd.org/article.asp?issn=2319-5932;year=2022;volume=20;issue=4;spage=398;epage=402;aulast=Yavagal |
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