Increased seizure susceptibility in mice 30 days after fluid percussion injury.
Traumatic brain injury (TBI) has been reported to increase seizure susceptibility and also contribute to the development of epilepsy. However, the mechanistic basis of the development of increased seizure susceptibility and epilepsy is not clear. Though there is substantial work done using rats, dat...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-03-01
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| Series: | Frontiers in Neurology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fneur.2013.00028/full |
| _version_ | 1818615017467019264 |
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| author | Sanjib eMukherjee Sanjib eMukherjee Suzanne eZeitouni Suzanne eZeitouni Clarissa Fantin Cavarsan Clarissa Fantin Cavarsan Lee A Shapiro Lee A Shapiro Lee A Shapiro |
| author_facet | Sanjib eMukherjee Sanjib eMukherjee Suzanne eZeitouni Suzanne eZeitouni Clarissa Fantin Cavarsan Clarissa Fantin Cavarsan Lee A Shapiro Lee A Shapiro Lee A Shapiro |
| author_sort | Sanjib eMukherjee |
| collection | DOAJ |
| description | Traumatic brain injury (TBI) has been reported to increase seizure susceptibility and also contribute to the development of epilepsy. However, the mechanistic basis of the development of increased seizure susceptibility and epilepsy is not clear. Though there is substantial work done using rats, data are lacking regarding the use of mice in the fluid percussion injury (FPI) model. It is unclear if mice, like rats, will experience increased seizure susceptibility following FPI. The availability of a mouse model of increased seizure susceptibility after FPI would provide a basis for the use of genetically modified mice to study mechanism(s) of the development of post-traumatic epilepsy. Therefore, this study was designed to test the hypothesis that, mice subjected to a FPI develop increased seizure susceptibility to a subconvulsive dose of the chemoconvulsant, pentylenetetrazole (PTZ). Three groups of mice were used: FPI, sham and naïve controls. On day thirty after FPI, mice from the three groups were injected with PTZ. The results showed that FPI mice exhibited an increased severity, frequency and duration of seizures in response to PTZ injection compared with the sham and naïve control groups. Histopathological assessment was used to characterize the injury at one, three, seven and thirty days after FPI. The results show that mice subjected to the FPI had a pronounced lesion and glial response that was centered at the FPI focus and peaked at three days. By thirty days, only minimal evidence of a lesion is observed, although there is evidence of a chronic glial response. These data are the first to demonstrate an early increase in seizure susceptibility following fluid percussion injury in mice. Therefore, future studies can incorporate transgenic mice into this model to further elucidate mechanisms of TBI-induced increases in seizure susceptibility. |
| first_indexed | 2024-12-16T16:27:13Z |
| format | Article |
| id | doaj.art-936ab175e12742d1aab10c0c245dccf3 |
| institution | Directory Open Access Journal |
| issn | 1664-2295 |
| language | English |
| last_indexed | 2024-12-16T16:27:13Z |
| publishDate | 2013-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neurology |
| spelling | doaj.art-936ab175e12742d1aab10c0c245dccf32022-12-21T22:24:43ZengFrontiers Media S.A.Frontiers in Neurology1664-22952013-03-01410.3389/fneur.2013.0002837318Increased seizure susceptibility in mice 30 days after fluid percussion injury.Sanjib eMukherjee0Sanjib eMukherjee1Suzanne eZeitouni2Suzanne eZeitouni3Clarissa Fantin Cavarsan4Clarissa Fantin Cavarsan5Lee A Shapiro6Lee A Shapiro7Lee A Shapiro8Scott & White HospitalCentral Texas Veterans Health Care SystemTexas A&M University System Health Science Center College of MedicineCentral Texas Veterans Health Care SystemScott & White HospitalCentral Texas Veterans Health Care SystemTexas A&M University System Health Science Center College of MedicineScott & White HospitalCentral Texas Veterans Health Care SystemTraumatic brain injury (TBI) has been reported to increase seizure susceptibility and also contribute to the development of epilepsy. However, the mechanistic basis of the development of increased seizure susceptibility and epilepsy is not clear. Though there is substantial work done using rats, data are lacking regarding the use of mice in the fluid percussion injury (FPI) model. It is unclear if mice, like rats, will experience increased seizure susceptibility following FPI. The availability of a mouse model of increased seizure susceptibility after FPI would provide a basis for the use of genetically modified mice to study mechanism(s) of the development of post-traumatic epilepsy. Therefore, this study was designed to test the hypothesis that, mice subjected to a FPI develop increased seizure susceptibility to a subconvulsive dose of the chemoconvulsant, pentylenetetrazole (PTZ). Three groups of mice were used: FPI, sham and naïve controls. On day thirty after FPI, mice from the three groups were injected with PTZ. The results showed that FPI mice exhibited an increased severity, frequency and duration of seizures in response to PTZ injection compared with the sham and naïve control groups. Histopathological assessment was used to characterize the injury at one, three, seven and thirty days after FPI. The results show that mice subjected to the FPI had a pronounced lesion and glial response that was centered at the FPI focus and peaked at three days. By thirty days, only minimal evidence of a lesion is observed, although there is evidence of a chronic glial response. These data are the first to demonstrate an early increase in seizure susceptibility following fluid percussion injury in mice. Therefore, future studies can incorporate transgenic mice into this model to further elucidate mechanisms of TBI-induced increases in seizure susceptibility.http://journal.frontiersin.org/Journal/10.3389/fneur.2013.00028/fullmouse modelsTraumatic brain injury (TBI)post-traumatic epilepsypentylenetetrazole (PTZ)lateral fluid percussion injury |
| spellingShingle | Sanjib eMukherjee Sanjib eMukherjee Suzanne eZeitouni Suzanne eZeitouni Clarissa Fantin Cavarsan Clarissa Fantin Cavarsan Lee A Shapiro Lee A Shapiro Lee A Shapiro Increased seizure susceptibility in mice 30 days after fluid percussion injury. Frontiers in Neurology mouse models Traumatic brain injury (TBI) post-traumatic epilepsy pentylenetetrazole (PTZ) lateral fluid percussion injury |
| title | Increased seizure susceptibility in mice 30 days after fluid percussion injury. |
| title_full | Increased seizure susceptibility in mice 30 days after fluid percussion injury. |
| title_fullStr | Increased seizure susceptibility in mice 30 days after fluid percussion injury. |
| title_full_unstemmed | Increased seizure susceptibility in mice 30 days after fluid percussion injury. |
| title_short | Increased seizure susceptibility in mice 30 days after fluid percussion injury. |
| title_sort | increased seizure susceptibility in mice 30 days after fluid percussion injury |
| topic | mouse models Traumatic brain injury (TBI) post-traumatic epilepsy pentylenetetrazole (PTZ) lateral fluid percussion injury |
| url | http://journal.frontiersin.org/Journal/10.3389/fneur.2013.00028/full |
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