Effect of dithiocyano-methane on hexose monophosphate pathway in the respiratory metabolism of Escherichia coli
Abstract This paper studied the inhibitory effects of dithiocyano-methane (DM) on the glucose decomposition pathway in the respiratory metabolism of Escherichia coli. We investigated the effects of DM on the activities of key enzymes (ATPase and glucose-6-phosphate dehydrogenase, G6PDH), the levels...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2020-11-01
|
| Series: | AMB Express |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13568-020-01142-z |
| _version_ | 1818110463714525184 |
|---|---|
| author | Yanfeng Chen Wenjie Ke Huabin Qin Siwei Chen Limei Qin Ying Yang Hui Yu Yuansheng Tan |
| author_facet | Yanfeng Chen Wenjie Ke Huabin Qin Siwei Chen Limei Qin Ying Yang Hui Yu Yuansheng Tan |
| author_sort | Yanfeng Chen |
| collection | DOAJ |
| description | Abstract This paper studied the inhibitory effects of dithiocyano-methane (DM) on the glucose decomposition pathway in the respiratory metabolism of Escherichia coli. We investigated the effects of DM on the activities of key enzymes (ATPase and glucose-6-phosphate dehydrogenase, G6PDH), the levels of key product (nicotinamide adenosine denucleotide hydro-phosphoric acid, NADPH), and gene expression in the hexose monophosphate pathway (HMP). The results showed that the minimum inhibitory concentration (MIC) and the minimum bactericide concentration (MBC) of DM against the tested strains were 5.86 mg/L and 11.72 mg/L, respectively. Bacteria exposed to DM at MIC demonstrated an increase in bacterial ATPase and G6PDH activities, NADPH levels, and gene expression in the HMP pathway compared to bacteria in the control group, which could be interpreted as a behavioral response to stress introduced by DM. However, DM at a lethal concentration of 10 × MIC affected glucose decomposition by inhibiting mainly the HMP pathway in E. coli. |
| first_indexed | 2024-12-11T02:47:33Z |
| format | Article |
| id | doaj.art-93750a9fc35043bfbacaeaf22b5e743e |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-11T02:47:33Z |
| publishDate | 2020-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-93750a9fc35043bfbacaeaf22b5e743e2022-12-22T01:23:24ZengSpringerOpenAMB Express2191-08552020-11-0110111110.1186/s13568-020-01142-zEffect of dithiocyano-methane on hexose monophosphate pathway in the respiratory metabolism of Escherichia coliYanfeng Chen0Wenjie Ke1Huabin Qin2Siwei Chen3Limei Qin4Ying Yang5Hui Yu6Yuansheng Tan7Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes, School of Life Science and Engineering, Foshan UniversityGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes, School of Life Science and Engineering, Foshan UniversityGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes, School of Life Science and Engineering, Foshan UniversityGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes, School of Life Science and Engineering, Foshan UniversityGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes, School of Life Science and Engineering, Foshan UniversityGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes, School of Life Science and Engineering, Foshan UniversityGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes, School of Life Science and Engineering, Foshan UniversityGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes, School of Life Science and Engineering, Foshan UniversityAbstract This paper studied the inhibitory effects of dithiocyano-methane (DM) on the glucose decomposition pathway in the respiratory metabolism of Escherichia coli. We investigated the effects of DM on the activities of key enzymes (ATPase and glucose-6-phosphate dehydrogenase, G6PDH), the levels of key product (nicotinamide adenosine denucleotide hydro-phosphoric acid, NADPH), and gene expression in the hexose monophosphate pathway (HMP). The results showed that the minimum inhibitory concentration (MIC) and the minimum bactericide concentration (MBC) of DM against the tested strains were 5.86 mg/L and 11.72 mg/L, respectively. Bacteria exposed to DM at MIC demonstrated an increase in bacterial ATPase and G6PDH activities, NADPH levels, and gene expression in the HMP pathway compared to bacteria in the control group, which could be interpreted as a behavioral response to stress introduced by DM. However, DM at a lethal concentration of 10 × MIC affected glucose decomposition by inhibiting mainly the HMP pathway in E. coli.http://link.springer.com/article/10.1186/s13568-020-01142-zDithiocyano-methaneHexose monophosphate pathwayRespiratory metabolismEscherichia coli |
| spellingShingle | Yanfeng Chen Wenjie Ke Huabin Qin Siwei Chen Limei Qin Ying Yang Hui Yu Yuansheng Tan Effect of dithiocyano-methane on hexose monophosphate pathway in the respiratory metabolism of Escherichia coli AMB Express Dithiocyano-methane Hexose monophosphate pathway Respiratory metabolism Escherichia coli |
| title | Effect of dithiocyano-methane on hexose monophosphate pathway in the respiratory metabolism of Escherichia coli |
| title_full | Effect of dithiocyano-methane on hexose monophosphate pathway in the respiratory metabolism of Escherichia coli |
| title_fullStr | Effect of dithiocyano-methane on hexose monophosphate pathway in the respiratory metabolism of Escherichia coli |
| title_full_unstemmed | Effect of dithiocyano-methane on hexose monophosphate pathway in the respiratory metabolism of Escherichia coli |
| title_short | Effect of dithiocyano-methane on hexose monophosphate pathway in the respiratory metabolism of Escherichia coli |
| title_sort | effect of dithiocyano methane on hexose monophosphate pathway in the respiratory metabolism of escherichia coli |
| topic | Dithiocyano-methane Hexose monophosphate pathway Respiratory metabolism Escherichia coli |
| url | http://link.springer.com/article/10.1186/s13568-020-01142-z |
| work_keys_str_mv | AT yanfengchen effectofdithiocyanomethaneonhexosemonophosphatepathwayintherespiratorymetabolismofescherichiacoli AT wenjieke effectofdithiocyanomethaneonhexosemonophosphatepathwayintherespiratorymetabolismofescherichiacoli AT huabinqin effectofdithiocyanomethaneonhexosemonophosphatepathwayintherespiratorymetabolismofescherichiacoli AT siweichen effectofdithiocyanomethaneonhexosemonophosphatepathwayintherespiratorymetabolismofescherichiacoli AT limeiqin effectofdithiocyanomethaneonhexosemonophosphatepathwayintherespiratorymetabolismofescherichiacoli AT yingyang effectofdithiocyanomethaneonhexosemonophosphatepathwayintherespiratorymetabolismofescherichiacoli AT huiyu effectofdithiocyanomethaneonhexosemonophosphatepathwayintherespiratorymetabolismofescherichiacoli AT yuanshengtan effectofdithiocyanomethaneonhexosemonophosphatepathwayintherespiratorymetabolismofescherichiacoli |