The multifaceted roles of Arbuscular Mycorrhizal Fungi in peanut responses to salt, drought, and cold stress
Abstract Background Arbuscular Mycorrhizal Fungi (AMF) are beneficial microorganisms in soil-plant interactions; however, the underlying mechanisms regarding their roles in legumes environmental stress remain elusive. Present trials were undertaken to study the effect of AMF on the ameliorating of s...
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| Format: | Article |
| Language: | English |
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BMC
2023-01-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-023-04053-w |
| _version_ | 1797946029108101120 |
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| author | Yuexu Liu Jinhao Lu Li Cui Zhaohui Tang Dunwei Ci Xiaoxia Zou Xiaojun Zhang Xiaona Yu Yuefu Wang Tong Si |
| author_facet | Yuexu Liu Jinhao Lu Li Cui Zhaohui Tang Dunwei Ci Xiaoxia Zou Xiaojun Zhang Xiaona Yu Yuefu Wang Tong Si |
| author_sort | Yuexu Liu |
| collection | DOAJ |
| description | Abstract Background Arbuscular Mycorrhizal Fungi (AMF) are beneficial microorganisms in soil-plant interactions; however, the underlying mechanisms regarding their roles in legumes environmental stress remain elusive. Present trials were undertaken to study the effect of AMF on the ameliorating of salt, drought, and cold stress in peanut (Arachis hypogaea L.) plants. A new product of AMF combined with Rhizophagus irregularis SA, Rhizophagus clarus BEG142, Glomus lamellosum ON393, and Funneliformis mosseae BEG95 (1: 1: 1: 1, w/w/w/w) was inoculated with peanut and the physiological and metabolomic responses of the AMF-inoculated and non-inoculated peanut plants to salt, drought, and cold stress were comprehensively characterized, respectively. Results AMF-inoculated plants exhibited higher plant growth, leaf relative water content (RWC), net photosynthetic rate, maximal photochemical efficiency of photosystem II (PSII) (Fv/Fm), activities of antioxidant enzymes, and K+: Na+ ratio while lower leaf relative electrolyte conductivity (REC), concentration of malondialdehyde (MDA), and the accumulation of reactive oxygen species (ROS) under stressful conditions. Moreover, the structures of chloroplast thylakoids and mitochondria in AMF-inoculated plants were less damaged by these stresses. Non-targeted metabolomics indicated that AMF altered numerous pathways associated with organic acids and amino acid metabolisms in peanut roots under both normal-growth and stressful conditions, which were further improved by the osmolytes accumulation data. Conclusion This study provides a promising AMF product and demonstrates that this AMF combination could enhance peanut salt, drought, and cold stress tolerance through improving plant growth, protecting photosystem, enhancing antioxidant system, and regulating osmotic adjustment. |
| first_indexed | 2024-04-10T21:04:26Z |
| format | Article |
| id | doaj.art-93770fd97abc4930a78ba269d1797a94 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-10T21:04:26Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-93770fd97abc4930a78ba269d1797a942023-01-22T12:08:47ZengBMCBMC Plant Biology1471-22292023-01-0123111910.1186/s12870-023-04053-wThe multifaceted roles of Arbuscular Mycorrhizal Fungi in peanut responses to salt, drought, and cold stressYuexu Liu0Jinhao Lu1Li Cui2Zhaohui Tang3Dunwei Ci4Xiaoxia Zou5Xiaojun Zhang6Xiaona Yu7Yuefu Wang8Tong Si9Shandong Provincial Key Laboratory of Dryland Farming Technology,College of Agronomy, Qingdao Agricultural UniversityShandong Provincial Key Laboratory of Dryland Farming Technology,College of Agronomy, Qingdao Agricultural UniversityInstitute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences (SAAS)Institute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences (SAAS)Shandong Peanut Research InstituteShandong Provincial Key Laboratory of Dryland Farming Technology,College of Agronomy, Qingdao Agricultural UniversityShandong Provincial Key Laboratory of Dryland Farming Technology,College of Agronomy, Qingdao Agricultural UniversityShandong Provincial Key Laboratory of Dryland Farming Technology,College of Agronomy, Qingdao Agricultural UniversityShandong Provincial Key Laboratory of Dryland Farming Technology,College of Agronomy, Qingdao Agricultural UniversityShandong Provincial Key Laboratory of Dryland Farming Technology,College of Agronomy, Qingdao Agricultural UniversityAbstract Background Arbuscular Mycorrhizal Fungi (AMF) are beneficial microorganisms in soil-plant interactions; however, the underlying mechanisms regarding their roles in legumes environmental stress remain elusive. Present trials were undertaken to study the effect of AMF on the ameliorating of salt, drought, and cold stress in peanut (Arachis hypogaea L.) plants. A new product of AMF combined with Rhizophagus irregularis SA, Rhizophagus clarus BEG142, Glomus lamellosum ON393, and Funneliformis mosseae BEG95 (1: 1: 1: 1, w/w/w/w) was inoculated with peanut and the physiological and metabolomic responses of the AMF-inoculated and non-inoculated peanut plants to salt, drought, and cold stress were comprehensively characterized, respectively. Results AMF-inoculated plants exhibited higher plant growth, leaf relative water content (RWC), net photosynthetic rate, maximal photochemical efficiency of photosystem II (PSII) (Fv/Fm), activities of antioxidant enzymes, and K+: Na+ ratio while lower leaf relative electrolyte conductivity (REC), concentration of malondialdehyde (MDA), and the accumulation of reactive oxygen species (ROS) under stressful conditions. Moreover, the structures of chloroplast thylakoids and mitochondria in AMF-inoculated plants were less damaged by these stresses. Non-targeted metabolomics indicated that AMF altered numerous pathways associated with organic acids and amino acid metabolisms in peanut roots under both normal-growth and stressful conditions, which were further improved by the osmolytes accumulation data. Conclusion This study provides a promising AMF product and demonstrates that this AMF combination could enhance peanut salt, drought, and cold stress tolerance through improving plant growth, protecting photosystem, enhancing antioxidant system, and regulating osmotic adjustment.https://doi.org/10.1186/s12870-023-04053-wAMFLegumesEnvironmental stressPlant physiologyMetabolic pathway |
| spellingShingle | Yuexu Liu Jinhao Lu Li Cui Zhaohui Tang Dunwei Ci Xiaoxia Zou Xiaojun Zhang Xiaona Yu Yuefu Wang Tong Si The multifaceted roles of Arbuscular Mycorrhizal Fungi in peanut responses to salt, drought, and cold stress BMC Plant Biology AMF Legumes Environmental stress Plant physiology Metabolic pathway |
| title | The multifaceted roles of Arbuscular Mycorrhizal Fungi in peanut responses to salt, drought, and cold stress |
| title_full | The multifaceted roles of Arbuscular Mycorrhizal Fungi in peanut responses to salt, drought, and cold stress |
| title_fullStr | The multifaceted roles of Arbuscular Mycorrhizal Fungi in peanut responses to salt, drought, and cold stress |
| title_full_unstemmed | The multifaceted roles of Arbuscular Mycorrhizal Fungi in peanut responses to salt, drought, and cold stress |
| title_short | The multifaceted roles of Arbuscular Mycorrhizal Fungi in peanut responses to salt, drought, and cold stress |
| title_sort | multifaceted roles of arbuscular mycorrhizal fungi in peanut responses to salt drought and cold stress |
| topic | AMF Legumes Environmental stress Plant physiology Metabolic pathway |
| url | https://doi.org/10.1186/s12870-023-04053-w |
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