Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry
Abstract Cryopreservation of ovarian tissue followed by transplantation represents a strategy to restore ovarian function and fertility. Stress from cryopreservation-thawing processes can lead to alterations and/or damage to mitochondrial structure and functionality. High resolution respirometry and...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-11-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-01082-z |
| _version_ | 1818894454407299072 |
|---|---|
| author | Aline Q. Rodrigues Victor L. Picolo Jair T. Goulart Isabella M. G. Silva Rayane B. Ribeiro Beatriz A. Aguiar Yasmin B. Ferreira Daniela M. Oliveira Carolina M. Lucci Andreza F. de Bem Fernanda Paulini |
| author_facet | Aline Q. Rodrigues Victor L. Picolo Jair T. Goulart Isabella M. G. Silva Rayane B. Ribeiro Beatriz A. Aguiar Yasmin B. Ferreira Daniela M. Oliveira Carolina M. Lucci Andreza F. de Bem Fernanda Paulini |
| author_sort | Aline Q. Rodrigues |
| collection | DOAJ |
| description | Abstract Cryopreservation of ovarian tissue followed by transplantation represents a strategy to restore ovarian function and fertility. Stress from cryopreservation-thawing processes can lead to alterations and/or damage to mitochondrial structure and functionality. High resolution respirometry and histological analysis were used to evaluate the effect of cryopreservation and transplantation on ovarian tissue. Four different conditions were performed: Fresh non-transplanted tissue, Fresh transplanted tissue, Cryopreserved non-transplanted tissue and Cryopreserved transplanted tissue. All groups were able to respond to the substrates-uncoupler-inhibitor protocol. We found a dramatic decrease in general oxygen consumption in hemi-ovaries submitted to cryopreservation and/or transplantation. The effect of cryopreservation on mitochondrial metabolism was less intense than effect of transplantation, since the transplantation affected all of the mitochondrial states. A total of 2644 follicles were analyzed. Of these, 2198 were classified as morphologically normal. The percentage of morphologically normal follicles was significantly lower in the Cryopreserved transplanted group when compared to the Cryopreserved non-transplanted group and the Fresh transplanted group (p-value < 0.05). Despite decreased follicular viability and mitochondrial activity, the cryopreservation followed by transplantation of ovarian tissue proved feasible for attempts to restore ovarian function. |
| first_indexed | 2024-12-19T18:28:45Z |
| format | Article |
| id | doaj.art-937c0480c522419c99b23c33d022ca12 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T18:28:45Z |
| publishDate | 2021-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-937c0480c522419c99b23c33d022ca122022-12-21T20:10:47ZengNature PortfolioScientific Reports2045-23222021-11-0111111010.1038/s41598-021-01082-zMetabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometryAline Q. Rodrigues0Victor L. Picolo1Jair T. Goulart2Isabella M. G. Silva3Rayane B. Ribeiro4Beatriz A. Aguiar5Yasmin B. Ferreira6Daniela M. Oliveira7Carolina M. Lucci8Andreza F. de Bem9Fernanda Paulini10Department of Physiological Sciences, Institute of Biological Sciences, University of BrasíliaDepartment of Physiological Sciences, Institute of Biological Sciences, University of BrasíliaDepartment of Physiological Sciences, Institute of Biological Sciences, University of BrasíliaDepartment of Physiological Sciences, Institute of Biological Sciences, University of BrasíliaDepartment of Physiological Sciences, Institute of Biological Sciences, University of BrasíliaDepartment of Physiological Sciences, Institute of Biological Sciences, University of BrasíliaDepartment of Physiological Sciences, Institute of Biological Sciences, University of BrasíliaDepartment of Genetics and Morphology, Institute of Biological Sciences, University of BrasíliaDepartment of Physiological Sciences, Institute of Biological Sciences, University of BrasíliaDepartment of Physiological Sciences, Institute of Biological Sciences, University of BrasíliaDepartment of Physiological Sciences, Institute of Biological Sciences, University of BrasíliaAbstract Cryopreservation of ovarian tissue followed by transplantation represents a strategy to restore ovarian function and fertility. Stress from cryopreservation-thawing processes can lead to alterations and/or damage to mitochondrial structure and functionality. High resolution respirometry and histological analysis were used to evaluate the effect of cryopreservation and transplantation on ovarian tissue. Four different conditions were performed: Fresh non-transplanted tissue, Fresh transplanted tissue, Cryopreserved non-transplanted tissue and Cryopreserved transplanted tissue. All groups were able to respond to the substrates-uncoupler-inhibitor protocol. We found a dramatic decrease in general oxygen consumption in hemi-ovaries submitted to cryopreservation and/or transplantation. The effect of cryopreservation on mitochondrial metabolism was less intense than effect of transplantation, since the transplantation affected all of the mitochondrial states. A total of 2644 follicles were analyzed. Of these, 2198 were classified as morphologically normal. The percentage of morphologically normal follicles was significantly lower in the Cryopreserved transplanted group when compared to the Cryopreserved non-transplanted group and the Fresh transplanted group (p-value < 0.05). Despite decreased follicular viability and mitochondrial activity, the cryopreservation followed by transplantation of ovarian tissue proved feasible for attempts to restore ovarian function.https://doi.org/10.1038/s41598-021-01082-z |
| spellingShingle | Aline Q. Rodrigues Victor L. Picolo Jair T. Goulart Isabella M. G. Silva Rayane B. Ribeiro Beatriz A. Aguiar Yasmin B. Ferreira Daniela M. Oliveira Carolina M. Lucci Andreza F. de Bem Fernanda Paulini Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry Scientific Reports |
| title | Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry |
| title_full | Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry |
| title_fullStr | Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry |
| title_full_unstemmed | Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry |
| title_short | Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry |
| title_sort | metabolic activity in cryopreserved and grafted ovarian tissue using high resolution respirometry |
| url | https://doi.org/10.1038/s41598-021-01082-z |
| work_keys_str_mv | AT alineqrodrigues metabolicactivityincryopreservedandgraftedovariantissueusinghighresolutionrespirometry AT victorlpicolo metabolicactivityincryopreservedandgraftedovariantissueusinghighresolutionrespirometry AT jairtgoulart metabolicactivityincryopreservedandgraftedovariantissueusinghighresolutionrespirometry AT isabellamgsilva metabolicactivityincryopreservedandgraftedovariantissueusinghighresolutionrespirometry AT rayanebribeiro metabolicactivityincryopreservedandgraftedovariantissueusinghighresolutionrespirometry AT beatrizaaguiar metabolicactivityincryopreservedandgraftedovariantissueusinghighresolutionrespirometry AT yasminbferreira metabolicactivityincryopreservedandgraftedovariantissueusinghighresolutionrespirometry AT danielamoliveira metabolicactivityincryopreservedandgraftedovariantissueusinghighresolutionrespirometry AT carolinamlucci metabolicactivityincryopreservedandgraftedovariantissueusinghighresolutionrespirometry AT andrezafdebem metabolicactivityincryopreservedandgraftedovariantissueusinghighresolutionrespirometry AT fernandapaulini metabolicactivityincryopreservedandgraftedovariantissueusinghighresolutionrespirometry |