Characterization of mitochondrial iron uptake in HepG2 cells
There is increasing evidence that accumulation of redox-active iron in mitochondria leads to oxidative damage and contributes to various neurodegenerative diseases, such as Friedreich's ataxia and Parkinson's disease. In this work, we examined the existence of regulatory mechanisms for mit...
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| Format: | Article |
| Language: | English |
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BMC
2006-01-01
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100024 |
| _version_ | 1818243910179225600 |
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| author | DORA INÉS MAZARIEGOS Z. IOAV CABANTCHIK BRUCE K CASSELS MARCO TULIO NÚÑEZ |
| author_facet | DORA INÉS MAZARIEGOS Z. IOAV CABANTCHIK BRUCE K CASSELS MARCO TULIO NÚÑEZ |
| author_sort | DORA INÉS MAZARIEGOS |
| collection | DOAJ |
| description | There is increasing evidence that accumulation of redox-active iron in mitochondria leads to oxidative damage and contributes to various neurodegenerative diseases, such as Friedreich's ataxia and Parkinson's disease. In this work, we examined the existence of regulatory mechanisms for mitochondrial iron uptake and storage. To that end, we used rhodamine B- [(1,10-phenanthrolin-5-yl)amino carbonyl ] benzyl ester, a new fluorescent iron-sensitive probe that is targeted specifically to the mitochondrion. We found that extracellular iron was incorporated readily into mitochondria in an apparently saturable process. Moreover, the rate of iron incorporation responded to the Fe status of the cell, an indication that the mitochondrion actively regulates its iron content. |
| first_indexed | 2024-12-12T14:08:38Z |
| format | Article |
| id | doaj.art-937e265f6f264e31a60ed78bea8104fd |
| institution | Directory Open Access Journal |
| issn | 0716-9760 0717-6287 |
| language | English |
| last_indexed | 2024-12-12T14:08:38Z |
| publishDate | 2006-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-937e265f6f264e31a60ed78bea8104fd2022-12-22T00:22:09ZengBMCBiological Research0716-97600717-62872006-01-01391199201Characterization of mitochondrial iron uptake in HepG2 cellsDORA INÉS MAZARIEGOSZ. IOAV CABANTCHIKBRUCE K CASSELSMARCO TULIO NÚÑEZThere is increasing evidence that accumulation of redox-active iron in mitochondria leads to oxidative damage and contributes to various neurodegenerative diseases, such as Friedreich's ataxia and Parkinson's disease. In this work, we examined the existence of regulatory mechanisms for mitochondrial iron uptake and storage. To that end, we used rhodamine B- [(1,10-phenanthrolin-5-yl)amino carbonyl ] benzyl ester, a new fluorescent iron-sensitive probe that is targeted specifically to the mitochondrion. We found that extracellular iron was incorporated readily into mitochondria in an apparently saturable process. Moreover, the rate of iron incorporation responded to the Fe status of the cell, an indication that the mitochondrion actively regulates its iron content.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100024mitochondriairon homeostasisRPAHepG2 cells |
| spellingShingle | DORA INÉS MAZARIEGOS Z. IOAV CABANTCHIK BRUCE K CASSELS MARCO TULIO NÚÑEZ Characterization of mitochondrial iron uptake in HepG2 cells Biological Research mitochondria iron homeostasis RPA HepG2 cells |
| title | Characterization of mitochondrial iron uptake in HepG2 cells |
| title_full | Characterization of mitochondrial iron uptake in HepG2 cells |
| title_fullStr | Characterization of mitochondrial iron uptake in HepG2 cells |
| title_full_unstemmed | Characterization of mitochondrial iron uptake in HepG2 cells |
| title_short | Characterization of mitochondrial iron uptake in HepG2 cells |
| title_sort | characterization of mitochondrial iron uptake in hepg2 cells |
| topic | mitochondria iron homeostasis RPA HepG2 cells |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100024 |
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