Comparison of histopathological analysis, culture and polymerase chain reaction assays to detect mucormycosis in biopsy and blood specimens
Background and Objectives: The aim of this study was to compare direct microscopic examination with culture and PCR for the diagnosis of Mucorales infection in blood and tissue specimens. Material and Methods: Blood samples and tissue specimens were obtained from 28 patients (total 58 samples) with...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2013-12-01
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| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/488 |
| _version_ | 1818878277679316992 |
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| author | Parisa Badiee Amir Arastefar Hadis Jafarian |
| author_facet | Parisa Badiee Amir Arastefar Hadis Jafarian |
| author_sort | Parisa Badiee |
| collection | DOAJ |
| description | Background and Objectives: The aim of this study was to compare direct microscopic examination with culture and PCR for the diagnosis of Mucorales infection in blood and tissue specimens.
Material and Methods: Blood samples and tissue specimens were obtained from 28 patients (total 58 samples) with suspected invasive fungal infection and cultured on proper media. Direct smear of tissue samples was done with potassium hydroxide, hematoxylin and eosin, and methenamine silver staining. DNA extracted from blood and tissue specimens were used for semi-nested PCR targeting 18S rDNA of Mucorales species.
Results: Mucormycosis was documented in 7/28 (25%) of tissue specimens with positive findings by direct smear, of which PCR and culture were positive in 6 (86%) and 5 (70%) specimens, respectively. The etiologic agents were Mucor spp. and Rhizopus spp. However, culture and PCR results for all blood specimens were negative.
Conclusions: As the orders of Mucorales do not have well growth in culture media, PCR with tissue specimens is more sensitive than tissue or blood culture methods. Unfortunately, there is no alternative method for direct smear, which is an invasive method. Molecular methods may be helpful in these cases. |
| first_indexed | 2024-12-19T14:11:38Z |
| format | Article |
| id | doaj.art-937fda20ebac47b383b97513e93b1d72 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-12-19T14:11:38Z |
| publishDate | 2013-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-937fda20ebac47b383b97513e93b1d722022-12-21T20:18:06ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472013-12-0154Comparison of histopathological analysis, culture and polymerase chain reaction assays to detect mucormycosis in biopsy and blood specimensParisa Badiee0Amir Arastefar1Hadis Jafarian2Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.Background and Objectives: The aim of this study was to compare direct microscopic examination with culture and PCR for the diagnosis of Mucorales infection in blood and tissue specimens. Material and Methods: Blood samples and tissue specimens were obtained from 28 patients (total 58 samples) with suspected invasive fungal infection and cultured on proper media. Direct smear of tissue samples was done with potassium hydroxide, hematoxylin and eosin, and methenamine silver staining. DNA extracted from blood and tissue specimens were used for semi-nested PCR targeting 18S rDNA of Mucorales species. Results: Mucormycosis was documented in 7/28 (25%) of tissue specimens with positive findings by direct smear, of which PCR and culture were positive in 6 (86%) and 5 (70%) specimens, respectively. The etiologic agents were Mucor spp. and Rhizopus spp. However, culture and PCR results for all blood specimens were negative. Conclusions: As the orders of Mucorales do not have well growth in culture media, PCR with tissue specimens is more sensitive than tissue or blood culture methods. Unfortunately, there is no alternative method for direct smear, which is an invasive method. Molecular methods may be helpful in these cases.https://ijm.tums.ac.ir/index.php/ijm/article/view/48818S rDNAInvasive fungal infectionMucoraceaSemi-nested PCR |
| spellingShingle | Parisa Badiee Amir Arastefar Hadis Jafarian Comparison of histopathological analysis, culture and polymerase chain reaction assays to detect mucormycosis in biopsy and blood specimens Iranian Journal of Microbiology 18S rDNA Invasive fungal infection Mucoracea Semi-nested PCR |
| title | Comparison of histopathological analysis, culture and polymerase chain reaction assays to detect mucormycosis in biopsy and blood specimens |
| title_full | Comparison of histopathological analysis, culture and polymerase chain reaction assays to detect mucormycosis in biopsy and blood specimens |
| title_fullStr | Comparison of histopathological analysis, culture and polymerase chain reaction assays to detect mucormycosis in biopsy and blood specimens |
| title_full_unstemmed | Comparison of histopathological analysis, culture and polymerase chain reaction assays to detect mucormycosis in biopsy and blood specimens |
| title_short | Comparison of histopathological analysis, culture and polymerase chain reaction assays to detect mucormycosis in biopsy and blood specimens |
| title_sort | comparison of histopathological analysis culture and polymerase chain reaction assays to detect mucormycosis in biopsy and blood specimens |
| topic | 18S rDNA Invasive fungal infection Mucoracea Semi-nested PCR |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/488 |
| work_keys_str_mv | AT parisabadiee comparisonofhistopathologicalanalysiscultureandpolymerasechainreactionassaystodetectmucormycosisinbiopsyandbloodspecimens AT amirarastefar comparisonofhistopathologicalanalysiscultureandpolymerasechainreactionassaystodetectmucormycosisinbiopsyandbloodspecimens AT hadisjafarian comparisonofhistopathologicalanalysiscultureandpolymerasechainreactionassaystodetectmucormycosisinbiopsyandbloodspecimens |