Case Report: A Peculiar Case of Inflammatory Colitis After SARS-CoV-2 Infection
We report a case of inflammatory colitis after SARS-CoV-2 infection in a patient with no additional co-morbidity who died within three weeks of hospitalization. As it is becoming increasingly clear that SARS-CoV-2 infection can cause immunological alterations, we investigated the expression of the i...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-02-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.849140/full |
| _version_ | 1828891771464056832 |
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| author | Mariangela Rutigliani Matteo Bozzo Andrea Barberis Marco Greppi Emanuela Anelli Luca Castellaro Alessandro Bonsignore Alessandro Bonsignore Antonio Azzinnaro Silvia Pesce Marco Filauro Gian Andrea Rollandi Patrizio Castagnola Simona Candiani Emanuela Marcenaro |
| author_facet | Mariangela Rutigliani Matteo Bozzo Andrea Barberis Marco Greppi Emanuela Anelli Luca Castellaro Alessandro Bonsignore Alessandro Bonsignore Antonio Azzinnaro Silvia Pesce Marco Filauro Gian Andrea Rollandi Patrizio Castagnola Simona Candiani Emanuela Marcenaro |
| author_sort | Mariangela Rutigliani |
| collection | DOAJ |
| description | We report a case of inflammatory colitis after SARS-CoV-2 infection in a patient with no additional co-morbidity who died within three weeks of hospitalization. As it is becoming increasingly clear that SARS-CoV-2 infection can cause immunological alterations, we investigated the expression of the inhibitory checkpoint PD-1 and its ligand PD-L1 to explore the potential role of this axis in the break of self-tolerance. The presence of the SARS-CoV-2 virus in colon tissue was demonstrated by qRT-PCR and immunohistochemical localization of the nucleocapsid protein. Expression of lymphocyte markers, PD-1, and PD-L1 in colon tissue was investigated by IHC. SARS-CoV-2-immunoreactive cells were detected both in the ulcerated and non-ulcerated mucosal areas. Compared to healthy tissue, where PD-1 is weakly expressed and PD-L1 is absent, PD-1 and PD-L1 expression appears in the inflamed mucosal tissue, as expected, but was mainly confined to non-ulcerative areas. At the same time, these markers were virtually undetectable in areas of mucosal ulceration. Our data show an alteration of the PD-1/PD-L1 axis and suggest a link between SARS-CoV-2 infection and an aberrant autoinflammatory response due to concomitant breakdown of the PD-1/PD-L1 interaction leading to early death of the patient. |
| first_indexed | 2024-12-13T13:23:00Z |
| format | Article |
| id | doaj.art-93814d282302442f9f6428a57110941f |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-13T13:23:00Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-93814d282302442f9f6428a57110941f2022-12-21T23:44:21ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-02-011310.3389/fimmu.2022.849140849140Case Report: A Peculiar Case of Inflammatory Colitis After SARS-CoV-2 InfectionMariangela Rutigliani0Matteo Bozzo1Andrea Barberis2Marco Greppi3Emanuela Anelli4Luca Castellaro5Alessandro Bonsignore6Alessandro Bonsignore7Antonio Azzinnaro8Silvia Pesce9Marco Filauro10Gian Andrea Rollandi11Patrizio Castagnola12Simona Candiani13Emanuela Marcenaro14Department of Laboratory and Service - Histological and Anatomical Pathology Unit, Ente Ospedaliero (E.O.) Galliera Hospital, Genova, ItalyDepartment of Experimental Medicine, University of Genoa, Genova, ItalyDepartment of Abdominal Surgery - General and Hepatopancreatobiliary Surgery Unit, Ente Ospedaliero (E.O.) Galliera Hospital, Genova, ItalyDepartment of Experimental Medicine, University of Genoa, Genova, ItalyDepartment of Laboratory and Service - Histological and Anatomical Pathology Unit, Ente Ospedaliero (E.O.) Galliera Hospital, Genova, ItalyDepartment of Laboratory and Service - Histological and Anatomical Pathology Unit, Ente Ospedaliero (E.O.) Galliera Hospital, Genova, ItalyIRCCS Ospedale Policlinico San Martino, Genova, ItalySection of Legal and Forensic Medicine, Department of Health Sciences, University of Genoa, Genova, ItalyDepartment of Abdominal Surgery - General and Hepatopancreatobiliary Surgery Unit, Ente Ospedaliero (E.O.) Galliera Hospital, Genova, ItalyDepartment of Experimental Medicine, University of Genoa, Genova, ItalyDepartment of Abdominal Surgery - General and Hepatopancreatobiliary Surgery Unit, Ente Ospedaliero (E.O.) Galliera Hospital, Genova, ItalyDepartment of Abdominal Surgery - General and Hepatopancreatobiliary Surgery Unit, Ente Ospedaliero (E.O.) Galliera Hospital, Genova, ItalyIRCCS Ospedale Policlinico San Martino, Genova, ItalyDepartment of Earth, Environment and Life Sciences, University of Genoa, Genova, ItalyDepartment of Experimental Medicine, University of Genoa, Genova, ItalyWe report a case of inflammatory colitis after SARS-CoV-2 infection in a patient with no additional co-morbidity who died within three weeks of hospitalization. As it is becoming increasingly clear that SARS-CoV-2 infection can cause immunological alterations, we investigated the expression of the inhibitory checkpoint PD-1 and its ligand PD-L1 to explore the potential role of this axis in the break of self-tolerance. The presence of the SARS-CoV-2 virus in colon tissue was demonstrated by qRT-PCR and immunohistochemical localization of the nucleocapsid protein. Expression of lymphocyte markers, PD-1, and PD-L1 in colon tissue was investigated by IHC. SARS-CoV-2-immunoreactive cells were detected both in the ulcerated and non-ulcerated mucosal areas. Compared to healthy tissue, where PD-1 is weakly expressed and PD-L1 is absent, PD-1 and PD-L1 expression appears in the inflamed mucosal tissue, as expected, but was mainly confined to non-ulcerative areas. At the same time, these markers were virtually undetectable in areas of mucosal ulceration. Our data show an alteration of the PD-1/PD-L1 axis and suggest a link between SARS-CoV-2 infection and an aberrant autoinflammatory response due to concomitant breakdown of the PD-1/PD-L1 interaction leading to early death of the patient.https://www.frontiersin.org/articles/10.3389/fimmu.2022.849140/fullCOVID-19SARS-CoV-2necrotizing ulcerative colitiscytotoxic immune cellsPD-1/PD-L1 axis |
| spellingShingle | Mariangela Rutigliani Matteo Bozzo Andrea Barberis Marco Greppi Emanuela Anelli Luca Castellaro Alessandro Bonsignore Alessandro Bonsignore Antonio Azzinnaro Silvia Pesce Marco Filauro Gian Andrea Rollandi Patrizio Castagnola Simona Candiani Emanuela Marcenaro Case Report: A Peculiar Case of Inflammatory Colitis After SARS-CoV-2 Infection Frontiers in Immunology COVID-19 SARS-CoV-2 necrotizing ulcerative colitis cytotoxic immune cells PD-1/PD-L1 axis |
| title | Case Report: A Peculiar Case of Inflammatory Colitis After SARS-CoV-2 Infection |
| title_full | Case Report: A Peculiar Case of Inflammatory Colitis After SARS-CoV-2 Infection |
| title_fullStr | Case Report: A Peculiar Case of Inflammatory Colitis After SARS-CoV-2 Infection |
| title_full_unstemmed | Case Report: A Peculiar Case of Inflammatory Colitis After SARS-CoV-2 Infection |
| title_short | Case Report: A Peculiar Case of Inflammatory Colitis After SARS-CoV-2 Infection |
| title_sort | case report a peculiar case of inflammatory colitis after sars cov 2 infection |
| topic | COVID-19 SARS-CoV-2 necrotizing ulcerative colitis cytotoxic immune cells PD-1/PD-L1 axis |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.849140/full |
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