Dietary supplementation of recombinant tilapia piscidin 4-expressing yeast enhances growth and immune response in Lates calcarifer
Marine antimicrobial peptide tilapia piscidin (TP) 4 possesses antibacterial and immune regulatory activities, making it a potentially valuable feed supplement in aquaculture. In this study, we expressed the TP4 peptide, originally identified from Oreochromis niloticus, in yeast (Pichia pastoris) un...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-03-01
|
| Series: | Aquaculture Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513419301693 |
| _version_ | 1819050697316892672 |
|---|---|
| author | Han-Ning Huang Bor-Chyuan Su Tsung-Yu Tsai Venugopal Rajanbabu Chieh-Yu Pan Jyh-Yih Chen |
| author_facet | Han-Ning Huang Bor-Chyuan Su Tsung-Yu Tsai Venugopal Rajanbabu Chieh-Yu Pan Jyh-Yih Chen |
| author_sort | Han-Ning Huang |
| collection | DOAJ |
| description | Marine antimicrobial peptide tilapia piscidin (TP) 4 possesses antibacterial and immune regulatory activities, making it a potentially valuable feed supplement in aquaculture. In this study, we expressed the TP4 peptide, originally identified from Oreochromis niloticus, in yeast (Pichia pastoris) under the control of an alcohol oxidase (Aox) 1 promoter. Recombinant (r)TP4 expression was induced by methanol. Transformation was confirmed by PCR, and expression was confirmed by colony hybridization. For scalable production, transformed yeast was cultured and induced in a bench-top fermenter bioreactor system under controlled conditions. The rTP4-expressing yeast cells were then pelleted and fed to 28-day-old Lates calcarifer as a 0, 1, 3 or 5 % supplement to the normal diet. Growth performance and immune response were monitored in the fish for four weeks. The 3 and 5 % rTP4-yeast groups showed significant improvements in weight gain, feed efficiency, survival, protein efficiency ratio, and hepatosomatic index. In addition, rTP4-yeast increased the levels of IgM, which plays a critical role in innate immunity. Furthermore, rTP4-yeast-containing diet elevated SOD, GPx and catalase activities. Since feeding L. calcarifer with bioreactor-produced rTP4-expressing yeast significantly enhanced growth and immunity, rTP4 should be considered for use as a functional feed supplement to replace excessively administered antibiotics in farmed fish. Keyword: Tilapia |
| first_indexed | 2024-12-21T11:52:10Z |
| format | Article |
| id | doaj.art-9385b7748dbb4683b58318bf6832354b |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-12-21T11:52:10Z |
| publishDate | 2020-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-9385b7748dbb4683b58318bf6832354b2022-12-21T19:05:01ZengElsevierAquaculture Reports2352-51342020-03-0116Dietary supplementation of recombinant tilapia piscidin 4-expressing yeast enhances growth and immune response in Lates calcariferHan-Ning Huang0Bor-Chyuan Su1Tsung-Yu Tsai2Venugopal Rajanbabu3Chieh-Yu Pan4Jyh-Yih Chen5Genomics Biotechnology Co., Ltd, 14F., No.100, Sec. 1, Xintai 5th Rd., Xizhi Dist., New Taipei City TaiwanDepartment of Anatomy and Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, TaiwanMarine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, 23-10 Dahuen Road, Jiaushi, Ilan 262, TaiwanAnbil Dharmalingam Agricultural College and Research Institute, Tamil Nadu Agricultural University, Tiruchchirapalli 620027, Tamil Nadu, IndiaDepartment and Graduate Institute of Aquaculture, National Kaohsiung University of Science and Technology, Kaohsiung 811, TaiwanMarine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, 23-10 Dahuen Road, Jiaushi, Ilan 262, Taiwan; Corresponding author.Marine antimicrobial peptide tilapia piscidin (TP) 4 possesses antibacterial and immune regulatory activities, making it a potentially valuable feed supplement in aquaculture. In this study, we expressed the TP4 peptide, originally identified from Oreochromis niloticus, in yeast (Pichia pastoris) under the control of an alcohol oxidase (Aox) 1 promoter. Recombinant (r)TP4 expression was induced by methanol. Transformation was confirmed by PCR, and expression was confirmed by colony hybridization. For scalable production, transformed yeast was cultured and induced in a bench-top fermenter bioreactor system under controlled conditions. The rTP4-expressing yeast cells were then pelleted and fed to 28-day-old Lates calcarifer as a 0, 1, 3 or 5 % supplement to the normal diet. Growth performance and immune response were monitored in the fish for four weeks. The 3 and 5 % rTP4-yeast groups showed significant improvements in weight gain, feed efficiency, survival, protein efficiency ratio, and hepatosomatic index. In addition, rTP4-yeast increased the levels of IgM, which plays a critical role in innate immunity. Furthermore, rTP4-yeast-containing diet elevated SOD, GPx and catalase activities. Since feeding L. calcarifer with bioreactor-produced rTP4-expressing yeast significantly enhanced growth and immunity, rTP4 should be considered for use as a functional feed supplement to replace excessively administered antibiotics in farmed fish. Keyword: Tilapiahttp://www.sciencedirect.com/science/article/pii/S2352513419301693 |
| spellingShingle | Han-Ning Huang Bor-Chyuan Su Tsung-Yu Tsai Venugopal Rajanbabu Chieh-Yu Pan Jyh-Yih Chen Dietary supplementation of recombinant tilapia piscidin 4-expressing yeast enhances growth and immune response in Lates calcarifer Aquaculture Reports |
| title | Dietary supplementation of recombinant tilapia piscidin 4-expressing yeast enhances growth and immune response in Lates calcarifer |
| title_full | Dietary supplementation of recombinant tilapia piscidin 4-expressing yeast enhances growth and immune response in Lates calcarifer |
| title_fullStr | Dietary supplementation of recombinant tilapia piscidin 4-expressing yeast enhances growth and immune response in Lates calcarifer |
| title_full_unstemmed | Dietary supplementation of recombinant tilapia piscidin 4-expressing yeast enhances growth and immune response in Lates calcarifer |
| title_short | Dietary supplementation of recombinant tilapia piscidin 4-expressing yeast enhances growth and immune response in Lates calcarifer |
| title_sort | dietary supplementation of recombinant tilapia piscidin 4 expressing yeast enhances growth and immune response in lates calcarifer |
| url | http://www.sciencedirect.com/science/article/pii/S2352513419301693 |
| work_keys_str_mv | AT hanninghuang dietarysupplementationofrecombinanttilapiapiscidin4expressingyeastenhancesgrowthandimmuneresponseinlatescalcarifer AT borchyuansu dietarysupplementationofrecombinanttilapiapiscidin4expressingyeastenhancesgrowthandimmuneresponseinlatescalcarifer AT tsungyutsai dietarysupplementationofrecombinanttilapiapiscidin4expressingyeastenhancesgrowthandimmuneresponseinlatescalcarifer AT venugopalrajanbabu dietarysupplementationofrecombinanttilapiapiscidin4expressingyeastenhancesgrowthandimmuneresponseinlatescalcarifer AT chiehyupan dietarysupplementationofrecombinanttilapiapiscidin4expressingyeastenhancesgrowthandimmuneresponseinlatescalcarifer AT jyhyihchen dietarysupplementationofrecombinanttilapiapiscidin4expressingyeastenhancesgrowthandimmuneresponseinlatescalcarifer |