Hemolymph Ecdysteroid Titer Affects Maternal mRNAs during <i>Bombyx mori</i> Oogenesis
Silkworm larval–pupal metamorphosis and the first half of pupal–adult development occur during oogenesis from previtellogenesis to vitellogenesis and include two peaks of the hemolymph ecdysteroid titer. Moreover, a rise in 20-hydroxyecdysone titer in early pupae can trigger the first major transiti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/11/969 |
| _version_ | 1827676438034120704 |
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| author | Meirong Zhang Pingzhen Xu Tao Chen |
| author_facet | Meirong Zhang Pingzhen Xu Tao Chen |
| author_sort | Meirong Zhang |
| collection | DOAJ |
| description | Silkworm larval–pupal metamorphosis and the first half of pupal–adult development occur during oogenesis from previtellogenesis to vitellogenesis and include two peaks of the hemolymph ecdysteroid titer. Moreover, a rise in 20-hydroxyecdysone titer in early pupae can trigger the first major transition from previtellogenesis to vitellogenesis in silkworm oogenesis. In this study, we first investigated the expression patterns of 66 maternal genes in the ovary at the wandering stage. We then examined the developmental expression profiles in six time-series samples of ovaries or ovarioles by reverse transcription–quantitative PCR. We found that the transcripts of 22 maternal genes were regulated by 20-hydroxyecdysone in the isolated abdomens of the pupae following a single injection of 20-hydroxyecdysone. This study is the first to determine the relationship between 20-hydroxyecdysone and maternal genes during silkworm oogenesis. These findings provide a basis for further research into the embryonic development of <i>Bombyx mori</i>. |
| first_indexed | 2024-03-10T05:24:53Z |
| format | Article |
| id | doaj.art-93874b1457934a648957a530caeaaa72 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T05:24:53Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-93874b1457934a648957a530caeaaa722023-11-22T23:46:31ZengMDPI AGInsects2075-44502021-10-01121196910.3390/insects12110969Hemolymph Ecdysteroid Titer Affects Maternal mRNAs during <i>Bombyx mori</i> OogenesisMeirong Zhang0Pingzhen Xu1Tao Chen2Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212028, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212028, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212028, ChinaSilkworm larval–pupal metamorphosis and the first half of pupal–adult development occur during oogenesis from previtellogenesis to vitellogenesis and include two peaks of the hemolymph ecdysteroid titer. Moreover, a rise in 20-hydroxyecdysone titer in early pupae can trigger the first major transition from previtellogenesis to vitellogenesis in silkworm oogenesis. In this study, we first investigated the expression patterns of 66 maternal genes in the ovary at the wandering stage. We then examined the developmental expression profiles in six time-series samples of ovaries or ovarioles by reverse transcription–quantitative PCR. We found that the transcripts of 22 maternal genes were regulated by 20-hydroxyecdysone in the isolated abdomens of the pupae following a single injection of 20-hydroxyecdysone. This study is the first to determine the relationship between 20-hydroxyecdysone and maternal genes during silkworm oogenesis. These findings provide a basis for further research into the embryonic development of <i>Bombyx mori</i>.https://www.mdpi.com/2075-4450/12/11/969<i>Bombyx mori</i>maternal mRNAs20-hydroxyecdysonepupawandering |
| spellingShingle | Meirong Zhang Pingzhen Xu Tao Chen Hemolymph Ecdysteroid Titer Affects Maternal mRNAs during <i>Bombyx mori</i> Oogenesis Insects <i>Bombyx mori</i> maternal mRNAs 20-hydroxyecdysone pupa wandering |
| title | Hemolymph Ecdysteroid Titer Affects Maternal mRNAs during <i>Bombyx mori</i> Oogenesis |
| title_full | Hemolymph Ecdysteroid Titer Affects Maternal mRNAs during <i>Bombyx mori</i> Oogenesis |
| title_fullStr | Hemolymph Ecdysteroid Titer Affects Maternal mRNAs during <i>Bombyx mori</i> Oogenesis |
| title_full_unstemmed | Hemolymph Ecdysteroid Titer Affects Maternal mRNAs during <i>Bombyx mori</i> Oogenesis |
| title_short | Hemolymph Ecdysteroid Titer Affects Maternal mRNAs during <i>Bombyx mori</i> Oogenesis |
| title_sort | hemolymph ecdysteroid titer affects maternal mrnas during i bombyx mori i oogenesis |
| topic | <i>Bombyx mori</i> maternal mRNAs 20-hydroxyecdysone pupa wandering |
| url | https://www.mdpi.com/2075-4450/12/11/969 |
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