Bacteriophage vB_SepP_134 and Endolysin LysSte_134_1 as Potential Staphylococcus-Biofilm-Removing Biological Agents
Bacteria of the genus <i>Staphylococcus</i> are significant challenge for medicine, as many species are resistant to multiple antibiotics and some are even to all of the antibiotics we use. One of the approaches to developing new therapeutics to treat staphylococcal infections is the use...
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| Format: | Article |
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MDPI AG
2024-02-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/16/3/385 |
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| author | Natalia N. Golosova Andrey L. Matveev Nina V. Tikunova Yana A. Khlusevich Yulia N. Kozlova Vera V. Morozova Igor V. Babkin Tatiana A. Ushakova Elena V. Zhirakovskaya Elizaveta A. Panina Elena I. Ryabchikova Artem Y. Tikunov |
| author_facet | Natalia N. Golosova Andrey L. Matveev Nina V. Tikunova Yana A. Khlusevich Yulia N. Kozlova Vera V. Morozova Igor V. Babkin Tatiana A. Ushakova Elena V. Zhirakovskaya Elizaveta A. Panina Elena I. Ryabchikova Artem Y. Tikunov |
| author_sort | Natalia N. Golosova |
| collection | DOAJ |
| description | Bacteria of the genus <i>Staphylococcus</i> are significant challenge for medicine, as many species are resistant to multiple antibiotics and some are even to all of the antibiotics we use. One of the approaches to developing new therapeutics to treat staphylococcal infections is the use of bacteriophages specific to these bacteria or the lytic enzymes of such bacteriophages, which are capable of hydrolyzing the cell walls of these bacteria. In this study, a new bacteriophage vB_SepP_134 (St 134) specific to <i>Staphylococcus epidermidis</i> was described. This podophage, with a genome of 18,275 bp, belongs to the <i>Andhravirus</i> genus. St 134 was able to infect various strains of 12 of the 21 tested coagulase-negative <i>Staphylococcus</i> species and one clinical strain from the <i>Staphylococcus aureus</i> complex. The genes encoding endolysin (LysSte134_1) and tail tip lysin (LysSte134_2) were identified in the St 134 genome. Both enzymes were cloned and produced in <i>Escherichia coli</i> cells. The endolysin LysSte134_1 demonstrated catalytic activity against peptidoglycans isolated from <i>S. aureus, S. epidermidis</i>, <i>Staphylococcus haemolyticus</i>, and <i>Staphylococcus warneri</i>. LysSte134_1 was active against <i>S. aureus</i> and <i>S. epidermidis</i> planktonic cells and destroyed the biofilms formed by clinical strains of <i>S. aureus</i> and <i>S. epidermidis</i>. |
| first_indexed | 2024-04-24T17:46:19Z |
| format | Article |
| id | doaj.art-938888f3f07c442793effbf137af1d76 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-04-24T17:46:19Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-938888f3f07c442793effbf137af1d762024-03-27T14:07:44ZengMDPI AGViruses1999-49152024-02-0116338510.3390/v16030385Bacteriophage vB_SepP_134 and Endolysin LysSte_134_1 as Potential Staphylococcus-Biofilm-Removing Biological AgentsNatalia N. Golosova0Andrey L. Matveev1Nina V. Tikunova2Yana A. Khlusevich3Yulia N. Kozlova4Vera V. Morozova5Igor V. Babkin6Tatiana A. Ushakova7Elena V. Zhirakovskaya8Elizaveta A. Panina9Elena I. Ryabchikova10Artem Y. Tikunov11Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaBacteria of the genus <i>Staphylococcus</i> are significant challenge for medicine, as many species are resistant to multiple antibiotics and some are even to all of the antibiotics we use. One of the approaches to developing new therapeutics to treat staphylococcal infections is the use of bacteriophages specific to these bacteria or the lytic enzymes of such bacteriophages, which are capable of hydrolyzing the cell walls of these bacteria. In this study, a new bacteriophage vB_SepP_134 (St 134) specific to <i>Staphylococcus epidermidis</i> was described. This podophage, with a genome of 18,275 bp, belongs to the <i>Andhravirus</i> genus. St 134 was able to infect various strains of 12 of the 21 tested coagulase-negative <i>Staphylococcus</i> species and one clinical strain from the <i>Staphylococcus aureus</i> complex. The genes encoding endolysin (LysSte134_1) and tail tip lysin (LysSte134_2) were identified in the St 134 genome. Both enzymes were cloned and produced in <i>Escherichia coli</i> cells. The endolysin LysSte134_1 demonstrated catalytic activity against peptidoglycans isolated from <i>S. aureus, S. epidermidis</i>, <i>Staphylococcus haemolyticus</i>, and <i>Staphylococcus warneri</i>. LysSte134_1 was active against <i>S. aureus</i> and <i>S. epidermidis</i> planktonic cells and destroyed the biofilms formed by clinical strains of <i>S. aureus</i> and <i>S. epidermidis</i>.https://www.mdpi.com/1999-4915/16/3/385bacteriophageendolysin<i>Staphylococcus epidermidis</i><i>Staphylococcus aureus</i>antimicrobial agentbiofilm |
| spellingShingle | Natalia N. Golosova Andrey L. Matveev Nina V. Tikunova Yana A. Khlusevich Yulia N. Kozlova Vera V. Morozova Igor V. Babkin Tatiana A. Ushakova Elena V. Zhirakovskaya Elizaveta A. Panina Elena I. Ryabchikova Artem Y. Tikunov Bacteriophage vB_SepP_134 and Endolysin LysSte_134_1 as Potential Staphylococcus-Biofilm-Removing Biological Agents Viruses bacteriophage endolysin <i>Staphylococcus epidermidis</i> <i>Staphylococcus aureus</i> antimicrobial agent biofilm |
| title | Bacteriophage vB_SepP_134 and Endolysin LysSte_134_1 as Potential Staphylococcus-Biofilm-Removing Biological Agents |
| title_full | Bacteriophage vB_SepP_134 and Endolysin LysSte_134_1 as Potential Staphylococcus-Biofilm-Removing Biological Agents |
| title_fullStr | Bacteriophage vB_SepP_134 and Endolysin LysSte_134_1 as Potential Staphylococcus-Biofilm-Removing Biological Agents |
| title_full_unstemmed | Bacteriophage vB_SepP_134 and Endolysin LysSte_134_1 as Potential Staphylococcus-Biofilm-Removing Biological Agents |
| title_short | Bacteriophage vB_SepP_134 and Endolysin LysSte_134_1 as Potential Staphylococcus-Biofilm-Removing Biological Agents |
| title_sort | bacteriophage vb sepp 134 and endolysin lysste 134 1 as potential staphylococcus biofilm removing biological agents |
| topic | bacteriophage endolysin <i>Staphylococcus epidermidis</i> <i>Staphylococcus aureus</i> antimicrobial agent biofilm |
| url | https://www.mdpi.com/1999-4915/16/3/385 |
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